Bromophenol blue

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
m
Line 16: Line 16:
* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]
* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]
-
[[Category:Material]] [[Category:Chemical]] [[Category:Agarose gel electrophoresis]]
+
[[Category:Material]] [[Category:Dye]] [[Category:Agarose gel electrophoresis]]

Revision as of 10:29, 5 May 2009

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).

Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

  • 500bp at 1% agarose
  • 150bp at 2%
  • 50bp at 3%

See also

External links

Personal tools