Biomod/2014/idea: Difference between revisions

Latest revision as of 09:22, 25 October 2014

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     <th>
       <a href="http://www.tu-braunschweig.de/index.html"><img src="http://openwetware.org/images/thumb/2/27/Nanoscooter_TUBS-siegel.jpg/800px-Nanoscooter_TUBS-siegel.jpg" width="383" height="142" alt="Logo TU Braunschweig"></a>    
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       width="463" height="142"
       alt="our group"
       title="our group (Nanoscooter) for Biomod competition">
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     <th><img src="http://openwetware.org/images/2/24/Nanoscooter.jpg"
        width="165" height="142" alt="Logo Nanoscooter">  </th>

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<h1> Team Nanoscooter Braunschweig </h1>

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    <th bgcolor="#be1e3c"><center><font size="+1"><a href="Braunschweig"><span style="color:white">Home</span></a></font></center></th>
    <th bgcolor="#be1e3c"><center><font size="+1"><a href="Team"><span style="color:white">Team</span></a></font></center></th>
<th bgcolor="white"><center><font size="+1"><a href="idea"><span style="color:#be1e3c">Project idea</span></a></font></center></th>
    <th bgcolor="#be1e3c"><center><font size="+1"><a href="results"><span style="color:white">Results</span></a></font></center></th>     
    <th bgcolor="#be1e3c"><center><font size="+1"><a href="perspectives"><span style="color:white">Perspectives</span></a></font></center></th>
   
    <th bgcolor="#be1e3c"><center><font size="+1"><a href="Sponsoren"><span style="color:white">Sponsoring</span></a></font></center></th>
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   <td><font size="+2"><u>Project idea: Building the world´s smallest car</u></font>

<p align="justify"> <br> <p align="justify"; style="line-height:2em"><font size="3pt"> DNA is not only a polymer which stores genetic information. Because of its complementary nucleobases DNA provides the opportunity to build complex structures – so called DNA origami – and use them as a template to investigate interactions of molecules, proteins and nanoparticles or as vehicle to transport cargo. Our team designs a nanoscale car consisting of DNA which we called Nanoscooter (from 'Autoscooter', german for 'bumper car'). This Nanoscooter becomes motile on mica surfaces by adapting the kind and concentration of cations in the buffer. Directed movement is achieved by the of repulsion of oxygen gas which is produced at platinum nanoparticles tethered to the back of the Nanoscooter. The shape of the DNA origami is confirmed by atomic force microscopy, while the movement of the car on a flat mica surface is illustrated by fluorescence microscopy using fluorescent beads. This type of DNA origami can be used for directed transport of different components. In the future this technology could be used for the supply in nanoscale factories or as a lithographic pen by controlling the location of nanoscale catalysis. <br><br> To realize our final goal of controlled movement of the Nanoscooter, we assigned several stopovers: <br>

<td>

- <b><a href="Design"><span style="color:">DNA origami design</span></a> and verification:</b><br> Using the <a href="http://cadnano.org/"><span style="color:">caDNAno</span></a> software, a three-dimensional DNA 
  origami structure is designed. <a href="Folding"><span style="color:">Folding</span></a> conditions are adjusted (e.g. magnesium concentration 
  and folding time) and folding is verified using gel electrophoresis and atomic force microscopy 
  (<a href="AFM">AFM</a>).<br><br>
- <b><a href="Functionalization"><span style="color:">Pt-particle functionalization</span></a> and <a href="Attachement"><span style="color:">attachment</span></a> to the Nanoscooter:</b><br> Pt-nanoparticles are 
  functionalized with single stranded DNA. Functionalization is controlled using dynamic light
  scattering (DLS). The Pt-nanoparticles are attached to the Nanoscooter and successful attachment 
  is verified via gel electrophoresis.<br><br>
- <b><a href="Fluorescence"><span style="color:">Fluorescence labeling</span></a>:</b><br> To achieve bright and stable fluorescence labeling, a <a href="Fluorescence"><span style="color:">fluorescent bead</span></a> is 
  attached to the DNA origami using StreptAvidin coated fluorescent beads and in the Nanoscooter 
  incorporated biotin anchors. <br><br>
- <b>Random movement:</b><br> To observe random diffusion on mica surfaces, the suitable cation
  concentrations have to be determined. Movement is visualized on the <a href="AFM"><span style="color:">AFM</span></a> by time-lapse
  imaging and on the fluorescence microscope.<br><br>
- <b>Active movement:</b><br> Directed movement is shown by adding the H<sub>2</sub>O<sub>2</sub> fuel and checking for 
  differences in speed in comparison to the random diffusion.</font></p></i>

