Bromophenol blue: Difference between revisions
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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge ( | '''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups). | ||
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH. | Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH. | ||
* 500bp at 1% | * 500bp at 1% agarose | ||
* 150bp at 2% | * 150bp at 2% | ||
* 50bp at 3% | * 50bp at 3% | ||
Revision as of 08:28, 5 May 2009
Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
- 500bp at 1% agarose
- 150bp at 2%
- 50bp at 3%
See also
- agarose gel loading buffer
- agarose gel electrophoresis
- Xylene cyanol - slower than BPB
- Cresol Red - slower than BPB
