Biomod/2014/Kansai/Sources: Difference between revisions
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'''Protocols'''<br> | |||
・Materials | |||
Staples DNA were purchased from IDT ( U.S.A ) | |||
<br> | |||
Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM. | |||
<div align="center">[[image:1.JPG|450px]]</div> | |||
<br> | |||
<div align="center"><font size="10">↓</font></div> | |||
<br> | |||
These solutions were taken 2 μL each and mixed. | |||
<div align="center">[[image:3.JPG|450px]]</div> | |||
<br> | |||
<div align="center"><font size="10">↓</font></div> | |||
The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands. | |||
<br> | |||
<div align="center">[[image:5.JPG|450px]]</div> | |||
<br> | |||
<div align="center"><font size="10">↓</font></div> | |||
<br> | |||
The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added. | |||
<div align="center">[[image:6.JPG|450px]]</div> | |||
<br> | |||
<div align="center"><font size="10">↓</font></div> | |||
<br> | |||
We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS). | |||
<div align="center">[[image:IMG_1447.JPG|450px]]</div> | |||
<br> | |||
<div align="center"><font size="10">↓</font></div> | |||
<br> | |||
<div align="center">[[image:ex8.png|450px]]</div> | |||
<br> | |||
Revision as of 21:28, 15 September 2014
| Top | Team | Project | Design | Experiment and Result |
protocol | Sources | Sponser |
Protocols
・Materials
Staples DNA were purchased from IDT ( U.S.A )
Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM.
These solutions were taken 2 μL each and mixed.
The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.
The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added.
We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS).