</td> </table> </font>

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   <td><img src="http://openwetware.org/images/f/f8/Scooter_II.jpg" width="475" height="350"><br><br><br><br><br><br><br><br><br><br><br><br>
       <img src="http://openwetware.org/images/thumb/6/62/Scooter_I.jpg/800px-Scooter_I.jpg" width="475" height="350"></p>
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Biomod/2014/idea: Difference between revisions

Latest revision as of 09:22, 25 October 2014

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@@ Line 68: / Line 68: @@
 <br>
 <p align="justify"; style="line-height:2em"><font size="3pt">
-DNA is not only a polymer which stores genetic information. Because of its complementary nucleobases DNA provides the opportunity to build complex structures – so called DNA origami – and use them as a template to investigate interactions of molecules, proteins and nanoparticles or as vehicle to transport cargo. Our team designs a nanoscale car consisting of DNA which we called Nanoscooter (from 'Autoscooter', german for 'bumper car'). This Nanoscooter becomes motile on mica surfaces by adapting the kind and concentration of cations in the buffer. Directed movement is achieved by the repulsion of emerging gases that are produced at platinum nanoparticles tethered to the back of the Nanoscooter. The shape of the DNA origami is confirmed by atomic force microscopy, while the movement of the car on a flat mica surface is illustrated by fluorescence microscopy using fluorescent beads. This type of DNA origami can be used for directed transport of different components. In the future this technology could be used for the supply in nanoscale factories or as a lithographic pen by controlling the location of nanoscale catalysis.
+DNA is not only a polymer which stores genetic information. Because of its complementary nucleobases DNA provides the opportunity to build complex structures – so called DNA origami – and use them as a template to investigate interactions of molecules, proteins and nanoparticles or as vehicle to transport cargo. Our team designs a nanoscale car consisting of DNA which we called Nanoscooter (from 'Autoscooter', german for 'bumper car'). This Nanoscooter becomes motile on mica surfaces by adapting the kind and concentration of cations in the buffer. Directed movement is achieved by the of repulsion of oxygen gas which is produced at platinum nanoparticles tethered to the back of the Nanoscooter. The shape of the DNA origami is confirmed by atomic force microscopy, while the movement of the car on a flat mica surface is illustrated by fluorescence microscopy using fluorescent beads. This type of DNA origami can be used for directed transport of different components. In the future this technology could be used for the supply in nanoscale factories or as a lithographic pen by controlling the location of nanoscale catalysis.
 <br><br>
 To realize our final goal of controlled movement of the Nanoscooter, we assigned several stopovers:
@@ Line 85: / Line 85: @@
     origami structure is designed. <a href="Folding"><span style="color:">Folding</span></a> conditions are adjusted (e.g. magnesium concentration
     and folding time) and folding is verified using gel electrophoresis and atomic force microscopy
-    (AFM).<br><br>
+    (<a href="AFM">AFM</a>).<br><br>
   - <b><a href="Functionalization"><span style="color:">Pt-particle functionalization</span></a> and <a href="Attachement"><span style="color:">attachment</span></a> to the Nanoscooter:</b><br> Pt-nanoparticles are
     functionalized with single stranded DNA. Functionalization is controlled using dynamic light
-    scattering (DLS). The Pt-particles are attached to the Nanoscooter and successful attachment
+    scattering (DLS). The Pt-nanoparticles are attached to the Nanoscooter and successful attachment
     is verified via gel electrophoresis.<br><br>
-  - <b>Fluorescent labeling:</b><br> To achieve bright and stable fluorescent labeling, a <a href="Fluorescence"><span style="color:">fluorescent bead</span></a> is
+  - <b><a href="Fluorescence"><span style="color:">Fluorescence labeling</span></a>:</b><br> To achieve bright and stable fluorescence labeling, a <a href="Fluorescence"><span style="color:">fluorescent bead</span></a> is
-    attached to the DNA origami using StreptAvidin coated beads and in the Nanoscooter
+    attached to the DNA origami using StreptAvidin coated fluorescent beads and in the Nanoscooter
     incorporated biotin anchors. <br><br>
   - <b>Random movement:</b><br> To observe random diffusion on mica surfaces, the suitable cation
@@ Line 105: / Line 105: @@
-     <td><img src="http://openwetware.org/images/f/f8/Scooter_II.jpg" width="475" height="350"><br><br>
+     <td><img src="http://openwetware.org/images/f/f8/Scooter_II.jpg" width="475" height="350"><br><br><br><br><br><br><br><br><br><br><br><br>
          <img src="http://openwetware.org/images/thumb/6/62/Scooter_I.jpg/800px-Scooter_I.jpg" width="475" height="350"></p>
      </td>