Build-a-Gene Session 4: Difference between revisions
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2. Incubate at 16C for 30 min | |||
3. Incubate at 80C for 20 min | 3. Incubate at 80C for 20 min | ||
Revision as of 07:40, 12 August 2013
LIGATION
This step will allow us to join the vector, the promoter, and the emGFP gene together into one circular DNA molecule.
1. Combine all of the reagents below together into one thin-walled PCR tube.
| Vector | 1,5 ul |
| emGFP gene | 1.5 ul |
| Promoter | 1.5 ul |
| T4 Ligase + buffer mix | 5.5 ul |
| Total | 10 ul |
2. Incubate at 16C for 30 min
3. Incubate at 80C for 20 min
