CAPS: Difference between revisions
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==Purpose== | ==Purpose== | ||
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots | CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol. | ||
==Recipe== | ==Recipe== | ||
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To make 5L of 1X CAPS, | To make 5L of 1X CAPS, | ||
# Combine the following: | # Combine the following: | ||
#* 11. | #* 11.066g CAPS | ||
#* 4.5L 10% Methanol | #* 4.5L 10% Methanol | ||
# Adjust pH to 7.4 with around 5mL concentrated HCl | # Adjust pH to 7.4 with around 5mL concentrated HCl | ||
Revision as of 16:46, 21 April 2009
Purpose
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.
Recipe
1X CAPS is 10mM, pH 11
To make 5L of 1X CAPS,
- Combine the following:
- 11.066g CAPS
- 4.5L 10% Methanol
- Adjust pH to 7.4 with around 5mL concentrated HCl
- Add 10% Methanol to 5L and
