Bromophenol blue

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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydyroxyl groups).
'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] (539g/mol) because of its larger negative charge (hydyroxyl groups).
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Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
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* 500bp at 1% agaros
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* 150bp at 2%
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* 50bp at 3%
== See also ==
== See also ==

Revision as of 11:28, 5 May 2009

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydyroxyl groups).

Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

  • 500bp at 1% agaros
  • 150bp at 2%
  • 50bp at 3%

See also

External links

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