Biomolecular Breadboards:Protocols

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(Design Cycle Time Analysis)
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{| border = "1" cellpadding="5" style="width: 800px"
{| border = "1" cellpadding="5" style="width: 800px"
! scope="col" | Simple circuit
! scope="col" | Simple circuit
-
! scope="col" | Assembly time for ''in vitro'' testing
+
! scope="col" | Assembly time for ''in vitro'' testing (linear DNA)
 +
! scope="col" | Assembly time for ''in vitro'' testing (multiple plasmids)
 +
! scope="col" | Assembly time for ''in vitro'' testing (single plasmid)
! scope="col" | Assembly time for ''in vivo'' testing  
! scope="col" | Assembly time for ''in vivo'' testing  
(1 plasmid construct)
(1 plasmid construct)
|-
|-
-
| style="width: 200px; | Negatively autoregulated gene || style="text-align:center" | 6.5 hours || style="text-align:center" | 3 days
+
| style="width: 200px; | Negatively autoregulated gene  
 +
| style="text-align:center" | 6.5 hours  
 +
| style="text-align:center" | N/A
 +
| style="text-align:center" | 3 days
 +
| style="text-align:center" | 3 days
|-
|-
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| Bistable switch || style="width: 200px; text-align:center" | 3 days || style="width: 200px; text-align:center"  | 5 days minimum*
+
| Bistable switch  
 +
| style="width: 200px; text-align:center" | 6.5 hours
 +
| style="width: 200px; text-align:center" | 3 days  
 +
| style="width: 200px; text-align:center" | 5+ days*
 +
| style="width: 200px; text-align:center"  | 5+ days*
|}
|}

Revision as of 11:56, 20 November 2012

Home Protocols DNA parts Preliminary Data Models More Info

Cell-free circuit breadboard

The following protocols are available for the cell-free circuit breadboard:

Cost estimate

The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.

Component Material price per

μL of reaction

Labor price per

μL of reaction

Total price per

μL of reaction

Total price per

12 μL reaction

E. coli crude extract 0.45 cents 0.62 cents 1.07 cents 12.84 cents
Amino acids 0.21 cents 0.03 cents 0.24 cents 2.93 cents
3-PGA 0.39 cents 0.94 cents 1.33 cents 15.96 cents
Total price 1.05 cents 1.6 cents 2.64 cents 31.7 cents

For a more detailed cost estimate, please see Breadboard Cost Estimate.

Design Cycle Time Analysis

Simple circuit Assembly time for in vitro testing (linear DNA) Assembly time for in vitro testing (multiple plasmids) Assembly time for in vitro testing (single plasmid) Assembly time for in vivo testing

(1 plasmid construct)

Negatively autoregulated gene 6.5 hours N/A 3 days 3 days
Bistable switch 6.5 hours 3 days 5+ days* 5+ days*

( * Estimated. All other times are measured. )


For a more detailed description, please see Breadboard Cycle Time Measurements.

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