CHE.496/2008/Projects/2/Group 2: Difference between revisions

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**Pathway: 2 acetyl-CoA are condensed into acetoacetyl-CoA by phaA.  Then, phaB (NADPH-dependent) reduces this to (R)-3-hydroxybutyryl-CoA.  Finally, this is polymerized into PHB by phaC.
**Pathway: 2 acetyl-CoA are condensed into acetoacetyl-CoA by phaA.  Then, phaB (NADPH-dependent) reduces this to (R)-3-hydroxybutyryl-CoA.  Finally, this is polymerized into PHB by phaC.
**''phaA'' (code for acetyl-CoA acetyltransferase) [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_008313.1&from=1559207&to=1560388&dopt=fasta Nucleotide sequence]
**''phaA'' (code for acetyl-CoA acetyltransferase) [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_008313.1&from=1559207&to=1560388&dopt=fasta Nucleotide sequence]
**Possible use in tissue engineering applications
**Possible use in tissue engineering applications (PHB is found in blood plasma)
**Chassis: ''E. coli''
**Chassis: ''E. coli''
*Biological degradation of plastic
*Biological degradation of plastic
**PHAs are biodegradable.
**PHAs are biodegradable.

Revision as of 11:29, 28 March 2008

CHE.496: Biological Systems Design Seminar

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Group 2's project overview and part design

List of initial ideas

  • Foundational
    • Parts for genetic circuits
    • New chassis (environment, etc.)
    • Optimizing existing parts (e.g., inverter)
    • Light sensor
    • Compartmentalization/insulation
    • Molecular screens (filters) that enable signal isolation and insulation (inside the cells and in between cells)
  • Computational
    • modeling of biotech-relevant microorganisms
  • Synthetic Biology Tools
    • Quality characterization techniques
    • Ways to insulate biological signals
  • Metabolic Engineering
    • Polysaccharide synthesis (for food)
    • Bioplastic production
    • Chemical degradation (toxin breakdown)
      • Oil
      • CO2
      • Plastics
  • Genetic Circuit Engineering
    • Light-based repressilator
    • Light switch
  • Biosensing
    • Ethylene biosensor
    • Algae that sense light and displays permanent memory

List of top two project ideas

  • Biological production of plastic
    • Polyhydroxyalkanoates (PHAs) are naturally synthesized polyesters in Ralstonia eutropha
    • These PHAs are used for energy storage
    • Polyhydroxybutyrate (PHB) is a PHA and could possible replace polypropylene
    • Biologically-produced plastics represent a sustainable chemical industry that is not dependent on petroleum
    • Three genes necessary for PHB synthesis: phaA, phaB and phaC (from Pohlmann et al., 2006)
    • Pathway: 2 acetyl-CoA are condensed into acetoacetyl-CoA by phaA. Then, phaB (NADPH-dependent) reduces this to (R)-3-hydroxybutyryl-CoA. Finally, this is polymerized into PHB by phaC.
    • phaA (code for acetyl-CoA acetyltransferase) Nucleotide sequence
    • Possible use in tissue engineering applications (PHB is found in blood plasma)
    • Chassis: E. coli
  • Biological degradation of plastic
    • PHAs are biodegradable.
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