Biomolecular Breadboards:pBEST OR2 OR1 Pr UTR-deGFP-T500.gb: Difference between revisions

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* Control sequence: [[Media:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb]] (GenBank file)
* Control sequence: [[Media:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb]] (GenBank file)
* Preliminary data: [[Media:plasmid_deGFP-070812.xls|plasmid_deGFP-070812.xls]] (Excel spreadsheet)
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=== Plasmid description ===
=== Preliminary data ===
Using pBEST-OR2-OR1-Pr-UTR1-deGFP-T500, a plasmid enhanced for GFP expression, the biomolecular breadboard is able to express mass at equal concentrations to comparable bacteriophage in-vitro systems (J. Shin and V. Noireaux, 2010).
Expression of plasmids can be optimized by concentration.
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[[Image:plasmid_sat.png|400px]]
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* Raw data file: [[Media:plasmid_deGFP-070812.xls|plasmid_deGFP-070812.xls]] (Excel spreadsheet)
* Information about protocol on "Outline" sheet; plotted data is on the sheet "plotted-data"
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* Control plasmid file: [[Media:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb]] (GenBank format)
* More information: [[Biomolecular Breadboards:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|Control plasmid description]]
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'''Figure 1. eGFP expression as a function of plasmid DNA template.''' Plasmid DNA pBEST-OR2-OR1-Pr-UTR1-eGFP-Del6-229-T500 is varied by concentration.

Revision as of 20:34, 16 July 2012

This page contains a description of the deGFP control plasmid for the cell-free expression breadboard.

Plasmid description

Preliminary data

Using pBEST-OR2-OR1-Pr-UTR1-deGFP-T500, a plasmid enhanced for GFP expression, the biomolecular breadboard is able to express mass at equal concentrations to comparable bacteriophage in-vitro systems (J. Shin and V. Noireaux, 2010).

Expression of plasmids can be optimized by concentration.

  • Raw data file: plasmid_deGFP-070812.xls (Excel spreadsheet)
  • Information about protocol on "Outline" sheet; plotted data is on the sheet "plotted-data"


Figure 1. eGFP expression as a function of plasmid DNA template. Plasmid DNA pBEST-OR2-OR1-Pr-UTR1-eGFP-Del6-229-T500 is varied by concentration.

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