Bromophenol blue

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'''Bromophenol blue''' is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.
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'''Bromophenol blue''' (BPB) is most commonly used as an [[agarose gel electrophoresis]] size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than [[Xylene cyanol]] because of 1 net negative charge.
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== See also ==
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* [[agarose gel loading buffer]]
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* [[agarose gel electrophoresis]]
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* [[Xylene cyanol]] - slower than BPB
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* [[Cresol Red]] - slower than BPB
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== External links ==
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* [http://en.wikipedia.org/wiki/Bromophenol_blue Bromophenol blue at the Wikipedia]

Revision as of 10:10, 5 May 2009

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol because of 1 net negative charge.

See also

External links

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