Biomod/2015/Kansai/Protocols

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protocol

Experiment

　All staples (cho 1~180, choMD 1~20 and choEND 1~20) and the M13mp18 were mixed together and

annealed from 90˚C to 25˚C at rate of -1.0˚C/min using a PCR thermal cycler.

　At first, we investigated that weather DNA origami structure has cylindrical shape.

We observed it by AFM. Figure 1 is the AFM image. It shows that almost structures have cylindrical shape.

We also performed height analysis. As a result, The height was about 5nm.

Plane DNA origami structure is about 2nm, so we judged that cylindrical shape was constructed.

Fig. 1A AFM image 1

Fig. 1B height analysis