Biomod/2015/OhioMOD: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(138 intermediate revisions by the same user not shown)
Line 1: Line 1:
<html>
<html>
<head>
<style>
<style>
 
#content{margin-left:0; margin-top:0; margin-bottom:0;}
.navbar{
top:0px;
position:fixed;
width:96.8%;
}
/*clearing the side openwetware panels*/
/*clearing the side openwetware panels*/
.firstHeading, #column-one,  #p-bookmarks, #p-history , .portlet{
.firstHeading, #column-one,  #p-bookmarks, #p-history , .portlet{
display:none; border-style: transparent;
display:none;
}
}
#bodyContent/*clearing the side openwetware panels
#bodyContent/*clearing the side openwetware panels
Line 16: Line 19:
background-color:#FFFFFF;
background-color:#FFFFFF;
}
}
 
#OSUfooter{
</style>
clear:both;
 
position:relative;
</head>
}
 
#footer{display: none;}
<body>
<body>
<!--css stuff begins here-->
<!--css stuff begins here-->
<style>
<style>
{
 
color: #000016;
background-color: #BDE20D;
}


.html
.html
Line 35: Line 36:
}
}


#page
{
display:block;
z-index: 1;
width: 792px;
height: 700px;
background-image: none;
border-style: none;
border-color: #000000;
background-color: transparent;
margin-left: auto;
margin-right: auto;
padding: 2px 99px 62px 69px;
}


#u450
#u450
{
{
z-index: 2;
 
width: 792px;
width: 792px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-left: 325px;
}
}


Line 65: Line 53:
#u456
#u456
{
{
z-index: 4;
width: 99px;
width: 99px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
}
}


#u468
#u468
{
{
z-index: 6;
 
width: 574px;
width: 574px;
position: relative;
position: relative;
background-color: transparent;
background-color: transparent;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
left: 218px;
left: 218px;
top:-552px;
top:-552px;
Line 86: Line 75:
#u474
#u474
{
{
z-index: 8;
 
width: 131px;
width: 131px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 1px;
margin-top: 1px;
left: 218px;
left: 218px;
Line 98: Line 88:
#u486
#u486
{
{
z-index: 10;
 
width: 317px;
width: 317px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 1px;
margin-top: 1px;
left: 475px;
left: 475px;
Line 110: Line 101:
#u492
#u492
{
{
z-index: 12;
 
width: 95px;
width: 95px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 12px;
margin-top: 12px;
left: 475px;
left: 475px;
Line 122: Line 114:
#u504
#u504
{
{
z-index: 14;
 
width: 71px;
width: 71px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 12px;
margin-top: 12px;
left: 721px;
left: 721px;
Line 134: Line 127:
#u510
#u510
{
{
z-index: 16;
width: 126px;
width: 126px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 136px;
margin-top: 136px;
left: 349px;
left: 349px;
Line 146: Line 139:
#u516
#u516
{
{
z-index: 18;
 
width: 151px;
width: 151px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 148px;
margin-top: 148px;
left: 570px;
left: 570px;
Line 158: Line 152:
#u522
#u522
{
{
z-index: 20;
 
width: 119px;
width: 119px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 153px;
margin-top: 153px;
left: 99px;
left: 99px;
Line 170: Line 165:
#u528
#u528
{
{
z-index: 22;
 
width: 81px;
width: 81px;
background-color: transparent;
background-color: transparent;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 158px;
margin-top: 158px;
left: 99px;
left: 99px;
Line 182: Line 178:
#u534
#u534
{
{
z-index: 24;
 
width: 464px;
width: 464px;
background-color: transparent;
background-color: transparent;
Line 188: Line 184:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 158px;
margin-top: 158px;
margin-left: 325px;
left: 180px;
left: 180px;
top:-2459px;
top:-2459px;
Line 194: Line 191:
#u540
#u540
{
{
z-index: 26;
 
width: 77px;
width: 77px;
background-color: transparent;
background-color: transparent;
Line 200: Line 197:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 158px;
margin-top: 158px;
margin-left: 325px;
left: 644px;
left: 644px;
top:-2705px;
top:-2705px;
Line 206: Line 204:
#u546
#u546
{
{
z-index: 28;
 
width: 464px;
width: 464px;
background-color: transparent;
background-color: transparent;
Line 212: Line 210:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 246px;
margin-top: 246px;
margin-left: 325px;
left: 180px;
left: 180px;
top:-3013px;
top:-3013px;
Line 218: Line 217:
#u552
#u552
{
{
z-index: 30;
width: 195px;
width: 195px;
background-color: transparent;
background-color: transparent;
Line 224: Line 222:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 270px;
margin-top: 270px;
margin-left: 325px;
left: 180px;
left: 180px;
top:-3283px;
top:-3283px;
Line 230: Line 229:
#u564
#u564
{
{
z-index: 32;
width: 91px;
width: 91px;
background-color: transparent;
background-color: transparent;
Line 238: Line 236:
left: 553px;
left: 553px;
top:-3576px;
top:-3576px;
margin-left: 325px;
}
}


