Biomolecular Breadboards:Protocols: Difference between revisions
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** [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] | ** [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] | ||
** [[Biomolecular Breadboards:Protocols:3-PGA Buffer Prep|3-PGA Buffer Preparation, 14X]] | ** [[Biomolecular Breadboards:Protocols:3-PGA Buffer Prep|3-PGA Buffer Preparation, 14X]] | ||
* [[Media: TXTL_v2_3. | * [[Media: TXTL_v2_3.xlsx | TXTL template, v2.3]] - TXTL template for designing experiments | ||
=== Cost estimate === | === Cost estimate === | ||
Revision as of 08:17, 13 August 2012
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Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
- TXTL template, v2.3 - TXTL template for designing experiments
Cost estimate
The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.
| Component | Material price per
μL of reaction |
Labor price per
μL of reaction |
Total price per
μL of reaction |
Total price per
12 μL reaction |
|---|---|---|---|---|
| E. coli crude extract | 0.45 cents | 0.62 cents | 1.07 cents | 12.84 cents |
| Amino acids | 0.21 cents | 0.03 cents | 0.24 cents | 2.93 cents |
| 3-PGA | 0.39 cents | 0.94 cents | 1.33 cents | 15.96 cents |
| Total price | 1.05 cents | 1.6 cents | 2.64 cents | 31.7 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
