CH391L/S13/In vitro Selection of FNAs

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<!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some combination of short DNA oligonucleotides. These oligonucleotides are later assembled into a complementary DNA duplex, amplified and inserted into their final genetic context.
<!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some combination of short DNA oligonucleotides. These oligonucleotides are later assembled into a complementary DNA duplex, amplified and inserted into their final genetic context.
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http://med.stanford.edu/sgtc/resources/images/Chemical_DNA_Synthesis_Chart_3-9-10_revised_PW.pdf -->
 
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Oligonucleotides are [http://en.wikipedia.org/wiki/Oligonucleotide_synthesis chemically synthesized] from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.
 
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[[Image:CH391L_S12_Phosphoramidite.png]]
 
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Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
 
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Revision as of 05:27, 11 February 2013


Contents

Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


Functional Nucleic Acids

[1],[2]

Ribozymes

Deoxyribozymes

Image:8-17OP.png


Aptamers and Riboswitches

In vitro Selection of Functional Nucleic Acids





Extra

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