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<font size="6" color="#000022" face="Arial"><b>Preliminary Experiment</b></font>


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&nbsp;  Preliminary Experiment: verification of FRET with the ssDNAs not modified to the silica<br>
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We firstly confirmed that FRET is surely induced by hybridization of the sword-DNA and the barrel-DNA not yet modified on the silica. We prepared the mixed solutions of FITC/TAMRA-DNA and FITC-DNA/TAMRA-DNA. The concentration is set to 50pM. FITC-DNA/TAMRA-DNA solution was annealed: the solution was kept at 94ºC for 30 seconds and then the temperature was decreased at the rate of 6 ºC degrees per 15 minutes. Fluorescence spectra was measured on Hitachi F-2500 spectrophotometer.<br>




Revision as of 10:07, 25 October 2014

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<a name="header"></a> <img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">



  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Barrel particle</a>
    • <a href="fit_Method.html#b">DNA modified</a>
    • <a href="fit_Method.html#c">樽と人形の結合</a>
      • 　　　
    • <a href="fit_Method.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Method.html#e">Material</a>
  • <a href="fit_Results and Discussion.html#">Result and Discassions</a>
    • <a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a>
    • <a href="fit_Results and Discussion.html#b">DNA modified</a>
    • <a href="fit_Results and Discussion.html#c">樽と人形の結合</a>
    • <a href="fit_Results and Discussion.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Results and Discussion.html#e">Conclusion</a>
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Menber</a>
    • <a href="fit_Member.html#spo">Sponsor</a>


<a name="a"></a>

Preliminary Experiment

  Preliminary Experiment: verification of FRET with the ssDNAs not modified to the silica
We firstly confirmed that FRET is surely induced by hybridization of the sword-DNA and the barrel-DNA not yet modified on the silica. We prepared the mixed solutions of FITC/TAMRA-DNA and FITC-DNA/TAMRA-DNA. The concentration is set to 50pM. FITC-DNA/TAMRA-DNA solution was annealed: the solution was kept at 94ºC for 30 seconds and then the temperature was decreased at the rate of 6 ºC degrees per 15 minutes. Fluorescence spectra was measured on Hitachi F-2500 spectrophotometer.

<a name="b"></a> goal

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<a name="c"></a> Abstract <a name="d"></a>

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Abstract

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<a name="e"></a> Materials

  The DNA samples shown in Table 2 were supplied from sigma-aldrich Japan. Other materials used in this project are summarized in Table 4.


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