Biomolecular Breadboards:Protocols:cost estimate: Difference between revisions

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| style="width: 200px;" | '''Total Price of ''E. coli'' crude extract, 3x Prep'''                  (Ingredients + Disposable Materials) || style="width: 100px;" | $141.10
| style="width: 200px;" | '''Total Price of ''E. coli'' crude extract, 3x Prep'''                  (Ingredients + Disposable Materials) || style="width: 100px;" | $141.10
|-
|-
| '''Price per uL of reaction''' || $0.00448
| '''Price per uL of reaction''' || '''$0.00448'''
|-
|-
| '''Price per 12 uL reaction''' || $0.05376
| '''Price per 12 uL reaction''' || '''$0.05376'''
|}
|}


===3-PGA===
 
 
 
===Amino Acids===
One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 cell-free circuit reactions.  See [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] for instructions on kit preparation.
 
{| border="1" cellpadding="5" style="width: 1050px"
! scope="col" | Ingredient
! scope="col" | Quantity
! scope="col" | Price
! scope="col" | '''Price per uL of reaction'''
! scope="col" | '''Price per 12 uL reaction'''
! scope="col" | Source
! scope="col" | Catalog Number
 
|-
| style="width: 250px;" | RTS Amino Acid Sampler || style="width: 100px;" | 1 kit || style="width: 100px;" | $349.00 || style="width: 100px;" | '''$0.0021''' || style="width: 100px;" | '''$0.0252''' || style="width: 200px;" | 5 Prime || style="width: 200 px;" | 2401530
|}

Revision as of 19:16, 11 August 2012

Cell-Free Circuit Breadboard Cost Estimate

Reaction

E. coli crude extract

"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol E. Coli Crude Extract Preparation, 3X. On average, one prep yields 10.5 mL of extract, which is enough for about 31500 cell-free circuit reactions.

Ingredient Bulk Quantity Bulk Price Amount per 3X prep Price per 3X prep Source Catalog Number
2xYT 1 kg $87.3 139.93 g $12.216 MP biomedicals 3012-032
Bacto-Agar 1 lb $171.11 0.44 g $0.17 BD (from Fisher) 214010
Chloramphenicol powder 5 g $30.50 3.876 mg $0.02 Sigma-Aldrich C1919
DTT 5 g $97.40 0.927 g $18.06 Sigma-Aldrich D0632
Ethanol (100%) 500 mL $54.68 114 uL $0.01 Fisher BP2818
K-glutamate 500 g $161.50 48.8 g $15.76 Sigma-Aldrich G1149
Mg-glutamate 250 g $47.10 21.76 g $4.10 Sigma-Aldrich 49605
Potassium phosphate monobasic solution 1 L $43.80 90.15 mL $3.95 Sigma-Aldrich P8709
Potassium phosphate dibasic solution 1 L $38.00 163.92 mL $6.23 Sigma-Aldrich P8584
Tris 1 kg $133.58 13.325 g $1.78 Fisher BP1521
Total Price $62.30



Disposable Material Bulk Quantity Bulk Price Amount per 3X prep Price per 3X prep Source Catalog Number
2xYT 1 kg $87.3 139.93 g $12.216 MP biomedicals 3012-032
Beads (0.1 mm diameter) 1 lb $34.00 80 g $6.00 BioSpec 11079101
Bead beating tubes 500 count $141.00 42 count $11.84 BioSpec 522S
Bead filters 100 count $112.00 6 count $6.72 Bio-Rad 732-6204
Bio-rad Bradford protein assay kit (BSA) 1 kit $123.00 1 assay $0.62 Bio-Rad 500-0201
Cuvettes 500 count $170.30 7 count $2.38 Fisher 14-955-127
Dialysis cassettes 10 count $85.40 6 count $51.24 Thermo Scientific (from Fisher) 66380
Total Price $78.80



Total Price of E. coli crude extract, 3x Prep (Ingredients + Disposable Materials) $141.10
Price per uL of reaction $0.00448
Price per 12 uL reaction $0.05376



Amino Acids

One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 cell-free circuit reactions. See Amino Acid Preparation for instructions on kit preparation.

Ingredient Quantity Price Price per uL of reaction Price per 12 uL reaction Source Catalog Number
RTS Amino Acid Sampler 1 kit $349.00 $0.0021 $0.0252 5 Prime 2401530
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