#u570
#u570
{
{
z-index: 34;
 
width: 40px;
width: 40px;
background-color: transparent;
background-color: transparent;
Line 250: Line 249:
left: 180px;
left: 180px;
top:-3884px;
top:-3884px;
margin-left: 325px;
}
}


#u582
#u582
{
{
z-index: 36;
 
width: 60px;
width: 60px;
background-color: transparent;
background-color: transparent;
Line 262: Line 262:
left: 315px;
left: 315px;
top:-4436px;
top:-4436px;
margin-left: 325px;
}
}


#u588
#u588
{
{
z-index: 38;
width: 56px;
width: 56px;
background-color: transparent;
background-color: transparent;
Line 274: Line 274:
left: 553px;
left: 553px;
top:-4988px;
top:-4988px;
margin-left: 325px;
}
}


#u600
#u600
{
{
z-index: 40;
width: 30px;
width: 30px;
background-color: transparent;
background-color: transparent;
Line 286: Line 286:
left: 691px;
left: 691px;
top:-5540px;
top:-5540px;
margin-left: 325px;
}
}


#u606
#u606
{
{
z-index: 42;
width: 178px;
width: 178px;
background-color: transparent;
background-color: transparent;
Line 297: Line 297:
margin-top: 402px;
margin-top: 402px;
left: 375px;
left: 375px;
margin-left: 325px;
top:-6092px;
top:-6092px;
}
}
Line 302: Line 303:
#u618
#u618
{
{
z-index: 44;
width: 82px;
width: 82px;
background-color: transparent;
background-color: transparent;
Line 308: Line 308:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 402px;
margin-top: 402px;
margin-left: 325px;
left: 609px;
left: 609px;
top:-6644px;
top:-6644px;
Line 314: Line 315:
#u624
#u624
{
{
z-index: 46;
width: 95px;
width: 95px;
background-color: transparent;
background-color: transparent;
Line 320: Line 320:
margin-right: -10000px;
margin-right: -10000px;
margin-top: 409px;
margin-top: 409px;
margin-left: 325px;
left: 220px;
left: 220px;
top:-7196px;
top:-7196px;
Line 326: Line 327:
#u661
#u661
{
{
z-index: 48;
width: 119px;
width: 119px;
height: 153px;
height: 153px;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
left: 99px;
left: 99px;
top:-7748px;
top:-9245px;
background: transparent url("http://openwetware.org/images/9/94/Board_index_03.jpg") no-repeat left top;
 
}
}


Line 341: Line 342:
height: 153px;
height: 153px;
min-height: 0px;
min-height: 0px;
margin: 0px -10000px 0px 0px;
margin: 0px -10000px 0px 325px;
background: transparent url("http://openwetware.org/images/a/aa/Board_index_rollovers_02_03.jpg") no-repeat left top;
background: transparent url("http://openwetware.org/images/a/aa/Board_index_rollovers_02_03.jpg") no-repeat left top;
}
}
Line 347: Line 348:
#u679
#u679
{
{
z-index: 49;
width: 126px;
width: 126px;
height: 135px;
height: 135px;
position: relative;
position: relative;
margin-left: 325px;
margin-right: -10000px;
margin-right: -10000px;
margin-top: 1px;
margin-top: 1px;
left: 349px;
left: 349px;
top:-7901px;
top:-9109px;
background: transparent url("http://openwetware.org/images/2/24/Board_index_06.jpg") no-repeat left top;
 
}
}


Line 363: Line 364:
height: 135px;
height: 135px;
min-height: 0px;
min-height: 0px;
margin: 1px -10000px 0px 0px;
margin: 1px -10000px 0px 325px;
background: transparent url("http://openwetware.org/images/1/1a/Board_index_rollovers_02_06.jpg") no-repeat left top;
background: transparent url("http://openwetware.org/images/1/1a/Board_index_rollovers_02_06.jpg") no-repeat left top;
}
}
Line 369: Line 370:
#u696
#u696
{
{
z-index: 50;
width: 151px;
width: 151px;
height: 136px;
height: 136px;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 12px;
margin-top: 12px;
left: 570px;
left: 570px;
top:-8037px;
top:-8961px;
background: transparent url("http://openwetware.org/images/b/b9/Board_index_09.jpg") no-repeat left top;
}
}


#u696:hover
#u696:hover
Line 385: Line 386:
height: 136px;
height: 136px;
min-height: 0px;
min-height: 0px;
margin: 12px -10000px 0px 0px;
margin: 12px -10000px 0px 325px;
background: transparent url("http://openwetware.org/images/f/fb/Board_index_rollovers_02_09.jpg") no-repeat left top;
background: transparent url("http://openwetware.org/images/f/fb/Board_index_rollovers_02_09.jpg") no-repeat left top;
}
}
Line 391: Line 392:
#u707
#u707
{
{
z-index: 51;
width: 95px;
width: 95px;
height: 116px;
height: 116px;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 293px;
margin-top: 293px;
left: 220px;
left: 220px;
top:-8185px;
top:-8552px;
background: transparent url("http://openwetware.org/images/4/4d/Board_index_22.jpg") no-repeat left top;
}
}


