CH391L/S13/In vitro Selection of FNAs

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(New page: Category:CH391L_S13 ==Introduction== ==Types of Functional Nucleic Acids== <!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some co...)
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[[Category:CH391L_S13]]
[[Category:CH391L_S13]]
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==Introduction==
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==Introduction==
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==Types of Functional Nucleic Acids==
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==In vitro Selection of Functional Nucleic Acids==
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==Ribozymes==
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==Types of Functional Nucleic Acids==
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==Deoxyribozymes==
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 +
==Aptamers==
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==Extra==
<!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some combination of short DNA oligonucleotides. These oligonucleotides are later assembled into a complementary DNA duplex, amplified and inserted into their final genetic context.
<!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some combination of short DNA oligonucleotides. These oligonucleotides are later assembled into a complementary DNA duplex, amplified and inserted into their final genetic context.
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Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
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==In vitro Selection of Functional Nucleic Acids==
 
-
 
-
==Ribozymes==
 
-
 
-
==Deoxyribozymes==
 
-
 
-
==Aptamers==
 

Revision as of 17:43, 8 February 2013


Contents

Introduction

Types of Functional Nucleic Acids

In vitro Selection of Functional Nucleic Acids

Ribozymes

Deoxyribozymes

Aptamers

Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

Image:CH391L_S12_Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

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