CH391L/S13/DnaAssembly

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Revision as of 20:18, 21 January 2013

Introduction

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

IDT Gene Blocks
DNA2.0
invitrogen (life technologies, geneart)
genscript
celtek
biobasic
sigma?
GENEWIZ
biomatik
OriGene (blue heron)
Epoch Life Science

Cloning Techniques

TOPO TA cloning (invitrogen)

Restriction Enzyme

-BioBricks
-BglBricks

in vivo techniques

• e coli
  
• s. cerevisiae
  

Recombination

-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]

References

1. Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. . pmid:21364738. PubMed HubMed [Weber2011]

   MoClo

2. Hughes RA, Miklos AE, and Ellington AD. . pmid:21601682. PubMed HubMed [Hughes2011]

   Gene Synthesis Review

3. Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. . pmid:22126803. PubMed HubMed [Werner2012]

   MoClo

4. Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. . pmid:21750718. PubMed HubMed [SarrionPerdigones2011]

   GoldenBraid

5. Engler C, Kandzia R, and Marillonnet S. . pmid:18985154. PubMed HubMed [Engler2008]

   GoldenGate

6. Quan J and Tian J. . pmid:19649325. PubMed HubMed [Quan2009]

   CPEC

7. Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:18218864. PubMed HubMed [Gibson2008]
8. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:19363495. PubMed HubMed [Gibson2009]

   T5 exonuclease recombination

9. Li MZ and Elledge SJ. . pmid:17293868. PubMed HubMed [Li2007]

   SLIC

10. Li MZ and Elledge SJ. . pmid:22328425. PubMed HubMed [Li2012]

    SLIC

11. Sleight SC, Bartley BA, Lieviant JA, and Sauro HM. . pmid:20385581. PubMed HubMed [Sleight2010]

    In-Fusion biobrick

12. Zhu B, Cai G, Hall EO, and Freeman GJ. . pmid:17907578. PubMed HubMed [Zhu2007]

    in-fusion

13. Benoit RM, Wilhelm RN, Scherer-Becker D, and Ostermeier C. . pmid:16289702. PubMed HubMed [Benoit2006]

    in-fusion

14. Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646. PubMed HubMed [GeuFlores2007]

    USER

15. Gibson DG. . pmid:19745056. PubMed HubMed [Gibson2009-2]

    oligonucleotide assembly

16. Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375. PubMed HubMed [Horton2009]

    SOEing

17. Czar MJ, Anderson JC, Bader JS, and Peccoud J. . pmid:19111926. PubMed HubMed [Czar2009]

    review

18. Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL. . pmid:7590320. PubMed HubMed [Stemmer1995]

    PCA

19. Aslanidis C and de Jong PJ. . pmid:2235490. PubMed HubMed [Aslanidis1990]

    LIC

20. Aslanidis C, de Jong PJ, and Schmitz G. . pmid:7580902. PubMed HubMed [Aslanidis1994]

    LIC

21. Li C and Evans RM. . pmid:9321675. PubMed HubMed [Li1997]

    LIC

22. Angrand PO, Daigle N, van der Hoeven F, Schöler HR, and Stewart AF. . pmid:10446259. PubMed HubMed [Angrand1999]

    lambda Red recombinase

23. Hartley JL, Temple GF, and Brasch MA. . pmid:11076863. PubMed HubMed [Hartley2000]

    Gateway lambda Int

24. Khalil AM, Julius JA, and Bachant J. . pmid:17702758. PubMed HubMed [Khalil2007]

    Gateway lambda Cre

25. Larionov V, Kouprina N, Graves J, Chen XN, Korenberg JR, and Resnick MA. . pmid:8552668. PubMed HubMed [Larionov1996]

    Transformation-associated recombination (TAR) cloning