Line 407: Line 407:
height: 116px;
height: 116px;
min-height: 0px;
min-height: 0px;
margin: 293px -10000px 0px 0px;
margin: 293px -10000px 0px 325px;
background: transparent url("http://openwetware.org/images/f/f1/Board_index_rollovers_02_22.jpg") no-repeat left top;
background: transparent url("http://openwetware.org/images/f/f1/Board_index_rollovers_02_22.jpg") no-repeat left top;
}
}
Line 413: Line 413:
#u718
#u718
{
{
z-index: 52;
 
width: 178px;
width: 178px;
height: 132px;
height: 132px;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 270px;
margin-top: 270px;
left: 375px;
left: 375px;
top:-8594px;
top:-8150px;
background: transparent url("http://openwetware.org/images/e/ef/Board_index_19.jpg") no-repeat left top;
 
}
}


Line 429: Line 430:
height: 132px;
height: 132px;
min-height: 0px;
min-height: 0px;
margin: 270px -10000px 0px 0px;
margin: 270px -10000px 0px 325px;
background: #353334 url("http://openwetware.org/images/1/18/Board_index_rollovers_02_19.jpg") no-repeat left top;
background: #353334 url("http://openwetware.org/images/1/18/Board_index_rollovers_02_19.jpg") no-repeat left top;
}
}
Line 435: Line 436:
#u729
#u729
{
{
z-index: 53;
 
width: 82px;
width: 82px;
height: 94px;
height: 94px;
position: relative;
position: relative;
margin-right: -10000px;
margin-right: -10000px;
margin-left: 325px;
margin-top: 308px;
margin-top: 308px;
left: 609px;
left: 609px;
top:-8996px;
top:-7748px;
background: transparent url("http://openwetware.org/images/2/28/Board_index_25.jpg") no-repeat left top;
}
}


Line 451: Line 452:
height: 94px;
height: 94px;
min-height: 0px;
min-height: 0px;
margin: 308px -10000px 0px 0px;
margin: 308px -10000px 0px 325px;
background: transparent url("http://openwetware.org/images/3/3d/Board_index_rollovers_02_25.jpg") no-repeat left top;
background: transparent url("http://openwetware.org/images/3/3d/Board_index_rollovers_02_25.jpg") no-repeat left top;
}
}


body
#abtitle
{
{
position: relative;
height: 50px;
min-width: 960px;
width: 400px;
padding-top: 36px;
font-family: verdana, Geneva, sans-serif;
padding-bottom: 36px;
font-size: 25px;
position: absolute;
margin-right: -10000px;
top:750px;
left:300px;
text-align: left;
text-decoration:underline;
 
 
}
#abcopy
{ height: 50px;
width: 1000px;
font-family: verdana, Geneva, sans-serif;
font-size: 18px;
position: absolute;
margin-right: -10000px;
top:800px;
left:300px;
text-align: left;
  line-height: 200%;
}
#selfie
{
position: absolute;
margin-right: -10000px;
top:2100px;
left:150px;
}
}


#page .verticalspacer
#body
{
{
clear: both;
position: absolute;
min-width: 960px;
padding-top: 36px;
padding-bottom: 36px;
border: 1px solid transparent;
}
}


Line 472: Line 504:


