Biomod/2014/OhioMOD/experimentnotes
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<h1>Experiment Notes</h1>
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1. Cellular Uptake Experiment (DNA Extraction)v1 </br> Data 2 </br>Data 3
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<div id="1"> <h3>1.Cellular Uptake Experiment (DNA Extraction) v1</h3> </br><i>07/29/2014</i>
</br><strong>Materials:</strong> </br><li>18 mM Branch folded 7/16 </br><li>OSU CLL cells </br><li>Qiagen DNeasy Blood and Tissue Kit
</br><strong>Procedure:</strong> </br><li>The concentration of Branch was determined using Nanodrop </br><ul id="list2">The concentration was 1.5 nM.</ul> <li>200 uL of OSU CLL cells were washed and incubated in cell media. </br><li>50 uL of structure was added to the cells, and solution was plated and incubated overnight. </br><ul id="list2">Final volume: 250 uL </ul> <ul id="list2">Final concentration of structures: 0.3 nM</ul>
</br><i>07/30/2014</i>
<li>The cells were washed once in PBS, then the total DNA was extracted from the cells according to the Total DNA extraction protocol. <li>A gel was run with the extracted DNA, purified branch structures, 7249 scaffold, and ladder. </br><strong>Results:</strong> </br><img src="http://openwetware.org/images/e/e5/Gel_verification.jpg"height="300" width="300"/>
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