Biomolecular Breadboards:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Clare Hayes (talk | contribs) mNo edit summary |
Clare Hayes (talk | contribs) mNo edit summary |
||
| Line 7: | Line 7: | ||
** [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] | ** [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] | ||
** [[Biomolecular Breadboards:Protocols:3-PGA Buffer Prep|3-PGA Buffer Preparation, 14X]] | ** [[Biomolecular Breadboards:Protocols:3-PGA Buffer Prep|3-PGA Buffer Preparation, 14X]] | ||
=== Cost estimate === | === Cost estimate === | ||
The cell-free circuit breadboard costs approximately 1.05 cents per uL, or 12.6 cents per typical 12 uL reaction. The cost distribution is shown in the table below. | |||
{| border="1" cellpadding="5" style="width: 400px" | {| border="1" cellpadding="5" style="width: 400px" | ||
! scope="col" | Component | ! scope="col" | Component | ||
| Line 24: | Line 25: | ||
| '''Total price''' || style="text-align:center" | 1.05 cents || style="text-align:center" | 12.6 cents | | '''Total price''' || style="text-align:center" | 1.05 cents || style="text-align:center" | 12.6 cents | ||
|} | |} | ||
For a more detailed cost estimate, please see [[Biomolecular Breadboards:Protocols:cost_estimate|Breadboard Cost Estimate]]. | |||
Revision as of 21:33, 11 August 2012
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
Cost estimate
The cell-free circuit breadboard costs approximately 1.05 cents per uL, or 12.6 cents per typical 12 uL reaction. The cost distribution is shown in the table below.
| Component | Price per μL of reaction | Price per typical 12 μL reaction |
|---|---|---|
| E. coli crude extract | 0.45 cents | 5.4 cents |
| Amino acids | 0.21 cents | 2.5 cents |
| 3-PGA | 0.39 cents | 4.7 cents |
| Total price | 1.05 cents | 12.6 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
