Bromodeoxyuridine (BrdU)

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[[Image:BrdU vs dT.svg|thumb|right|250px|'''BrdU''' next to the deoxynucleoside '''deoxythymidine''' (dT) it can replace during DNA synthesis]]
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'''Bromodeoxyuridine''' (5-bromo-2-deoxyuridine, '''BrdU''') is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation.
'''Bromodeoxyuridine''' (5-bromo-2-deoxyuridine, '''BrdU''') is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation.
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== Mechanism ==
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BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.
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== Examples ==
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* [[User:Mar/Notebook/2007-9-20|BrdU application in E16 mouse embryos]]
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== See also ==
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* [[20.109(S08):Testing cell viability (Day3)|Fundamental bioengineering course notes]] mention BrdU in the introduction
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* [http://en.wikipedia.org/wiki/BrdU bromodeoxyuridine (BrdU)], [http://en.wikipedia.org/wiki/Deoxyuridine deoxyuridine (dU)], [http://en.wikipedia.org/wiki/Deoxythymidine deoxythymidine (dT)] at the Wikipedia
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[[Category:Material]]
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[[Category:DNA]]
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[[Category:Chemical]]

Current revision

BrdU next to the deoxynucleoside deoxythymidine (dT) it can replace during DNA synthesis
BrdU next to the deoxynucleoside deoxythymidine (dT) it can replace during DNA synthesis

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation.

Mechanism

BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.

Examples

See also

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