Biomolecular Breadboards:Protocols:cost estimate: Difference between revisions
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"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol [[Biomolecular Breadboards:Protocols:Crude Extract Prep|''E. Coli'' Crude Extract Preparation, 3X]] . | "Amount per 3X prep" refers to the amount of crude extract you would make following the protocol [[Biomolecular Breadboards:Protocols:Crude Extract Prep|''E. Coli'' Crude Extract Preparation, 3X]] . | ||
{| border="1" cellpadding="5" style="width: | {| border="1" cellpadding="5" style="width: 1050px" | ||
! scope="col" | Ingredient | ! scope="col" | Ingredient | ||
! scope="col" | Bulk Quantity | ! scope="col" | Bulk Quantity | ||
| Line 16: | Line 16: | ||
|- | |- | ||
| style="width: | | style="width: 250px;" | 2xYT || style="width: 100px;" | 1 kg || style="width: 100px;" | $87.3|| style="width: 100px;" | 139.93 g || style="width: 100px;" | $12.216 || style="width: 200px;" | MP biomedicals || style="width: 200 px;" | 3012-032 | ||
|- | |- | ||
| Bacto-Agar || 1 lb || $171.11 || 0.44 g || $0.17 || BD (from Fisher) || 214010 | |||
|- | |||
| Chloramphenicol powder || 5 g || $30.50 || 3.876 mg || $0.02 || Sigma-Aldrich || C1919 | |||
|- | |||
| DTT || 5 g || $97.40 || 0.927 g || $18.06 || Sigma-Aldrich || D0632 | |||
|- | |||
| Ethanol (100%) || 500 mL || $54.68 || 114 uL || $0.01 || Fisher || BP2818 | |||
|- | |||
|K-glutamate || 500 g || $161.50 || 48.8 g || $15.76 || Sigma-Aldrich || G1149 | |||
|- | |||
|Mg-glutamate || 250 g || $47.10 || 21.76 g || $4.10 || Sigma-Aldrich || 49605 | |||
|- | |||
|Potassium phosphate monobasic solution || 1 L || $43.80 || 90.15 mL || $3.95 || Sigma-Aldrich || P8709 | |||
|- | |||
|Potassium phosphate dibasic solution || 1 L || $38.00 || 163.92 mL || $6.23 || Sigma-Aldrich || P8584 | |||
|- | |||
|Tris || 1 kg || $133.58 || 13.325 g || $1.78 || Fisher || BP1521 | |||
|} | |} | ||
===Amino acids=== | ===Amino acids=== | ||
===3-PGA=== | ===3-PGA=== | ||
Revision as of 17:57, 11 August 2012
Cell-Free Circuit Breadboard Cost Estimate
Reaction
E. coli crude extract
"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol E. Coli Crude Extract Preparation, 3X .
| Ingredient | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Bacto-Agar | 1 lb | $171.11 | 0.44 g | $0.17 | BD (from Fisher) | 214010 |
| Chloramphenicol powder | 5 g | $30.50 | 3.876 mg | $0.02 | Sigma-Aldrich | C1919 |
| DTT | 5 g | $97.40 | 0.927 g | $18.06 | Sigma-Aldrich | D0632 |
| Ethanol (100%) | 500 mL | $54.68 | 114 uL | $0.01 | Fisher | BP2818 |
| K-glutamate | 500 g | $161.50 | 48.8 g | $15.76 | Sigma-Aldrich | G1149 |
| Mg-glutamate | 250 g | $47.10 | 21.76 g | $4.10 | Sigma-Aldrich | 49605 |
| Potassium phosphate monobasic solution | 1 L | $43.80 | 90.15 mL | $3.95 | Sigma-Aldrich | P8709 |
| Potassium phosphate dibasic solution | 1 L | $38.00 | 163.92 mL | $6.23 | Sigma-Aldrich | P8584 |
| Tris | 1 kg | $133.58 | 13.325 g | $1.78 | Fisher | BP1521 |
