Bromophenol blue: Difference between revisions
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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge ( | [[Image:Agarose gel dyes.jpg|right|200px]] | ||
'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydroxyl groups). | |||
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH. | |||
* 1000bp at 0.7% agarose | |||
* 500bp at 1% | |||
* 150bp at 2% | |||
* 50bp at 3% | |||
== See also == | == See also == | ||
| Line 10: | Line 19: | ||
* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia] | * [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia] | ||
[[Category:Material]] [[Category: | [[Category:Material]] [[Category:Dye]] [[Category:Agarose gel electrophoresis]] | ||
Latest revision as of 07:18, 3 June 2009
Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
- 1000bp at 0.7% agarose
- 500bp at 1%
- 150bp at 2%
- 50bp at 3%
See also
- agarose gel loading buffer
- agarose gel electrophoresis
- Xylene cyanol - slower than BPB
- Cresol Red - slower than BPB
