CH391L/S13/In vitro Selection of FNAs

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==Introduction==
==Introduction==
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Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues)  that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.
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==Types of Functional Nucleic Acids==
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==Functional Nucleic Acids==
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==Aptamers==
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Revision as of 20:17, 10 February 2013


Contents

Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


Functional Nucleic Acids

In vitro Selection of Functional Nucleic Acids

Ribozymes

Deoxyribozymes

Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

Image:CH391L_S12_Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

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