CAPS - Revision history http://openwetware.org/index.php?title=CAPS&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Wed, 22 May 2013 17:57:19 GMT Nessie Van Loan: had conflicting pH's, fixed http://openwetware.org/index.php?title=CAPS&diff=306954&oldid=prev <p>had conflicting pH's, fixed</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:04, 6 May 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 11.066g CAPS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 11.066g CAPS</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 4.5L 10% Methanol</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 4.5L 10% Methanol</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Adjust pH to <del class="diffchange diffchange-inline">7.4 </del>with around 5mL <del class="diffchange diffchange-inline">concentrated HCl</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Adjust pH to <ins class="diffchange diffchange-inline">11 </ins>with around 5mL <ins class="diffchange diffchange-inline">10N NaOH</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 10% Methanol to 5L</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 10% Methanol to 5L</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Buffers]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Buffers]]</div></td></tr> <!-- diff generator: internal 2013-05-22 17:57:19 --> </table> Wed, 06 May 2009 18:04:19 GMT Nessie Van Loan http://openwetware.org/wiki/Talk:CAPS Nessie Van Loan: /* Recipe */ removed superfluous and http://openwetware.org/index.php?title=CAPS&diff=303616&oldid=prev <p><span class="autocomment">Recipe:&#32;</span> removed superfluous and</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:49, 21 April 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 12:</td> <td colspan="2" class="diff-lineno">Line 12:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 10% Methanol to 5L</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 10% Methanol to 5L</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Category:Material]] <del class="diffchange diffchange-inline">and </del>[[Category:Buffers]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Buffers]]</div></td></tr> <!-- diff generator: internal 2013-05-22 17:57:19 --> </table> Tue, 21 Apr 2009 23:49:12 GMT Nessie Van Loan http://openwetware.org/wiki/Talk:CAPS Nessie Van Loan: Forgot some things,. http://openwetware.org/index.php?title=CAPS&diff=303614&oldid=prev <p>Forgot some things,.</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:46, 21 April 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots<ins class="diffchange diffchange-inline">.&nbsp; The powder has a formula weight of 221.32g/mol.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Recipe==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Recipe==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 5L of 1X CAPS,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 5L of 1X CAPS,</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Combine the following:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Combine the following:</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#* 11.<del class="diffchange diffchange-inline">07g </del>CAPS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#* 11.<ins class="diffchange diffchange-inline">066g </ins>CAPS</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 4.5L 10% Methanol</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#* 4.5L 10% Methanol</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Adjust pH to 7.4 with around 5mL concentrated HCl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Adjust pH to 7.4 with around 5mL concentrated HCl</div></td></tr> <!-- diff generator: internal 2013-05-22 17:57:19 --> </table> Tue, 21 Apr 2009 23:46:27 GMT Nessie Van Loan http://openwetware.org/wiki/Talk:CAPS Nessie Van Loan: Created page. Has to ask what this was, and decided to share the answer http://openwetware.org/index.php?title=CAPS&diff=303613&oldid=prev <p>Created page. Has to ask what this was, and decided to share the answer</p> <p><b>New page</b></p><div>==Purpose==<br /> CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots<br /> <br /> ==Recipe==<br /> 1X CAPS is 10mM, pH 11<br /> <br /> To make 5L of 1X CAPS,<br /> # Combine the following:<br /> #* 11.07g CAPS<br /> #* 4.5L 10% Methanol<br /> # Adjust pH to 7.4 with around 5mL concentrated HCl<br /> # Add 10% Methanol to 5L<br /> <br /> [[Category:Material]] and [[Category:Buffers]]</div> Tue, 21 Apr 2009 23:43:16 GMT Nessie Van Loan http://openwetware.org/wiki/Talk:CAPS