Biomod/2014/fit Approach and Goals.html: Difference between revisions

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<table> <tr align="center"> <td cellspacing="10" cellpadding="10" width="1055" width="180" height="60" bgcolor="#BAD3FF"><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka"><B><font face=cursive color="#003366" size="3">Top</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Introduction.html"><B><font face=cursive color="#003366" size="3">Introduction</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Approach and Goals.html"><B><font face=cursive color="#FF6633" size="3" >Approach and Goals</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Method.html"><B><font face=cursive color="#003366" size="3">Method</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1300" width="180" bgcolor="#BAD3FF"><a href="fit_Results and Discussion.html"><B><font face=cursive color="#003366" size="3">Results and Discussion</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Member.html"><B><font face=cursive color="#003366" size="3">Member</font></B></a></td> <td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Sponsor.html"><B><font face=cursive color="#003366" size="3">Sponsor</font></B></a></td> </tr></table>

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<h2 p style="background:#FFFFFF; color:#003366;"><font face=cursive size="5"><B> Approach and Goals </font></B></p></h2>

<h3><p> We paid attention to complementary base pair of DNA Single strand DNA of two can be connected even if I make a mistake, and base pair matches it to some extent. The fewer and fewer mismatched base pair, Cohesion is high. We tried to construct the Pop-up Pirate using DNA and porous silica particle. <br>

<p> <table align="left"> <td><div style="width: 330px;"><img alt="" src="/images/c/c7/Dnakurohige.gif" width="320" height="240" border="0" / ><p> first plan image of figure </p><div></td><tr> <td><div style="width: 330px;"><img alt="" src="/images/b/b7/Fitfiani.gif" width="320" height="240" border="0" / ><p>Structure of fluorescence molecules</p><div></td></table>

 Pop-up Pirate is one of the famous Japanese toy .Players bury a sward into the barrel. When the player bury into the specific position, the doll will be fly away. The barrel is constructed with the single strand DNA binding porous silica particle. Complemental DNA is a roll of the doll. But there are same mismatch between barrel and doll DNAs. Fully complement single strand DNA in a sword. Firstly, doll DNA is mixed with the barrel. Then doll DNA is bound to the barrel with complementally base pairing. Next, sward DNA is added to the solution. The doll DNA would be released firm the barrel because affinity of the sward DNA and the barrel .DNA is strange than that of the doll and the barrel DNAs. At looks like the doll fly away from the barrel. <br>  In order to detect the different state of DNA binding, FRET system is used. The barrel DNA is labeled with fluorescent bye TAMRA, and the barrel DNAs are bound, TAMURA was located near the FITC. <br></p>

  Therefore, FITC is excited by the 488nm laser light and TAMRA is excited by the fluorescent of FITC. <br>

 Then we observed the red fluorescent around the silica particles under the confocal fluorescent microscope. When we added the sward DNA and FITC labeled doll DNA is released from the TAMRA labeled barrel DNA .We could not observed any fluorescent around <br clear="all"><br> </p></h3>

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Biomod/2014/fit Approach and Goals.html: Difference between revisions

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