Biomod/2014/Kansai/Sources: Difference between revisions

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<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai|<font face="Comic Sans MS,cursive,Arial" color="white">Top</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai|<font face="Comic Sans MS,cursive,Arial" color="white">Top</font>]] </td>
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<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Results|<font face="Comic Sans MS,cursive,Arial" color="black">Design</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Results|<font face="Comic Sans MS,cursive,Arial" color="black">Design</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Protocols|<font face="Comic Sans MS,cursive,Arial" color="white">Experiment <br>and Result</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Protocols|<font face="Comic Sans MS,cursive,Arial" color="white">Experiment <br>and Result</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Sources|<font face="Comic Sans MS,cursive,Arial" color="black">Sources</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Sources|<font face="Comic Sans MS,cursive,Arial" color="black">protocol</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Etc.|<font face="Comic Sans MS,cursive,Arial" color="white">Sponsors</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Etc.|<font face="Comic Sans MS,cursive,Arial" color="white">Sources</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/sponser|<font face="Comic Sans MS,cursive,Arial" color="black">Sponser</font>]] </td>
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'''Protocols'''<br>
・Materials
Staples DNA were purchased from IDT ( U.S.A )


<br>
<br>
 
Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM.
<div align="left">
<div align="center">[[image:1.JPG|450px]]</div>
'''・Staples for the components'''<br>
<br>
 
<div align="center"><font size="10">↓</font></div>
</div>
<br>
 
These solutions were taken 2 μL each and mixed.
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<div align="center">[[image:3.JPG|450px]]</div>
{|
<br>
|-
<div align="center"><font size="10">↓</font></div>
|QR 1 || : TTCGCGTCAAAGCCTCAGAGCATAAGAATTAGCAAAATTA
The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.
|-
<br>
|QR 1' || : TTTTTAACACCAAAAACATTATGATTAACATC
<div align="center">[[image:5.JPG|450px]]</div>
|-
<br>
|QR 2 || : TTAACATCCAATAAATCATACAGGAAGCTAAA
<div align="center"><font size="10">↓</font></div>
|-
<br>
|QR 2' || : TCAATTCTACTAATAGTAGTAGCACCCTGTAA
The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added.
|-
<div align="center">[[image:6.JPG|450px]]</div>
|QR 3 || : TTTTTAACACCAAAAA CATTATGATTAACATC
<br>
|-
<div align="center"><font size="10">↓</font></div>
|QR 3DH || : TTTTTAACACCAAAAACATTATGATTAATCCTCTTTTGAGGAACAAGTTTTCTTGGCATC
<br>
|-
We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS).
