CH391L/S13/DnaAssembly

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(Gene Synthesis)
(Gene Synthesis)
Line 11: Line 11:
IDT Gene Blocks <br>
IDT Gene Blocks <br>
DNA2.0 <br>
DNA2.0 <br>
-
invitrogen <br>
+
invitrogen (life technologies, geneart) <br>
genscript <br>
genscript <br>
celtek <br>
celtek <br>
biobasic <br>
biobasic <br>
sigma? <br>
sigma? <br>
 +
GENEWIZ <br>
 +
biomatik <br>
 +
OriGene (blue heron) <br>
==Cloning Techniques==
==Cloning Techniques==

Revision as of 19:38, 21 January 2013


Contents

Introduction

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

IDT Gene Blocks
DNA2.0
invitrogen (life technologies, geneart)
genscript
celtek
biobasic
sigma?
GENEWIZ
biomatik
OriGene (blue heron)

Cloning Techniques

TOPO TA cloning (invitrogen)

Restriction Enzyme

-BioBricks
-BglBricks


in vivo techniques

  • e coli
  • s. cerevisiae

Recombination

-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]

References

  1. Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. . pmid:21364738. PubMed HubMed [Weber2011]
    MoClo

  2. Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. . pmid:22126803. PubMed HubMed [Werner2012]
    MoClo

  3. Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. . pmid:21750718. PubMed HubMed [SarrionPerdigones2011]
    GoldenBraid

  4. Engler C, Kandzia R, and Marillonnet S. . pmid:18985154. PubMed HubMed [Engler2008]
    GoldenGate

  5. Quan J and Tian J. . pmid:19649325. PubMed HubMed [Quan2009]
    CPEC

  6. Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:18218864. PubMed HubMed [Gibson2008]
  7. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:19363495. PubMed HubMed [Gibson2009]
    T5 exonuclease recombination

  8. Li MZ and Elledge SJ. . pmid:17293868. PubMed HubMed [Li2007]
    SLIC

  9. Li MZ and Elledge SJ. . pmid:22328425. PubMed HubMed [Li2012]
    SLIC

  10. Sleight SC, Bartley BA, Lieviant JA, and Sauro HM. . pmid:20385581. PubMed HubMed [Sleight2010]
    In-Fusion biobrick

  11. Zhu B, Cai G, Hall EO, and Freeman GJ. . pmid:17907578. PubMed HubMed [Zhu2007]
    in-fusion

  12. Benoit RM, Wilhelm RN, Scherer-Becker D, and Ostermeier C. . pmid:16289702. PubMed HubMed [Benoit2006]
    in-fusion

  13. Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646. PubMed HubMed [GeuFlores2007]
    USER

  14. Gibson DG. . pmid:19745056. PubMed HubMed [Gibson2009-2]
    oligonucleotide assembly

  15. Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375. PubMed HubMed [Horton2009]
    SOEing

  16. Czar MJ, Anderson JC, Bader JS, and Peccoud J. . pmid:19111926. PubMed HubMed [Czar2009]
    review

  17. Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL. . pmid:7590320. PubMed HubMed [Stemmer1995]
    PCA

  18. Aslanidis C and de Jong PJ. . pmid:2235490. PubMed HubMed [Aslanidis1990]
    LIC

  19. Aslanidis C, de Jong PJ, and Schmitz G. . pmid:7580902. PubMed HubMed [Aslanidis1994]
    LIC

  20. Li C and Evans RM. . pmid:9321675. PubMed HubMed [Li1997]
    LIC

  21. Angrand PO, Daigle N, van der Hoeven F, Schöler HR, and Stewart AF. . pmid:10446259. PubMed HubMed [Angrand1999]
    lambda Red recombinase

  22. Hartley JL, Temple GF, and Brasch MA. . pmid:11076863. PubMed HubMed [Hartley2000]
    Gateway lambda Int

  23. Khalil AM, Julius JA, and Bachant J. . pmid:17702758. PubMed HubMed [Khalil2007]
    Gateway lambda Cre

  24. Larionov V, Kouprina N, Graves J, Chen XN, Korenberg JR, and Resnick MA. . pmid:8552668. PubMed HubMed [Larionov1996]
    Transformation-associated recombination (TAR) cloning

All Medline abstracts: PubMed HubMed
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