  <body>
  <body>
 
  <div class="clearfix" id="page"><!-- column -->
   <div id="u450"><!-- image -->
   <div class="clip_frame colelem" id="u450"><!-- image -->
     <img class="block" id="u450_img" src="http://openwetware.org/images/5/58/Board_index_01.jpg" alt="" width="792" height="60"/>
     <img class="block" id="u450_img" src="http://openwetware.org/images/5/58/Board_index_01.jpg" alt="" width="792" height="60"/>
   </div>
   </div>
   <div class="clearfix colelem" id="pu456"><!-- group -->
   <div id="pu456"><!-- group -->
     <div class="clip_frame grpelem" id="u456"><!-- image -->
     <div class="clip_frame grpelem" id="u456"><!-- image -->
     <img class="block" id="u456_img" src="http://openwetware.org/images/c/c1/Board_index_02.jpg" alt="" width="99" height="552"/>
     <img class="block" id="u456_img" src="http://openwetware.org/images/c/c1/Board_index_02.jpg" alt="" width="99" height="552"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u468"><!-- image -->
     <div id="u468"><!-- image -->
     <img class="block" id="u468_img" src="http://openwetware.org/images/d/d4/Board_index_04.jpg" alt="" width="574" height="1"/>
     <img class="block" id="u468_img" src="http://openwetware.org/images/d/d4/Board_index_04.jpg" alt="" width="574" height="1"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u474"><!-- image -->
     <div id="u474"><!-- image -->
     <img class="block" id="u474_img" src="http://openwetware.org/images/a/a0/Board_index_05.jpg" alt="" width="131" height="157"/>
     <img class="block" id="u474_img" src="http://openwetware.org/images/a/a0/Board_index_05.jpg" alt="" width="131" height="157"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u486"><!-- image -->
     <div id="u486"><!-- image -->
     <img class="block" id="u486_img" src="http://openwetware.org/images/c/c3/Board_index_07.jpg" alt="" width="317" height="11"/>
     <img class="block" id="u486_img" src="http://openwetware.org/images/c/c3/Board_index_07.jpg" alt="" width="317" height="11"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u492"><!-- image -->
     <div id="u492"><!-- image -->
     <img class="block" id="u492_img" src="http://openwetware.org/images/6/62/Board_index_08.jpg" alt="" width="95" height="146"/>
     <img class="block" id="u492_img" src="http://openwetware.org/images/6/62/Board_index_08.jpg" alt="" width="95" height="146"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u504"><!-- image -->
     <div id="u504"><!-- image -->
     <img class="block" id="u504_img" src="http://openwetware.org/images/a/a2/Board_index_10.jpg" alt="" width="71" height="540"/>
     <img class="block" id="u504_img" src="http://openwetware.org/images/a/a2/Board_index_10.jpg" alt="" width="71" height="540"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u510"><!-- image -->
     <div id="u510"><!-- image -->
     <img class="block" id="u510_img" src="http://openwetware.org/images/1/1c/Board_index_11.jpg" alt="" width="126" height="22"/>
     <img class="block" id="u510_img" src="http://openwetware.org/images/1/1c/Board_index_11.jpg" alt="" width="126" height="22"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u516"><!-- image -->
     <div id="u516"><!-- image -->
     <img class="block" id="u516_img" src="http://openwetware.org/images/7/73/Board_index_12.jpg" alt="" width="151" height="10"/>
     <img class="block" id="u516_img" src="http://openwetware.org/images/7/73/Board_index_12.jpg" alt="" width="151" height="10"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u522"><!-- image -->
     <div id="u522"><!-- image -->
     <img class="block" id="u522_img" src="http://openwetware.org/images/6/64/Board_index_13.jpg" alt="" width="119" height="5"/>
     <img class="block" id="u522_img" src="http://openwetware.org/images/6/64/Board_index_13.jpg" alt="" width="119" height="5"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u528"><!-- image -->
     <div id="u528"><!-- image -->
     <img class="block" id="u528_img" src="http://openwetware.org/images/6/68/Board_index_14.jpg" alt="" width="81" height="394"/>
     <img class="block" id="u528_img" src="http://openwetware.org/images/6/68/Board_index_14.jpg" alt="" width="81" height="394"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u534"><!-- image -->
     <div id="u534"><!-- image -->
     <img class="block" id="u534_img" src="http://openwetware.org/images/9/99/Board_index_15.jpg" alt="" width="464" height="88"/>
     <img class="block" id="u534_img" src="http://openwetware.org/images/9/99/Board_index_15.jpg" alt="" width="464" height="88"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u540"><!-- image -->
     <div id="u540"><!-- image -->
     <img class="block" id="u540_img" src="http://openwetware.org/images/d/dc/Board_index_16.jpg" alt="" width="77" height="150"/>
     <img class="block" id="u540_img" src="http://openwetware.org/images/d/dc/Board_index_16.jpg" alt="" width="77" height="150"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u546"><!-- image -->
     <div id="u546"><!-- image -->
     <img class="block" id="u546_img" src="http://openwetware.org/images/3/36/Board_index_17.jpg" alt="" width="464" height="24"/>
     <img class="block" id="u546_img" src="http://openwetware.org/images/3/36/Board_index_17.jpg" alt="" width="464" height="24"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u552"><!-- image -->
     <div id="u552"><!-- image -->
     <img class="block" id="u552_img" src="http://openwetware.org/images/7/73/Board_index_18.jpg" alt="" width="195" height="23"/>
     <img class="block" id="u552_img" src="http://openwetware.org/images/7/73/Board_index_18.jpg" alt="" width="195" height="23"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u564"><!-- image -->
     <div id="u564"><!-- image -->
     <img class="block" id="u564_img" src="http://openwetware.org/images/8/85/Board_index_20.jpg" alt="" width="91" height="38"/>
     <img class="block" id="u564_img" src="http://openwetware.org/images/8/85/Board_index_20.jpg" alt="" width="91" height="38"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u570"><!-- image -->
     <div id="u570"><!-- image -->
     <img class="block" id="u570_img" src="http://openwetware.org/images/e/eb/Board_index_21.jpg" alt="" width="40" height="259"/>
     <img class="block" id="u570_img" src="http://openwetware.org/images/e/eb/Board_index_21.jpg" alt="" width="40" height="259"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u582"><!-- image -->
     <div id="u582"><!-- image -->
     <img class="block" id="u582_img" src="http://openwetware.org/images/7/7c/Board_index_23.jpg" alt="" width="60" height="259"/>
     <img class="block" id="u582_img" src="http://openwetware.org/images/7/7c/Board_index_23.jpg" alt="" width="60" height="259"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u588"><!-- image -->
     <div id="u588"><!-- image -->
     <img class="block" id="u588_img" src="http://openwetware.org/images/e/ea/Board_index_24.jpg" alt="" width="56" height="244"/>
     <img class="block" id="u588_img" src="http://openwetware.org/images/e/ea/Board_index_24.jpg" alt="" width="56" height="244"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u600"><!-- image -->
     <div id="u600"><!-- image -->
     <img class="block" id="u600_img" src="http://openwetware.org/images/7/7c/Board_index_26.jpg" alt="" width="30" height="244"/>
     <img class="block" id="u600_img" src="http://openwetware.org/images/7/7c/Board_index_26.jpg" alt="" width="30" height="244"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u606"><!-- image -->
     <div id="u606"><!-- image -->
     <img class="block" id="u606_img" src="http://openwetware.org/images/8/80/Board_index_27.jpg" alt="" width="178" height="150"/>
     <img class="block" id="u606_img" src="http://openwetware.org/images/8/80/Board_index_27.jpg" alt="" width="178" height="150"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u618"><!-- image -->
     <div id="u618"><!-- image -->
     <img class="block" id="u618_img" src="http://openwetware.org/images/4/46/Board_index_28.jpg" alt="" width="82" height="150"/>
     <img class="block" id="u618_img" src="http://openwetware.org/images/4/46/Board_index_28.jpg" alt="" width="82" height="150"/>
     </div>
     </div>
     <div class="clip_frame grpelem" id="u624"><!-- image -->
     <div id="u624"><!-- image -->
     <img class="block" id="u624_img" src="http://openwetware.org/images/9/97/Board_index_29.jpg" alt="" width="95" height="143"/>
     <img class="block" id="u624_img" src="http://openwetware.org/images/9/97/Board_index_29.jpg" alt="" width="95" height="143"/>
     </div>
     </div>
     <div class="grpelem" id="u661"><!-- simple frame --></div>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/video"><div id="u729"><!-- image -->
     <div class="grpelem" id="u679"><!-- simple frame --></div>
    <img class="block" id="u729_img" src="http://openwetware.org/images/2/28/Board_index_25.jpg" alt="" width="82" height="94"/>
     <div class="grpelem" id="u696"><!-- simple frame --></div>
     </div></a>
     <div class="grpelem" id="u707"><!-- simple frame --></div>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/structure"><div id="u718"><!-- image -->
     <div class="grpelem" id="u718"><!-- simple frame --></div>
    <img class="block" id="u718_img" src="http://openwetware.org/images/e/ef/Board_index_19.jpg" alt="" width="178" height="132"/>
    <div class="grpelem" id="u729"><!-- simple frame --></div>
     </div></a>
  </div>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/sponsors2"><div id="u707"><!-- image -->
  <div class="verticalspacer"></div>
    <img class="block" id="u707_img" src="http://openwetware.org/images/4/4d/Board_index_22.jpg" alt="" width="95" height="116"/>
  </div>
     </div></a>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/team"><div id="u696"><!-- image -->
    <img class="block" id="u696_img" src="http://openwetware.org/images/b/b9/Board_index_09.jpg" alt="" width="151" height="136"/>
     </div></a>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/background"><div id="u679"><!-- image -->
    <img class="block" id="u679_img" src="http://openwetware.org/images/2/24/Board_index_06.jpg" alt="" width="126" height="135"/>
     </div></a>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/labbook"><div id="u661"><!-- image -->
    <img class="block" id="u661_img" src="http://openwetware.org/images/9/94/Board_index_03.jpg" alt="" width="119" height="153"/>
    </div></a>  
<div id="abtitle"> <b> Abstract </b></div>
<div id="abcopy"> <br>Protein aggregates are the basis for diseases including Alzheimer’s, Parkinson’s, and Macular Degeneration. <small>[CC1]</small> The ability to detect the protein precursors to these aggregates, often misfolded proteins, provides crucial early-stage diagnostics and monitoring to ultimately improve treatment outcomes. Furthermore, where most biosensing devices simply provide a readout of the presence of the target, we aim to build a device that induces a functional response that aids to clear the target protein or to treat the disease.</br>
 