|QR 4DH || : TCAATTCTCCTCTTTTGAGGAACAAGTTTTCTTGGTACTAATAGTAGTAGCACCCTGTAA
<div align="center">[[image:IMG_1447.JPG|450px]]</div>
|-
<br>
|QR 4' || : CTACCTTTACTAATAGTAGTAGCACCCTGTAA
<div align="center"><font size="10">↓</font></div>
|-
<br>
|QR 5 || : ATTCGCATCGGGAGAAGCCTTTATAGGTGGCA
<div align="center">[[image:ex8.png|450px]]</div>
|-
<br>
|QR 5DH || : ATTC
TCCTCTTTTGAGGAACAAGTTTTCTTGGGCATCGGGAGAAGCCTTTATAGGTGGCA
|-
|QR 6DH || : TTTCATTTGGGGCGCGATCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGAAATTCAACGC
|-
|QR 6' || : ATATAACTGGGGCGCGAGCTGAAATTCAACGC
|-
|QR 6'DH || : ATATAACTGGGGCGCGATCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGAAATTCAACGC
|-
|QR 7 || : ATTTAAATAAATTTTTAGAACCCTAGCTATAT
|-
|QR 7DH || : ATTTAAATAAATTTTTAGAACCCTAGCTTCCTCTTTTGAGGAACAAGTTTTCTTGGATAT
|-
|QR 8DH || : TCGCATCCTCTTTTGAGGAACAAGTTTTCTTGGAATGGTCAATAACCTGTTTCATATATT
|-
|QR 8' || : ATTTAAATAAATTTTTAGAACCCTAGCTATAT
|-
|QR 9 || : GCCCCAAAAATGCCTGAGTAATGTGATACATT
|-
|QR 10DH || : CGAGTAGATTTAGTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCATTAGTAGGTAA
|-
|QR 10' || : TCAAATATTTTAGTTTGACCATTAGTAGGTAA
|-
|QR 10'DH || : TCAAATATTTTAGTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCATTAGTAGGTAA
|-
|QR 11 || : AATCATATAAGGGTGAGAAAGGCCTCTGCGAA
|-
|QR 11DH || : AATCATATAAGGGTGAGAAAGGCCTCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGAA
|-
|QR 12 || : TCCATATAACAGTTGATTCCCAATGGAGACAG
|-
|QR 12' || : CCTAAATTACAGTTGATTCCCAATGGAGACAG
|-
|QR 13 || : TCGATGAACCATCAATATGATATTCCATCAAT
|-
|QR 13DH || : TCGATTCCTCTTTTGAGGAACAAGTTTTCTTGGGAACCATCAATATGATATTAGTTTCAT
|-
|QR 14 || : TGCAACTAAAGTACGGTGTCTGGACAACCGTT
|-
|QR 14DH || : TGATAAATAAGTACGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCTGGACAACCGTT
|-
|QR 14'DH || : TGATAAATAAGTACGGTGTCTGGACAACCGTT
|-
|QR 15 || : ATATAATG CTGTAGCTCAACATGTTTTAAATA
|-
|QR 15DH || : CGGAATCACTGTAGCTCAACATGTTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 15'DH || : CGGAATCACTGTAGCTCAACATGTTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 16 || : AGGTAGAAAGATTCATCAGTTGAGATTGCTGA
|-
|QR 16DH || : AGGTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAAAGATTCATCAGTTGAGATTGCTGA
|-
|QR 17DH || : TTTTTGCGGATGGCTTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCTTAATTTAGGA
|-
|QR 17' || : GTATCATAGATGGCTTAGAGCTTAATTTAGGA
|-
|QR 17'DH || : GTATCATAGATGGCTTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCTTAATTTAGGA
|-
|QR 18 || : AATAAAACTTCAACTAATGCAGATAGAGGTCA
|-
|QR 18DH || : AATAAAACTTCAACTAATGCAGATAGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCA
|-
|QR 19 || : TACCTTTAATTGCTCCTTTTGATAACATAACG
|-
|QR 19' || : ATAAAGCCATTGCTCCTTTTGATAACATAACG
|-
|QR 20 || : CAGTCAGGAATTACGAGGCATAGTTTAGAGAG
|-
|QR 20DH || : CAGTCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGAATTACGAGGCATAGTTTAGAGAG
|-
|QR 21DH || : GAAGCAAACTCCAACAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCAGGAAAGAGCAA
|-
|QR 21' || : GAGAAT
