CAPS
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m (Forgot some things,.) |
Current revision (14:04, 6 May 2009) (view source) (had conflicting pH's, fixed) |
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#* 11.066g CAPS | #* 11.066g CAPS | ||
#* 4.5L 10% Methanol | #* 4.5L 10% Methanol | ||
| - | # Adjust pH to | + | # Adjust pH to 11 with around 5mL 10N NaOH |
# Add 10% Methanol to 5L | # Add 10% Methanol to 5L | ||
| - | [[Category:Material]] | + | [[Category:Material]] [[Category:Buffers]] |
Current revision
Purpose
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.
Recipe
1X CAPS is 10mM, pH 11
To make 5L of 1X CAPS,
- Combine the following:
- 11.066g CAPS
- 4.5L 10% Methanol
- Adjust pH to 11 with around 5mL 10N NaOH
- Add 10% Methanol to 5L
