CAPS

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(Created page. Has to ask what this was, and decided to share the answer)
m (Forgot some things,.)
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==Purpose==
==Purpose==
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CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots
+
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots.  The powder has a formula weight of 221.32g/mol.
==Recipe==
==Recipe==
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To make 5L of 1X CAPS,
To make 5L of 1X CAPS,
# Combine the following:
# Combine the following:
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#* 11.07g CAPS
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#* 11.066g CAPS
#* 4.5L 10% Methanol
#* 4.5L 10% Methanol
# Adjust pH to 7.4 with around 5mL concentrated HCl
# Adjust pH to 7.4 with around 5mL concentrated HCl

Revision as of 18:46, 21 April 2009

Purpose

CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.

Recipe

1X CAPS is 10mM, pH 11

To make 5L of 1X CAPS,

  1. Combine the following:
    • 11.066g CAPS
    • 4.5L 10% Methanol
  2. Adjust pH to 7.4 with around 5mL concentrated HCl
  3. Add 10% Methanol to 5L and
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