CGCTCCAACAGGTCAGGAAAGAGCAA
|-
|QR 22 || : CTTATGCGTAACCCTCGTTTACCACCAGACCG
|-
|QR 22DH || : CTTATGCGTAACCCTCGTTTACCACCAGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCG
|-
|QR 23DH || : TTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTCGAGCTTCAAAGCGAAGACGACGA
|-
|QR 23' || : AATTTAGGCGAGCTTCAAAGCGAAGACGACGA
|-
|QR 24 || : GTTTAATTAAAATAGCGAGAGGCTATATCGCG
|-
|QR 24DH || : GTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTAAAATAGCGAGAGGCTATATCGCG
|-
|QR 25DH || : AAGAGGAAGCCCGAAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGACTTCAATTTGCAAA
|-
|QR 25' || : AAGAGAATGCCCGAAAGACTTCAATTTGCAAA
|-
|QR 26 || : ACCAGA
ACTGCCAGAGGGGGTAATAAAAGATT
|-
|QR 26DH || : ACCAGAACTGCCAGAGGGGGTAATAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGGATT
|-
|QR 27DH || : TCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 27' || : TAATTCTGAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 28 || : TTCAGTGATGGATAGCGTCCAATATATTATAG
|-
|QR 28DH || : TAATTCTGAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 29DH || : CAAAAATCAGGTCTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGCCCTGACCTGCGGAA
|-
|QR 29' || : TGTTCAGCAGGTCTTTACCCTGACCTGCGGAA
|-
|QR 30 || : CATTACCCAATATTCATTGAATCCCCATAAAT
|-
|QR 31 || : CAGAAAACGAGAATGACCCTCAAA
|-
|QR 31' || : AACAATAGCAGAAAACGAGAATGACCCTCAAA
|-
|QR 32 || : GCTGCGCATCCTGTAGCCAGCTTTAAAAATAA
|-
|QR 33 || : TCGGTTGTCAATAGGAACGCCATCCATCAACA
|-
|QR 34DH || : GGAAACCAGAGCGAGTAACAACCCCAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGTCAT
|-
|QR 35 || : TACTTTTGTAAATTTTTGTTAAATGTCGGATT
|-
|QR 36 || : CCAGCCAGGAACAAACGGCGGATTTTGTTAAA
|-
|QR 36DH || : CCAG
TCCTCTTTTGAGGAACAAGTTTTCTTGGCCAGGAACAAACGGCGGATTTTGTTAAA
|-
|QR 37 || : AAGGATAATGTAAACGTTAATATTGACCGTAA
|-
|QR 38 || : CGTCACCCTCAGCAGCGAAAGACATCAGAAAA
|-
|QR 38DH || : CGTCATCCTCTTTTGAGGAACAAGTTTTCTTGGCCCTCAGCAGCGAAAGACATCAGAAAA
|-
|QR 39 || : AGATTCAAGTACCCCGGTTGATAAGCATCGGA
|-
|QR 40DH || : TTGCAGGGAGCAACGGCTACAGAGAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGTGTC
|-
|QR 41 || : TCAAATCACGGTAATCGTAAAACTGCTTTGAG
|-
|QR 42 || : CACGCATAACTTTTTCATGAGGAACAAGAGAA
|-
|QR 42DH || : CTAGCTGAGAGAGT
CTGGAGCAAAGTTTCCAT
|-
|QR 43 || : CTAGCTGAGAGAGT
CTGGAGCAAAGTTTCCAT
|-
|QR 43DH || : CTAGCTGAGAGAGTCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCAAAGTTTCCAT
|-
|QR 44 || : CATTGCCTTAAATTAATGCCGGAGAGGGTAGC
|-
|QR 45DH || : TATTTTTGAGAGATCTACAAAGGCTATCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGT
|-
|QR 46 || : CGAGGTGACCTAAAACGAAAGAGGATTATTAC
|-
|QR 46DH || : CGAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTGACCTAAAACGAAAGAGGATTA
TTAC
|-
|QR 47 || : ATACCACAGAACTAACGGAACAACCAAAAGAA
|-
|QR 47DH || : ATACCACAGAACTAACGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAACAACCAAAAGAA
|-
|QR 48 || : CCTTTAATAACACTCATCTTTGACTCTACGTT
|-
|QR 48DH || : CCTTTAATAACACTCATCTTTGACTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGCGTT
|-
|QR 49 || : CCAAAAGGACGTTGGGAAGAAAAACCCCAGCG
|-
|QR 50 || : GAAAATCTAAGCGCGAAACAAAGTCATTATAC
|-
|QR 50DH || : GAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCTAAGCGCGAAACAAAGTCATTATAC
|-
|QR 51 || : CACTATCAATTTTAAGAACTGGCTACAACGGA
|-
|QR 51DH || : CACTATCAATTTTAAGATCCTCTTTTGAGGAACAAGTTTTCTTGGACTGGCTACAACGGA
|-
|QR 52 || : AAAGGAATTCATCGCCTGATAAATTGAATTAC
|-
|QR 52DH || : AAAGGAATTCATCGCCTGATAAAT
TGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTAC
|-
|QR 53 || : TAAAAACCTCAACTTTAATCATTGTGTGTCGA
|-
|QR 54 || : TTTCAGCGACCTGCTCCATGTTACTTGAGATG
|-
|QR 54DH || : TTTCATCCTCTTTTGAGGAACAAGTTTTCTTGGGCGACCTGCTCCATGTTACTTGAGATG
|-
|QR 55 || : AGAAGTTTGAGTAGTAAATTGGGCTTAGCCGG
|-
|QR 56 || : TGTATGGGGCAGACGGTCAATCATCGAGAAAC
|-
|QR 56DH || : TGTATG
GGGCAGACGGTCAATCATCGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAC
|-
|QR 57 || : GTTTAGACATAAGGCTTGCCCTGAAAGGGAAC
|-
|QR 58 || : TCGTCTTTCCAACTTTGAAAGAGGGCTGCTCA
|-
|QR 58DH || : TCGTCTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTCCAACTTTGAAAGAGGGCTGCTCA
|-
|QR 59 || : TCGTCATAAAATCAACGTAACAAAACAGATGA
|-
|QR 59DH || : TCGTCATAAAATCAACGTCCTCTTTTGAGGAACAAGTTTTCTTGGTAACAAAACAGATGA
|-
|QR 60 || : CCTCATAGCAGACCAGGCGCATAGCGGATATT
|-
|QR 61 || : TGCTTTAATAATCTTGACAAGAACGCTGGCTG
|-
|QR 62 || : ACGGCCAGTGGGAAGGGCGATCGGCCATTCAG
|-
|QR 63 || : TTAAATGTGGCAAAGCGCCATTCGTGCGGGCC
|-
|QR 64 || : CCTCATTAAAGCCAGAATGGAAAGATCGCACT
|-
|QR 64DH || : CCTCTCCTCTTTTGAGGAACAAGTTTTCTTGGATTAAAGCCAGAATGGAAAGATCGCACT
|-
|QR 65DH || : TGGGATAGTATCGGCCTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGGAAGCGCAGTCT
|-
|QR 66 || : GACGACAGGTCACGTTGGTGTAGATGGGCGCA
|-
|QR 67 || : TCGTAACCGTGCATCTGC
CAGTTTGAGGGGAC
|-
|QR 67DH || : TCGTAACCGTGCATCTGCCAGTTTGAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGGAC
|-
|QR 68 || : CCAGCATTATGATACAGGAGTGTATGCGGGAT
|-
|QR 68DH || : CCAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGATTATGATACAGGAGTGTATGCGGGAT
|-
|QR 69 || : ACGAGGGTAGTTAAAGGCCGCTTTCTGGTAAT
|-
|QR 70 || : AGCCGCCAACGGGGTCAGTGCCTTGCTGAGGC
|-
|QR 70DH || : AGCCGCCAACGGGGTCAGTGCCTTGCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGC
|-
|QR 71 || : GACTAAAGACCGATATATTCGGTCGAGTAACA
|-
|QR 72 || : AGAACCGCATAAACAGTTAATGCCCCATCGCC
|-
|QR 72DH || : AGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGCGCATAAACAGTTAATGCCCCATCGCC
|-
|QR 73 || : TAAACGGGCCGACAATGACAACAACCCTGCCT
|-
|QR 73DH || : TAAACGGGCCGACAATGTCCTCTTTTGAGGAACAAGTTTTCTTGGACAACAACCCTGCCT
|-
|QR 74 || : TAGTTGCGTAAAATACGTAATGCCACTACGAA
|-
|QR 75 || : CCGGAACCACCTATTATTCTGAAAGATACCGA
|-
|QR 75DH || : CCGGAACCACCTATTATTCTGAAAGATATCCTCTTTTGAGGAACAAGTTTTCTTGGCCGA
|-
|QR 76 || : GGCACCAAATTTCTTAAACAGCTTCATGAAAG
|-
|QR 77 || : CATAATCAGGCTGAGACTCCTCAACTTGCTTT
|-
|QR 77DH || : CATAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCAGGCTGAGACTCCTCAACTTG
CTTT
|-
|QR 78 || : TACACTAATGTATCGGTTTATCAGGAGAAGGA
|-
|QR 78DH || : TACACTAATGTATCGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCAGGAGAAGGA
|-
|QR 79 || : CATAGCCCAGCGGGGTTTTGCTCAAAAAGGAG
|-
|QR 79DH || : CATAGCCCAGCGGGGTTTTGCTCAAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGGGAG
|-
|QR 80 || : ATTATACCCCAAAAAAAAGGCTCCGTACCAGG
|-
|QR 81 || : GACTGTAGTGCCGTCGAGAGGGTTTTCACGTT
|-
|QR 82DH || : GATTTGTATGCGAATAATCCTCTTTTGAGGAACAAGTTTTCTTGGTAATTTTGATATAAG
|-
|QR 83 || : TAGCGACAGGAATAGGTGTATCACGAACAACT
|-
|QR 83DH || : TAGCGACAGGAATAGGTGTATCACGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGAACT
|-
|QR 84 || : AATCCGCGGAGTGAGAATAGAAAGCGTACTCA
|-
|QR 85 || : AATGAAACAGTACCGCCACCCTCATTCAACAG
|-
|QR 85DH || : AATGATCCTCTTTTGAGGAACAAGTTTTCTTGGAACAGTACCGCCACCCTCATTCAACAG
|-
|QR 86 || : AACGAGGCATTTTGCTAAACAACTGAACCGCC
|-
|QR 86DH || : AACGAGGCATTTTGCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAACAACTGAACCGCC
|-
|QR 87DH || : CCATTACCAACCGCCACCCTCAGAGAATTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTC
|-
|QR 88 || : CGAACTGACCAGACGTTAGTAAATGCCACCAC
|-
|QR 89 || : GGGAATTATCAGGGATAGCAAGCCAAGTTTTG
|-
|QR 89DH || : GGGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCAGGGATAGCAAGCCAAGTTTTG
|-
|QR 90 || : ACGGTGTATTAGCGTAACGATCTACAATAGGA
|-
|QR 90DH || : ACGGTGTATTAGCGTAATCCTCTTTTGAGGAACAAGTTTTCTTGGCGATCTACAATAGGA
|-
|QR 91 || : TGAATTATACCGTAACACTGAGTTCAGACAGC
|-
|QR 92 || : ACCTTCATCGCCTGTAGCATTCCATCGTCACC
|-
|QR 93 || : GACAGGAAGCTTGCATGCCTGCAGGTAAAACG
|-
|QR 94 || : TCTTCGCTTCCCAGTCACGACGTTGTCGACTC
|-
|QR 95 || : CAGGGTTTATTACGCCAGCTGGCGAAAGGGGG
|-
|QR 96 || : AGCTAAACCCCCGGGTACCGAGCTCGAATTCG
|-
|QR 97 || : ATGTGCTGCAAGGCGATTAAGTTGGGTAACGC
|-
|QR 97DH || : ATGTGCTGCAAGGCGATTAAGTTGGGTATCCTCTTTTGAGGAACAAGTTTTCTTGGACGC
|-
|QR 98 || : TAACGTGCGTCATAGCTGTTTCCTAAATAAAT
|-
|QR 98DH || : TAACTCCTCTTTTGAGGAACAAGTTTTCTTGGGTGCGTCATAGCTGTTTCCTAAATAAAT
|-
|QR 99DH || : CTGAATTTCTTGATATTTCCTCTTTTGAGGAACAAGTTTTCTTGGCACAAACGTGTGAAA
|-
|QR 100 || : CGATTGGCACCGTTCCAGTAAGCGTCATACAT
|-
|QR 101DH || : GCGCGTACCGCTCACAATTCCACAAGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGA
|-
|QR 102 || : GGCTTTTGGACAGGAGGTTGAGGCCAACATAC
|-
|QR 103DH    || : CACACTCCTCTTTTGAGGAACAAGTTTTCTTGGCCGAGCATAAAGTGTAAAGAGCCGCCG
|-
|QR 104 || : AAGTTTTACCAGAACCACCACCAGCCTGGGGT
|-
|QR 105DH || : GCAAGTGTAGTGAGCTAACTCACAACCCTCCTCTTTTGAGGAACAAGTTTTCTTGGTCAG
|-
|QR 106 || : GTGCCCGTCACCCTCAGAGCCACCTTAATTGC
|-
|QR 107 || : AGAAAGCGCACTGCCCGCTTTCCACCACCCTC
|-
|QR 108 || : ATTTCGGAGCCTCCCTCAGAGCCGGTCGGGAA
|-
|QR 108DH || : ATTTCGGAGCCTCCCTCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGAGCCGGTCGGGAA
|-
|QR 109 || : AAAGCCGGTGCCAGCTGCATTAATAGCCACCA
|-
|QR 109DH || : AAAGCCGGTGCCAGCTGCATTAATAGCCTCCTCTTTTGAGGAACAAGTTTTCTTGGACCA
|-
|QR 110 || : TATTAAGAAAATCACCGGAACCAGGAATCGGC
|-
|QR 111 || : AAAGGGAGGGGGAGAGGCGGTTTGCATCTTTT
|-
|QR 111DH || : AAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAGGGGGAGAGGCGGTTTGCATCTTTT
|-
|QR 112 || : TTAGGATTCCTTATTAGCGTTTGCCGTATTGG
