Biomod/2014/UCR/Breaking RNA/Results: Difference between revisions

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<h1>Aptamer-Klemtamer Pairs</h1>
<h1>Aptamer-Klemtamer Pairs</h1>
</font>
</font>
<p> <font size="3.5">
The oligonucleotide sequences are specified in the Supplementary section. Once we designed the necessary genelets and strands for our RNA clocks and switches systems, it was important to characterize and verify that our DNA sequences were designed correctly.
<br><br>
<u>Enzyme Inhibition with RNA Aptamers</u>
<br>
A spectrofluorometer was used to characterize the inhibition of T7 RNA Polymerase and SP6 RNA Polymerase. Gel Electrophoresis was used to validate that RNA aptamer strands were binding to the enzymes.
<br>
<u>DNA Strands Binding to RNA Aptamers</u>
<br>
<br>
<u>Aptamer-Kleptamer Interaction</u>
<br>
</font></p>


<br />
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Revision as of 21:33, 19 October 2014

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Contents



ODE Models


Aptamer-Klemtamer Pairs

The oligonucleotide sequences are specified in the Supplementary section. Once we designed the necessary genelets and strands for our RNA clocks and switches systems, it was important to characterize and verify that our DNA sequences were designed correctly.

Enzyme Inhibition with RNA Aptamers
A spectrofluorometer was used to characterize the inhibition of T7 RNA Polymerase and SP6 RNA Polymerase. Gel Electrophoresis was used to validate that RNA aptamer strands were binding to the enzymes.
DNA Strands Binding to RNA Aptamers

Aptamer-Kleptamer Interaction



Assembling the Circuits


EDIT

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  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA'><span>Home</span></a></li>
  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Project'><span>Motivation & Objectives</span></a></li>
  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Methods'><span>Results</span></a></li>
  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Results'><span>Methods</span></a></li>
  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Members'><span>Supplement</span></a></li>
  <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Achievements'><span>Team</span></a></li>

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