CH391L/S13/Probiotics

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Probiotics

Introduction

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Directed evolution of a thermostable esterase

iGEM link

References

1. Romero PA and Arnold FH. Exploring protein fitness landscapes by directed evolution. Nat Rev Mol Cell Bio, 2009. [Romero2009]
2. Crameri A, Whitehorn EA, Tate E, Stemmer WP. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol, 1996.

   [Stemmer1995]

3. Giver L, Gershenson A, Freskgard PO, Arnold FH. Directed evolution of a thermostable esterase. Proc Natl Acad Sci USA, 1998.

   [Giver1998]

4. Cadwell RC and Joyce GF. Randomization of genes by PCR mutagenesis. Genome Res, 1992.

   [Cadwell1992]

5. Abou-Nader M and Benedik MJ. Rapid generation of random mutant libraries. Bioeng Bugs, 2010.

   [Abou-Nader2010]

6. Stemmer WP. Rapid evolution of a protein by in vitro DNA shuffling. Nature, 1994.

   [Stemmer1994]

7. Lin H and Cornish VW. Screening and selection methods for large-scale analysis of protein function.

   [Lin2002]

8. Leemhuis H, Kelly RM, Dijkhuizen L. Directed evolution of enzymes: library screening strategies. IUBMB Life, 2009.

   [Leemhuis2009]