<br>We <i>hypothesize</i> that aptamer-functionalized DNA origami will bind specific target proteins; undergo conformational changes; and subsequently form oligomeric complexes that elicit a functional response to clear the protein or recruit components to the site of disease. While initial goals are focused on proof of concept with a purely DNA-based system, we will achieve the long term goals through a novel approach of using cryptic binding sites initially buried in the inner part of the device and exposed after the protein-driven conformational change. Our functional response will either expose signaling molecules to drive trafficking or aggregate sensors into defined geometries (inspired by the natural pentamer formation of immunoglobulin M (IgM) that recruits and activates complement, a key component of immune function).</br>
 
<br>The DNA nanostructure is comprised of two plates connected by a DNA hinge referred to hereafter as “BUS” – Biosensing Unit Structure. Gel electrophoresis and transmission electron microscopy (TEM) confirmed well-folded BUS. The BUS units have complementary overhangs to form dimers and trimers, as confirmed by TEM.</br>
 
<br>Future proof of principle experiments will utilize vascular endothelial growth factor (VEGF) and its corresponding aptamer to demonstrate simultaneous protein sequestration and trimer assembly. The BUS may be employed to target amyloids in addition to VEGF to ultimately become a theranostic device for the treatment of Macular Degeneration, Alzheimer’s-, and Parkinson’s disease.</br></div>
<div id="selfie"> <img src="http://openwetware.org/images/1/1e/Teamselfiestick.JPG"> </div>
</div>
</div>
</body>
</body>
</html>
</html>
{{OSUFooter}}
{{OSUFooter}}

