Bromophenol blue - Revision history

Jakob Suckale at 14:18, 3 June 2009

Jakob Suckale — Wed, 03 Jun 2009 14:18:02 GMT

←Older revision		Revision as of 14:18, 3 June 2009
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	Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.		Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

-	* 500bp at 1% ~~agarose~~	+	* 1000bp at 0.7% agarose
		+	* 500bp at 1%
	* 150bp at 2%		* 150bp at 2%
	* 50bp at 3%		* 50bp at 3%

Jakob Suckale: illustration

Jakob Suckale — Wed, 03 Jun 2009 14:17:30 GMT

illustration

←Older revision		Revision as of 14:17, 3 June 2009
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		+	[[Image:Agarose gel dyes.jpg\|right\|200px]]
		+
	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).		'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).

Jakob Suckale at 15:29, 5 May 2009

Jakob Suckale — Tue, 05 May 2009 15:29:07 GMT

←Older revision		Revision as of 15:29, 5 May 2009
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	* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]		* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]

-	[[Category:Material]] [[Category:~~Chemical~~]] [[Category:Agarose gel electrophoresis]]	+	[[Category:Material]] [[Category:Dye]] [[Category:Agarose gel electrophoresis]]

Jakob Suckale at 15:28, 5 May 2009

Jakob Suckale — Tue, 05 May 2009 15:28:44 GMT

←Older revision		Revision as of 15:28, 5 May 2009
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-	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (~~hydyroxyl~~ groups).	+	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups).

	Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.		Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

-	* 500bp at 1% ~~agaros~~	+	* 500bp at 1% agarose
	* 150bp at 2%		* 150bp at 2%
	* 50bp at 3%		* 50bp at 3%

Jakob Suckale: size

Jakob Suckale — Tue, 05 May 2009 15:28:29 GMT

size

←Older revision		Revision as of 15:28, 5 May 2009
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	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydyroxyl groups).		'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydyroxyl groups).
		+
		+	Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
		+
		+	* 500bp at 1% agaros
		+	* 150bp at 2%
		+	* 50bp at 3%

	== See also ==		== See also ==

Jakob Suckale: categories

Jakob Suckale — Tue, 05 May 2009 15:18:41 GMT

Jakob Suckale at 15:18, 5 May 2009

Jakob Suckale — Tue, 05 May 2009 15:18:02 GMT

←Older revision		Revision as of 15:18, 5 May 2009
Line 1:		Line 1:
-	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of ~~1 net~~ negative charge.	+	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydyroxyl groups).

	== See also ==		== See also ==

Jakob Suckale: links

Jakob Suckale — Tue, 05 May 2009 15:10:05 GMT

links

←Older revision		Revision as of 15:10, 5 May 2009
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-	'''Bromophenol blue''' is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.	+	'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.
		+
		+	== See also ==
		+	* [[agarose gel loading buffer]]
		+	* [[agarose gel electrophoresis]]
		+	* [[Xylene cyanol]] - slower than BPB
		+	* [[Cresol Red]] - slower than BPB
		+
		+	== External links ==
		+	* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]

Jakob Suckale: lead section

Jakob Suckale — Tue, 05 May 2009 15:08:09 GMT

lead section

New page

'''Bromophenol blue''' is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.

←Older revision		Revision as of 15:18, 5 May 2009
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	== External links ==		== External links ==
	* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]		* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]
		+
		+	[[Category:Material]] [[Category:Chemical]] [[Category:Agarose gel electrophoresis]]