CH391L/S13/DnaAssembly
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Revision as of 18:13, 21 January 2013
Contents |
Introduction
Oligonucleotide Synthesis
Gene Synthesis
IDT Gene Blocks
Cloning Techniques
TOPO TA cloning (invitrogen)
Restriction Enzyme
-BioBricks
-BglBricks
in vivo techniques
- e coli
- s. cerevisiae
Recombination
-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]
References
- Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. . pmid:21364738.
MoClo - Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. . pmid:22126803.
MoClo - Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. . pmid:21750718.
GoldenBraid - Engler C, Kandzia R, and Marillonnet S. . pmid:18985154.
GoldenGate - Quan J and Tian J. . pmid:19649325.
CPEC - Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:19363495.
T5 exonuclease recombination - Li MZ and Elledge SJ. . pmid:17293868.
SLIC - Li MZ and Elledge SJ. . pmid:22328425.
SLIC - Sleight SC, Bartley BA, Lieviant JA, and Sauro HM. . pmid:20385581.
In-Fusion biobrick - Zhu B, Cai G, Hall EO, and Freeman GJ. . pmid:17907578.
in-fusion - Benoit RM, Wilhelm RN, Scherer-Becker D, and Ostermeier C. . pmid:16289702.
in-fusion - Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646.
USER - Gibson DG. . pmid:19745056.
oligonucleotide assembly - Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375.
SOEing - Czar MJ, Anderson JC, Bader JS, and Peccoud J. . pmid:19111926.
review - Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL. . pmid:7590320.
PCA
