The ability to introduce exogenous DNA into an organism to alter its genetic program is one of the most crucial tools in modern biology. Early work showed that certain bacteria could acquire the traits of a related strain through the addition of heat-killed cells. Although it was not well understood at the time, the transfer of gene-encoding DNA from one strain to another facilitated this. This concept was turned into a useful tool upon the advent of bacterial plasmid transformations in the early 1970's, which allowed genes of interest to be easily inserted into E. coli. Over the years, methods have been developed to introduce exogenous genes into a wide range of useful organisms, including bacteria, yeasts, plants, and animal tissues. These methods vary enormously in efficiency however, necessitating a way to identify and isolate cells which contain the DNA of interest. This can be accomplished either by screening for successfully modified cells, or through selection.
Screening vs. Selection
In the case of bacterial transformations, it's possible to plate cells post-transformation on non-selective media and screen each individual colony to identify those that have been successfully modified. Because each colony arises from a single cell present during transformation, the colony is made up of a set of identical clones. Screening can involve testing for the desired DNA itself or for the product of an inserted gene. This process is extremely difficult however, as the vast majority of cells will not be successfully transformed, so many colonies need to be tested to identify even a single transformant. A much more efficient strategy is to use a selectable genetic marker that allows only those cells which have been transformed to survive under certain growth conditions. These marker genes may be combined with the DNA of interest on a single plasmid, thus ensuring that any cells that survive selection contain the gene(s) of interest. The most commonly used selectable markers in bacteria are genes that provide resistance to a specific antibiotic upon transformation, allowing for positive selection of cells containing the marker.
Antibiotic Resistance Markers
Novel Marker Strategies
TetA Dual Genetic Selection
- Muranaka N, Sharma V, Nomura Y, and Yokobayashi Y. . pmid:19190095.
Riboswitch selection/screening using a tetA-GFP fusion marker