CTR:Notebook/Migrant/2016/09/26: Difference between revisions
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**Take 8 uL from each well and combine to 1.7 mL tube, split entire volume into 2 separate tubes | **Take 8 uL from each well and combine to 1.7 mL tube, split entire volume into 2 separate tubes | ||
**AMPure bead clean up: | **AMPure bead clean up: | ||
***Used | ***Used 365 uL beads to DNA (1:1), per tube | ||
***2 washes of 800 uL 80% EtOH each | ***2 washes of 800 uL 80% EtOH each | ||
***Elute in 105 uL Low TE per tube | ***Elute in 105 uL Low TE per tube | ||
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**BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | ||
**Combined both tubes of sheared DNA and ran on a gel | **Combined both tubes of sheared DNA and ran on a gel | ||
[[Image: | [[Image:Zola Sheared 09292016.JPG|60px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
3) 2uL Silvereye sheared DNA | 3) 2uL Silvereye sheared DNA |
Revision as of 12:24, 1 October 2016
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BestRAD Library Prep - Silvereye Plate 2
1) 2uL 100bp low scale ladder, 3) 2uL Silvereye sheared DNA
1) 2uL 100bp low scale ladder 2) 5uL Silvereye PCR product
1) 2uL 100bp low scale ladder 2) 5uL Silvereye PCR product
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