Latest revision as of 14:55, 24 October 2015

<html> <style>

  1. content{margin-left:0; margin-top:0; margin-bottom:0;}

.navbar{ top:0px; position:fixed; width:96.8%; } /*clearing the side openwetware panels*/ .firstHeading, #column-one, #p-bookmarks, #p-history , .portlet{ display:none; }

  1. bodyContent/*clearing the side openwetware panels

.firstHeading, #column-one, #p-bookmarks, #p-history , .portlet{ display:none; }

  1. content#bodyContent, {

/*display:none;*/ background-color:#FFFFFF; }

  1. OSUfooter{

clear:both; position:relative; }

  1. footer{display: none;}

<body> <!--css stuff begins here-->

<style>


.html { background-color: #FFFFFF;

}


  1. u450

{

width: 792px; background-color: transparent; position: relative; margin-left: 325px; }

  1. pu456

{ width: 0.01px; }

  1. u456

{ width: 99px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; }

  1. u468

{

width: 574px; position: relative; background-color: transparent; margin-right: -10000px; margin-left: 325px; left: 218px; top:-552px;

}

  1. u474

{

width: 131px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 1px; left: 218px; top:-553px; }

  1. u486

{

width: 317px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 1px; left: 475px; top:-711px; }

  1. u492

{

width: 95px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 12px; left: 475px; top:-723px; }

  1. u504

{

width: 71px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 12px; left: 721px; top:-881px; }

  1. u510

{ width: 126px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 136px; left: 349px; top:-1433px; }

  1. u516

{

width: 151px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 148px; left: 570px; top:-1591px; }

  1. u522

{

width: 119px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 153px; left: 99px; top:-1749px; }

  1. u528

{

width: 81px; background-color: transparent; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 158px; left: 99px; top:-1907px; }

  1. u534

{

width: 464px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 158px; margin-left: 325px; left: 180px; top:-2459px; }

  1. u540

{

width: 77px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 158px; margin-left: 325px; left: 644px; top:-2705px; }

  1. u546

{

width: 464px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 246px; margin-left: 325px; left: 180px; top:-3013px; }

  1. u552

{ width: 195px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 270px; margin-left: 325px; left: 180px; top:-3283px; }

  1. u564

{ width: 91px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 270px; left: 553px; top:-3576px; margin-left: 325px; }

  1. u570

{

width: 40px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 293px; left: 180px; top:-3884px; margin-left: 325px; }

  1. u582

{

width: 60px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 293px; left: 315px; top:-4436px; margin-left: 325px; }

  1. u588

{ width: 56px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 308px; left: 553px; top:-4988px; margin-left: 325px; }

  1. u600

{ width: 30px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 308px; left: 691px; top:-5540px; margin-left: 325px; }

  1. u606

{ width: 178px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 402px; left: 375px; margin-left: 325px; top:-6092px; }

  1. u618

{ width: 82px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 402px; margin-left: 325px; left: 609px; top:-6644px; }

  1. u624

{ width: 95px; background-color: transparent; position: relative; margin-right: -10000px; margin-top: 409px; margin-left: 325px; left: 220px; top:-7196px; }

  1. u661

{ width: 119px; height: 153px; position: relative; margin-right: -10000px; margin-left: 325px; left: 99px; top:-9245px;

}

  1. u661:hover

{ width: 119px; height: 153px; min-height: 0px; margin: 0px -10000px 0px 325px; background: transparent url("http://openwetware.org/images/a/aa/Board_index_rollovers_02_03.jpg") no-repeat left top; }

  1. u679

{ width: 126px; height: 135px; position: relative; margin-left: 325px; margin-right: -10000px; margin-top: 1px; left: 349px; top:-9109px;

}

  1. u679:hover

{ width: 126px; height: 135px; min-height: 0px; margin: 1px -10000px 0px 325px; background: transparent url("http://openwetware.org/images/1/1a/Board_index_rollovers_02_06.jpg") no-repeat left top; }

  1. u696

{ width: 151px; height: 136px; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 12px; left: 570px; top:-8961px; }


  1. u696:hover

{ width: 151px; height: 136px; min-height: 0px; margin: 12px -10000px 0px 325px; background: transparent url("http://openwetware.org/images/f/fb/Board_index_rollovers_02_09.jpg") no-repeat left top; }

  1. u707

{ width: 95px; height: 116px; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 293px; left: 220px; top:-8552px; }

  1. u707:hover

{ width: 95px; height: 116px; min-height: 0px; margin: 293px -10000px 0px 325px; background: transparent url("http://openwetware.org/images/f/f1/Board_index_rollovers_02_22.jpg") no-repeat left top; }

  1. u718

{

width: 178px; height: 132px; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 270px; left: 375px; top:-8150px;

}

  1. u718:hover

{ width: 178px; height: 132px; min-height: 0px; margin: 270px -10000px 0px 325px; background: #353334 url("http://openwetware.org/images/1/18/Board_index_rollovers_02_19.jpg") no-repeat left top; }

  1. u729

{

width: 82px; height: 94px; position: relative; margin-right: -10000px; margin-left: 325px; margin-top: 308px; left: 609px; top:-7748px; }

