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<h2>Background</h2>
<h2>Background</h2>
Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi. Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Typi non habent claritatem insitam; est usus legentis in iis qui facit eorum claritatem. Investigationes demonstraverunt lectores legere me lius quod ii legunt saepius. Claritas est etiam processus dynamicus, qui sequitur mutationem consuetudium lectorum. Mirum est notare quam littera gothica, quam nunc putamus parum claram, anteposuerit litterarum formas humanitatis per seacula quarta decima et quinta decima. Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.
The “origami" in <a href="http://www.nature.com/news/2010/100310/full/464158a.html">DNA origami </a> describes the “folding" of long single-stranded DNA (ssDNA) scaffold strands by hybridisation with short oligos. Use of long scaffold strands which span the length of the entire structure appears to improve yields by reducing the possibility of small partial structures forming.  
Traditionally, the scaffold strands used are actually the genomes of viruses! This is because viruses are the most diverse forms of life on Earth, and have found all kinds of crazy ways to store the information in their genome - for instance, in ssDNA. Most DNA origamis have been made using the circular ssDNA genome of bacteriophage M13. However, our <a href="http://openwetware.org/wiki/Biomod/2014/VCCRI/LabBook/Coop">design </a> required a much smaller scaffold strand (912 bases rather than ~ 6400 bases).  
 
<h2>Methods and Materials</h2>
<h2>Methods and Materials</h2>

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<div id="LAB-BOOK-TOP"> <div id="LAB-BOOK-TITLE" style="padding-left:60px; text-align: justify;">Experiment 3 - Characterisation of ssDNA</div> </div> <div id="LAB-BOOK-REPEAT"> <img src="http://openwetware.org/images/8/81/2014-EchiDNA-LAB-BOOK-EXPERIMENT-CLEAN-BOOK.png" /> <a href="#" id="LAB-BOOK-DIRTY-BOOK"></a> <div id="LAB-BOOK-TEXT">

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<h2>Aim</h2> To generate single-stranded DNA of sufficient quantity and quality for assembling our cooperative biosensor.

<h2>Background</h2> The “origami" in <a href="http://www.nature.com/news/2010/100310/full/464158a.html">DNA origami </a> describes the “folding" of long single-stranded DNA (ssDNA) scaffold strands by hybridisation with short oligos. Use of long scaffold strands which span the length of the entire structure appears to improve yields by reducing the possibility of small partial structures forming. Traditionally, the scaffold strands used are actually the genomes of viruses! This is because viruses are the most diverse forms of life on Earth, and have found all kinds of crazy ways to store the information in their genome - for instance, in ssDNA. Most DNA origamis have been made using the circular ssDNA genome of bacteriophage M13. However, our <a href="http://openwetware.org/wiki/Biomod/2014/VCCRI/LabBook/Coop">design </a> required a much smaller scaffold strand (912 bases rather than ~ 6400 bases).


<h2>Methods and Materials</h2> <!-- The following code demonstrates creation of lists. "ul" initiates an unordered list. "li" creates a list item. --> <ul> <li>Item 1</li> <li>Item 2</li> <li>Item 3</li> <li>Item 4</li> <li>Item 5</li> </ul> Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi. Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Typi non habent claritatem insitam; est usus legentis in iis qui facit eorum claritatem. Investigationes demonstraverunt lectores legere me lius quod ii legunt saepius. Claritas est etiam processus dynamicus, qui sequitur mutationem consuetudium lectorum. Mirum est notare quam littera gothica, quam nunc putamus parum claram, anteposuerit litterarum formas humanitatis per seacula quarta decima et quinta decima. Eodem modo typi, qui nunc nobis videntur parum clari, fiant sollemnes in futurum.

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Contact: <a href="mailto:BIOMODaustralia@gmail.com">BIOMODaustralia@gmail.com</a> | ©2014 Team EchiDNA. All rights reserved. This team is supported by the <a id="vccri" href="http://www.victorchang.edu.au">Victor Chang Cardiac Research Institute</a>. | <a id="edit-link" href="">Edit</a>

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