Biomod/2014/OhioMOD/experimentnotes: Difference between revisions

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</br><strong>Procedure:</strong>
</br><strong>Procedure:</strong>
</br><li>The concentration of Branch was determined using Nanodrop
</br><li>The concentration of Branch was determined using Nanodrop
</br><p class="indent">->The concentration was 1.5 nM.</p>
</br><li class="indent" >The concentration was 1.5 nM.</p>
<li>200 uL of OSU CLL cells were washed and incubated in cell media.
<li>200 uL of OSU CLL cells were washed and incubated in cell media.
</br><li>50 uL of structure was added to the cells, and solution was plated and incubated overnight.
</br><li>50 uL of structure was added to the cells, and solution was plated and incubated overnight.
<p class="indent">->Final volume: 250 uL </p>
</br><ul id="list2">Final volume: 250 uL </ul>
<p class="indent">->Final concentration of structures: 0.3 nM</p>
<p class="indent">->Final concentration of structures: 0.3 nM</p>
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Revision as of 14:46, 1 August 2014

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<h1>Experiment Notes</h1>


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1. Cellular Uptake Experiment (DNA Extraction)v1 </br> Data 2 </br>Data 3



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</br><i>07/29/2014</i>

</br><strong>Materials:</strong> </br><li>18 mM Branch folded 7/16 </br><li>OSU CLL cells </br><li>Qiagen DNeasy Blood and Tissue Kit

</br><strong>Procedure:</strong> </br><li>The concentration of Branch was determined using Nanodrop </br><li class="indent" >The concentration was 1.5 nM.</p> <li>200 uL of OSU CLL cells were washed and incubated in cell media. </br><li>50 uL of structure was added to the cells, and solution was plated and incubated overnight. </br><ul id="list2">Final volume: 250 uL </ul> <p class="indent">->Final concentration of structures: 0.3 nM</p> </div>



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