  1. u729:hover

{ width: 82px; height: 94px; min-height: 0px; margin: 308px -10000px 0px 325px; background: transparent url("http://openwetware.org/images/3/3d/Board_index_rollovers_02_25.jpg") no-repeat left top; }

  1. abtitle

{ height: 50px; width: 400px; font-family: verdana, Geneva, sans-serif; font-size: 25px; position: absolute; margin-right: -10000px; top:750px; left:300px; text-align: left; text-decoration:underline;


}

  1. abcopy

{ height: 50px; width: 1000px; font-family: verdana, Geneva, sans-serif; font-size: 18px; position: absolute; margin-right: -10000px; top:800px; left:300px; text-align: left; 

  line-height: 200%;

}

  1. selfie

{ position: absolute; margin-right: -10000px; top:2100px; left:150px; }

  1. body

{ position: absolute; min-width: 960px; padding-top: 36px; padding-bottom: 36px; border: 1px solid transparent; }

</style> <!--css stuff ends here--> 

<body>

  <div id="u450"><!-- image -->
   <img class="block" id="u450_img" src="http://openwetware.org/images/5/58/Board_index_01.jpg" alt="" width="792" height="60"/>
  </div>
  <div id="pu456"><!-- group -->
   <div class="clip_frame grpelem" id="u456"><!-- image -->
    <img class="block" id="u456_img" src="http://openwetware.org/images/c/c1/Board_index_02.jpg" alt="" width="99" height="552"/>
   </div>
   <div id="u468"><!-- image -->
    <img class="block" id="u468_img" src="http://openwetware.org/images/d/d4/Board_index_04.jpg" alt="" width="574" height="1"/>
   </div>
   <div id="u474"><!-- image -->
    <img class="block" id="u474_img" src="http://openwetware.org/images/a/a0/Board_index_05.jpg" alt="" width="131" height="157"/>
   </div>
   <div id="u486"><!-- image -->
    <img class="block" id="u486_img" src="http://openwetware.org/images/c/c3/Board_index_07.jpg" alt="" width="317" height="11"/>
   </div>
   <div id="u492"><!-- image -->
    <img class="block" id="u492_img" src="http://openwetware.org/images/6/62/Board_index_08.jpg" alt="" width="95" height="146"/>
   </div>
   <div id="u504"><!-- image -->
    <img class="block" id="u504_img" src="http://openwetware.org/images/a/a2/Board_index_10.jpg" alt="" width="71" height="540"/>
   </div>
   <div id="u510"><!-- image -->
    <img class="block" id="u510_img" src="http://openwetware.org/images/1/1c/Board_index_11.jpg" alt="" width="126" height="22"/>
   </div>
   <div id="u516"><!-- image -->
    <img class="block" id="u516_img" src="http://openwetware.org/images/7/73/Board_index_12.jpg" alt="" width="151" height="10"/>
   </div>
   <div id="u522"><!-- image -->
    <img class="block" id="u522_img" src="http://openwetware.org/images/6/64/Board_index_13.jpg" alt="" width="119" height="5"/>
   </div>
   <div id="u528"><!-- image -->
    <img class="block" id="u528_img" src="http://openwetware.org/images/6/68/Board_index_14.jpg" alt="" width="81" height="394"/>
   </div>
   <div id="u534"><!-- image -->
    <img class="block" id="u534_img" src="http://openwetware.org/images/9/99/Board_index_15.jpg" alt="" width="464" height="88"/>
   </div>
   <div id="u540"><!-- image -->
    <img class="block" id="u540_img" src="http://openwetware.org/images/d/dc/Board_index_16.jpg" alt="" width="77" height="150"/>
   </div>
   <div id="u546"><!-- image -->
    <img class="block" id="u546_img" src="http://openwetware.org/images/3/36/Board_index_17.jpg" alt="" width="464" height="24"/>
   </div>
   <div id="u552"><!-- image -->
    <img class="block" id="u552_img" src="http://openwetware.org/images/7/73/Board_index_18.jpg" alt="" width="195" height="23"/>
   </div>
   <div id="u564"><!-- image -->
    <img class="block" id="u564_img" src="http://openwetware.org/images/8/85/Board_index_20.jpg" alt="" width="91" height="38"/>
   </div>
   <div id="u570"><!-- image -->
    <img class="block" id="u570_img" src="http://openwetware.org/images/e/eb/Board_index_21.jpg" alt="" width="40" height="259"/>
   </div>
   <div id="u582"><!-- image -->
    <img class="block" id="u582_img" src="http://openwetware.org/images/7/7c/Board_index_23.jpg" alt="" width="60" height="259"/>
   </div>
   <div id="u588"><!-- image -->
    <img class="block" id="u588_img" src="http://openwetware.org/images/e/ea/Board_index_24.jpg" alt="" width="56" height="244"/>
   </div>
   <div id="u600"><!-- image -->
    <img class="block" id="u600_img" src="http://openwetware.org/images/7/7c/Board_index_26.jpg" alt="" width="30" height="244"/>
   </div>
   <div id="u606"><!-- image -->
    <img class="block" id="u606_img" src="http://openwetware.org/images/8/80/Board_index_27.jpg" alt="" width="178" height="150"/>
   </div>
   <div id="u618"><!-- image -->
    <img class="block" id="u618_img" src="http://openwetware.org/images/4/46/Board_index_28.jpg" alt="" width="82" height="150"/>
   </div>
   <div id="u624"><!-- image -->
    <img class="block" id="u624_img" src="http://openwetware.org/images/9/97/Board_index_29.jpg" alt="" width="95" height="143"/>
   </div>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/video"><div id="u729"><!-- image -->
    <img class="block" id="u729_img" src="http://openwetware.org/images/2/28/Board_index_25.jpg" alt="" width="82" height="94"/>
   </div></a>

