CAPS

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Current revision (14:04, 6 May 2009) (view source)
(had conflicting pH's, fixed)
 
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==Purpose==
==Purpose==
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CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots
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CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots.  The powder has a formula weight of 221.32g/mol.
==Recipe==
==Recipe==
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To make 5L of 1X CAPS,
To make 5L of 1X CAPS,
# Combine the following:
# Combine the following:
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#* 11.07g CAPS
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#* 11.066g CAPS
#* 4.5L 10% Methanol
#* 4.5L 10% Methanol
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# Adjust pH to 7.4 with around 5mL concentrated HCl
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# Adjust pH to 11 with around 5mL 10N NaOH
# Add 10% Methanol to 5L
# Add 10% Methanol to 5L
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[[Category:Material]] and [[Category:Buffers]]
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[[Category:Material]] [[Category:Buffers]]

Current revision

Purpose

CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.

Recipe

1X CAPS is 10mM, pH 11

To make 5L of 1X CAPS,

  1. Combine the following:
    • 11.066g CAPS
    • 4.5L 10% Methanol
  2. Adjust pH to 11 with around 5mL 10N NaOH
  3. Add 10% Methanol to 5L
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