Biomolecular Breadboards:Protocols:cost estimate: Difference between revisions
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|Tris || 1 kg || $133.58 || 13.325 g || $1.78 || Fisher || BP1521 | |Tris || 1 kg || $133.58 || 13.325 g || $1.78 || Fisher || BP1521 | ||
|- | |||
|'''Total Price''' || || '''$864.97''' || ||'''$62.30''' || || | |||
|} | |||
{| border="1" cellpadding="5" style="width: 1050px" | |||
! scope="col" | Disposable Materials | |||
! scope="col" | Bulk Quantity | |||
! scope="col" | Bulk Price | |||
! scope="col" | Amount per 3X prep | |||
! scope="col" | Price per 3X prep | |||
! scope="col" | Source | |||
! scope="col" | Catalog Number | |||
|- | |||
| style="width: 250px;" | 2xYT || style="width: 100px;" | 1 kg || style="width: 100px;" | $87.3|| style="width: 100px;" | 139.93 g || style="width: 100px;" | $12.216 || style="width: 200px;" | MP biomedicals || style="width: 200 px;" | 3012-032 | |||
|- | |||
| Beads (0.1 mm diameter) || 1 lb || $34.00 || 80 g || $6.00 || BioSpec || 11079101 | |||
|- | |||
| Bead beating tubes || 500 count || $141.00 || 42 count || $11.84 || BioSpec || 522S | |||
|- | |||
| Bead filters || 100 count || $112.00 || 6 count || $6.72 || Bio-Rad || 732-6204 | |||
|- | |||
| Bio-rad Bradford protein assay kit (BSA) || 1 kit || $123.00 || 1 assay || $0.62 || Bio-Rad || 500-0201 | |||
|- | |||
|Cuvettes || 500 count || $170.30 || 7 count || $2.38 || Fisher || 14-955-127 | |||
|- | |||
|Dialysis cassettes || 10 count || $85.40 || 6 count || $51.24 || Thermo Scientific (from Fisher) || 66380 | |||
|- | |||
| '''Total Price''' || || '''$665.70''' || || '''$78.80''' || || | |||
|} | |} | ||
===Amino acids=== | ===Amino acids=== | ||
===3-PGA=== | ===3-PGA=== | ||
Revision as of 18:15, 11 August 2012
Cell-Free Circuit Breadboard Cost Estimate
Reaction
E. coli crude extract
"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol E. Coli Crude Extract Preparation, 3X .
| Ingredient | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Bacto-Agar | 1 lb | $171.11 | 0.44 g | $0.17 | BD (from Fisher) | 214010 |
| Chloramphenicol powder | 5 g | $30.50 | 3.876 mg | $0.02 | Sigma-Aldrich | C1919 |
| DTT | 5 g | $97.40 | 0.927 g | $18.06 | Sigma-Aldrich | D0632 |
| Ethanol (100%) | 500 mL | $54.68 | 114 uL | $0.01 | Fisher | BP2818 |
| K-glutamate | 500 g | $161.50 | 48.8 g | $15.76 | Sigma-Aldrich | G1149 |
| Mg-glutamate | 250 g | $47.10 | 21.76 g | $4.10 | Sigma-Aldrich | 49605 |
| Potassium phosphate monobasic solution | 1 L | $43.80 | 90.15 mL | $3.95 | Sigma-Aldrich | P8709 |
| Potassium phosphate dibasic solution | 1 L | $38.00 | 163.92 mL | $6.23 | Sigma-Aldrich | P8584 |
| Tris | 1 kg | $133.58 | 13.325 g | $1.78 | Fisher | BP1521 |
| Total Price | $864.97 | $62.30 |
| Disposable Materials | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Beads (0.1 mm diameter) | 1 lb | $34.00 | 80 g | $6.00 | BioSpec | 11079101 |
| Bead beating tubes | 500 count | $141.00 | 42 count | $11.84 | BioSpec | 522S |
| Bead filters | 100 count | $112.00 | 6 count | $6.72 | Bio-Rad | 732-6204 |
| Bio-rad Bradford protein assay kit (BSA) | 1 kit | $123.00 | 1 assay | $0.62 | Bio-Rad | 500-0201 |
| Cuvettes | 500 count | $170.30 | 7 count | $2.38 | Fisher | 14-955-127 |
| Dialysis cassettes | 10 count | $85.40 | 6 count | $51.24 | Thermo Scientific (from Fisher) | 66380 |
| Total Price | $665.70 | $78.80 |