<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/structure"><div id="u718"><!-- image --> 

    <img class="block" id="u718_img" src="http://openwetware.org/images/e/ef/Board_index_19.jpg" alt="" width="178" height="132"/>
   </div></a>
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/sponsors2"><div id="u707"><!-- image -->
    <img class="block" id="u707_img" src="http://openwetware.org/images/4/4d/Board_index_22.jpg" alt="" width="95" height="116"/>
   </div></a> 
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/team"><div id="u696"><!-- image -->
    <img class="block" id="u696_img" src="http://openwetware.org/images/b/b9/Board_index_09.jpg" alt="" width="151" height="136"/>
   </div></a> 
<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/background"><div id="u679"><!-- image -->
    <img class="block" id="u679_img" src="http://openwetware.org/images/2/24/Board_index_06.jpg" alt="" width="126" height="135"/>
   </div></a>

<a href="http://openwetware.org/wiki/Biomod/2015/OhioMOD/labbook"><div id="u661"><!-- image --> 

    <img class="block" id="u661_img" src="http://openwetware.org/images/9/94/Board_index_03.jpg" alt="" width="119" height="153"/>
   </div></a>

<div id="abtitle"> <b> Abstract </b></div> <div id="abcopy"> <br>Protein aggregates are the basis for diseases including Alzheimer’s, Parkinson’s, and Macular Degeneration. <small>[CC1]</small> The ability to detect the protein precursors to these aggregates, often misfolded proteins, provides crucial early-stage diagnostics and monitoring to ultimately improve treatment outcomes. Furthermore, where most biosensing devices simply provide a readout of the presence of the target, we aim to build a device that induces a functional response that aids to clear the target protein or to treat the disease.</br>

<br>We <i>hypothesize</i> that aptamer-functionalized DNA origami will bind specific target proteins; undergo conformational changes; and subsequently form oligomeric complexes that elicit a functional response to clear the protein or recruit components to the site of disease. While initial goals are focused on proof of concept with a purely DNA-based system, we will achieve the long term goals through a novel approach of using cryptic binding sites initially buried in the inner part of the device and exposed after the protein-driven conformational change. Our functional response will either expose signaling molecules to drive trafficking or aggregate sensors into defined geometries (inspired by the natural pentamer formation of immunoglobulin M (IgM) that recruits and activates complement, a key component of immune function).</br>

<br>The DNA nanostructure is comprised of two plates connected by a DNA hinge referred to hereafter as “BUS” – Biosensing Unit Structure. Gel electrophoresis and transmission electron microscopy (TEM) confirmed well-folded BUS. The BUS units have complementary overhangs to form dimers and trimers, as confirmed by TEM.</br>

<br>Future proof of principle experiments will utilize vascular endothelial growth factor (VEGF) and its corresponding aptamer to demonstrate simultaneous protein sequestration and trimer assembly. The BUS may be employed to target amyloids in addition to VEGF to ultimately become a theranostic device for the treatment of Macular Degeneration, Alzheimer’s-, and Parkinson’s disease.</br></div> 

<div id="selfie"> <img src="http://openwetware.org/images/1/1e/Teamselfiestick.JPG"> </div>

</div> </div> </body> </html>

<html>

<head> <style>

  1. Footer table {
  width: 96.8%;
  max-width:2350px;
  bottom:0px;
  height:50px;   /* Height of the footer */
  font-weight:300;
  background-color: #2E2E2E;
   text-align:center;
   color: white;
   font-size:13px;
   border: 3px white solid;

}

  1. Footer {
   clear: both; /*may be omitted*/
   position: absolute;
   bottom: 12px; 
   background-color:#fffff;
   width: 100%;
   height: 40px; /* or anything you like */
}


</style> </head> <div id="Footer" > 

       <table>
           <tr>

<td id="osuicon" align="left"><a href="http://www.osu.edu" target="_blank"><img src="http://openwetware.org/images/c/cd/TheOhioStateUniversity-REV-Horiz-RGBHEX.png"height="30" width="190" /></td>

<td id="email">Contact us at <a href="mailto:theohiomod@gmail.com"><font color="white">theohiomod@gmail.com</a></td>

<td id="nblicon"><a href="http://mae.osu.edu/labs/nbl/"target="_blank"><img src="http://openwetware.org/images/e/e9/NBL_logo-RGBHEX.png"height="40" width="40"/></td>

<td id="fbicon"><a href="http://www.facebook.com/OhioMod?fref=photo"target="_blank"><img src="http://openwetware.org/images/3/3f/Facebook_icon.png"height="40" width="40"/></td> 

           </tr>
       </table>
   </div>

</html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2015/OhioMOD&oldid=911641"