Biomod/2014/OhioMOD/project

From OpenWetWare
Revision as of 12:56, 14 September 2014 by Maxim L. Armstrong (talk | contribs)
Jump to navigationJump to search

<html> <head> <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script> <!-- Latest compiled css and JavaScript --> <script src="http://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script> <!--j--query--> <!--Nav Bar Theme--> <link href="http://maxcdn.bootstrapcdn.com/bootswatch/3.2.0/yeti/bootstrap.min.css" rel="stylesheet"> <style>

  1. content{margin-left:0;}

.navbar{ top:8.5px; position:fixed; width:96.8%; } /*clearing the side openwetware panels*/ .firstHeading, #column-one, #p-bookmarks, #p-history , .portlet{ display:none; }

  1. bodyContent/*clearing the side openwetware panels

.firstHeading, #column-one, #p-bookmarks, #p-history , .portlet{ display:none; }

  1. content#bodyContent, {

/*display:none;*/ background-color:#FFFFFF; }

  1. OSUfooter{

clear:both; }

  1. footer{display: none;}

/*Done clearing*/




</style>

</head>




<!--Nav--> <div class="navbar navbar-default"> 

 <div class="navbar-header">
   <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-responsive-collapse">
     <span class="icon-bar"></span>
     <span class="icon-bar"></span>
     <span class="icon-bar"></span>
   </button>
   <a class="navbar-brand" href="http://openwetware.org/wiki/Biomod/2014/OhioMOD">OhioMOD</a>
 </div>
 <div class="navbar-collapse collapse navbar-responsive-collapse">
   <ul class="nav navbar-nav">
     <li ><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD">Home</a></li>
     <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/project">Background</a></li>

<li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/results">Project and Results</a></li>

<li class="dropdown"> 

       <a href="#" class="dropdown-toggle" data-toggle="dropdown">Materials and Methods <b class="caret"></b></a>
       <ul class="dropdown-menu">
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/protocols">Protocols</a></li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/equipment">Equipment</a></li>
         <li class="divider"></li>
         <li class="dropdown-header">Lab Notebook</li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/LabNotebook">Experiment Schedule</a></li>
         <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/experimentnotes">Experiment Notes</a></li>
       </ul>
     </li>

<li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/team">Team</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/sponsors">Acknowledgement</a></li> <li><a href="http://www.giveto.osu.edu/igive/OnlineGiving/fund_results.aspx?fund=314139" target="_blank">Give To OhioMOD</a></li> 

   </ul>
 </div>

</div> <!--Nav Bar Done-->

</html> <html> <head> <style> center {padding-bottom:50px;}

  1. project{
 width: 700px ;
 margin-left: auto ;
 margin-right: auto ;
 margin-bottom: 50px;

}

/*fixed icon code start*/

  1. fixedbutton {
   position: fixed;
   bottom: 53px;
   right: 10px; }

/*fixed icon code end*/


</style> </head> <div id="project">

<h1>Novel microRNA antisense therapeutic delivery using DNA origami nanostructures (under construction)</h1> Here's the research we did <h2>Introduction</h2> <h2>Background: miRNA & PTEN</h2> MicroRNAs (miRs) are short, non-coding RNAs that regulate gene expression and control essential cellular processes such as cell cycle progression and apoptosis. Dysregulation of miRs expression levels promote tumorigenesis either by elevating oncogene or silencing tumor suppressor gene levels implicating miRs as an attractive therapeutic target. One therapeutic approach is to decrease levels of miRs that target tumor suppressor genes such as PTEN to ultimately promote apoptosis by complementary base pair antagonism via antago-miRs or antisense DNA. However, limitations exist in regard to cellular uptake and the viability of free DNA in the body. A previous study, however, demonstrated that DNA nanostructures functionalized with small interfering RNA (siRNA) underwent cellular entry and executed targeted gene suppression suggesting strong therapeutic potential. Here, we report the fabrication of a rod-like and a block-like DNA nanostructure incorporated with complementary DNA overhangs capable of binding and sequestering miR-21, a PTEN targeting miR that is overexpressed in a variety of malignancies including chronic lymphocytic leukemia (CLL) through the molecular self-assembly process DNA origami.. Proper assembly was confirmed via gel electrophoresis and transmission electron microscopy. Functionalization of miR-21 complementary DNA overhangs was confirmed via fluorescent typhoon gel imaging. In addition, DNA nanostructures were shown to enter the endolysosomal pathway as revealed via fluorescent microscopy. Furthermore, miR-21 complementary DNA overhang functionalized structures induced significant decreases in OSU-CLL cell viability relative to scrambled control overhang nanostructures as shown via fluorescent microscopy and flow cytometry. Moreover, the miR-21 complementary DNA overhang functionalized DNA nanostructures significantly increased PTEN mRNA and protein levels relative to scrambled control overhangs as shown via quantitative real time PCR and immunoblot analysis, respectively. Together, these findings suggest that the complementary miR-21 DNA overhang functionalized DNA nanostructures effectively sequester miR-21 and increase levels of PTEN to induce cytoxicity in OSU-CLL cells. Our results represent a promising antago-miR delivery approach in a CLL leukemia model with implications to a variety of human malignancies.<br> <h2>Background: DNA Origami</h2> <h2>Structures</h2> <h2>miR 21 Sequestering</h2> <h2>Cellular Uptaking</h2> <h2>PTEN Expression</h2> <h2>Cellular Viability</h2> <h2>etc.</h2>

<br><a href="http://openwetware.org/index.php?title=Biomod/2014/OhioMOD/project&action=edit">Edit Project</a><br><br> </div>


<A HREF="#top"><img src="http://openwetware.org/images/4/46/Arrow_up.png" id="fixedbutton"height="40" width="40"></A>


</div> </html>

<html>

<head> <style>

  1. Footer table {
  width: 96.8%;
  max-width:2350px;
  bottom:0px;
  height:50px;   /* Height of the footer */
  font-weight:300;
  background-color: #2E2E2E;
   text-align:center;
   color: white;
   font-size:13px;
   border: 3px white solid;

}

  1. Footer {
   clear: both; /*may be omitted*/
   position: absolute;
   bottom: 12px; 
   background-color:#fffff;
   width: 100%;
   height: 40px; /* or anything you like */
}


</style> </head> <div id="Footer" > 

       <table>
           <tr>

<td id="osuicon" align="left"><a href="http://www.osu.edu" target="_blank"><img src="http://openwetware.org/images/c/cd/TheOhioStateUniversity-REV-Horiz-RGBHEX.png"height="30" width="190" /></td>

<td id="email">Contact us at <a href="mailto:theohiomod@gmail.com"><font color="white">theohiomod@gmail.com</a></td>

<td id="nblicon"><a href="http://mae.osu.edu/labs/nbl/"target="_blank"><img src="http://openwetware.org/images/e/e9/NBL_logo-RGBHEX.png"height="40" width="40"/></td>

<td id="fbicon"><a href="http://www.facebook.com/OhioMod?fref=photo"target="_blank"><img src="http://openwetware.org/images/3/3f/Facebook_icon.png"height="40" width="40"/></td> 

           </tr>
       </table>
   </div>

</html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2014/OhioMOD/project&oldid=820292"