CH391L/S12/In vitro Selection

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Overview of in vitro selection

Library Generation

Randomized Oligodeoxynucleotides
Mutagenic PCR
Gene Shuffling
Neutral Drift

Confinement of Function

Binding
Cis-action
Cellularization
In vitro Compartmentalization

Increased Representation

Binding
Protection
Selective Amplification
FACS

History

Nucleic Acids

Aptamers

Ellington 1990

Ribozymes

Bartel 1993

Proteins

Antibodies

Accepting suggestions

DNA Modifying Proteins

Tawfik 1998

Other Enzymatic Functions

Griffiths 2003 Griffiths 2003 Schema

Self-Replication

Ghadessy 2001


References

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