|-
|QR 112DH || : TTAGGATTCCTTATTAGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGTTTGCCGTATTGG
|-
|QR 113 || : CGAGGTGCGTGGTTTTTCTTTTCATTTTCGGT
|-
|QR 113DH || : CGAGGTGCGTGGTTTTTCTTTTCATTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGT
|-
|QR 114 || : CGGATAAGCGCGTTTTCATCGGCACCAGTGAG
|-
|QR 115 || : TGAACCATCAGCTGATTGCCCTTCTAGCGTCA
|-
|QR 115DH || : TGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGCATCAGCTGATTGCCCTTCTAGCGTCA
|-
|QR 116 || : TATAGCCCGAATCAAGTTTGCCTTACCGCCTG
|-
|QR 116DH || : TATAGCCCGAATCAAGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTGCCTTACCGCCTG
|-
|QR 117 || : AAAACCGTAGAGTTGCAGCAAGCGGTAATCAG
|-
|QR 117DH || : AAAACCGTAGAGTTGCAGCAAGCGGTAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCAG
|-
|QR 118 || : GGAGGTTTCATCGATAGCAGCACCGTCCACGC
|-
|QR 119DH || : TAAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAACCCCAGCAGGCGAAAATACGTCACC
|-
|QR 120DH || : ACCCTCAGATTAGCAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGCCGGAACCTGTTTG
|-
|QR 121DH || : TGTTGTTCTCCGAAATCGGCAAAACAGTTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCA
|-
|QR 122 || : CCTCATTTGAGCCAGCAAAATCACTCCCTTAT
|-
|QR 123DH || : ACCCATGTCACCGTCACTCCTCTTTTGAGGAACAAGTTTTCTTGGCGACTTGAGCCATTT
|-
|QR 124 || : CACCACGGGGCGACATTCAACCGAATTAAAGG
|-
|QR 125 || : AGTACAAATTGACGGAAATTATTCTTGAGGGA
|-
|QR 126 || : AAAAGTTTCGCCAGAATCCTGAGAGGATTTTA
|-
|QR 127 || : TAGAGGATAGGAGGCCGATTAAAGAGTGTTTT
|-
|QR 128 || : ATTAAATCGTGAGGCCACCGAGTAAGAGCGGG
|-
|QR 128DH || : ATTAAATCGTGAGGCCACCGAGTAAGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGG
|-
|QR 129 || : TAATCATGTTTCCTCGTTAGAATCAAAGAGTC
|-
|QR 130 || : TATTAGACACGCAAATTAACCGTTAGCACGTA
|-
|QR 130DH || : TATTTCCTCTTTTGAGGAACAAGTTTTCTTGGAGACACGCAAATTAACCGTTAGCACGTA
|-
|QR 131 || : TTGTTATCTATGGTTGCTTTGACGGTAGCAAT
|-
|QR 131DH || : TTGTTATCTATGGTTGCTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTGACGGTAGCAAT
|-
|QR 132DH || : GTCAATAGGATTAGTAATAACATCGCTATCCTCTTTTGAGGAACAAGTTTTCTTGGCAGG
|-
|QR 133 || : GAGCCGGACCGCGCTTAATGCGCCACTTGCCT
|-
|QR 134 || : TTAGGAGCGAACTCAAACTATCGGGTAACCAC
|-
|QR 134DH || : TTAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCGAACTCAAACTATCGGGTAACCAC
|-
|QR 135 || : GCCTAATGAGCGGTCACGCTGCGCCCTTGCTG
|-
|QR 135DH || : GCCTAATGAGCGGTCACTCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGCGCCCTTGCTG
|-
|QR 136 || : AAAGGAATCAGAACAATATTACCGGGGCGCTG
|-
|QR 136DH || : AAAGGAATCAGAACAATATTACCGGGGCTCCTCTTTTGAGGAACAAGTTTTCTTGGGCTG
|-
|QR 137 || : GTTGCGCTAAAGGAGCGGGCGCTACCAGCCAT
|-
|QR 138 || : CAATATCTGAAAAACGCTCATGGAGGAAGGGA
|-
|QR 139 || : ACCTGTCGCGAACGTGGCGAGAAAAATACCTA
|-
|QR 139DH || : ACCTGTCGCGAACGTGGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGAGAAAAATACCTA
|-
|QR 140 || : CCTTGCTGCGCTCAATCGTCTGAATGACGGGG
|-
|QR 140DH || : CCTTGCTGCGCTCAATCGTCTGAATGACTCCTCTTTTGAGGAACAAGTTTTCTTGGGGGG
|-
|QR 141 || : CAACGCGCCCCCCGATTTAGAGCTATGGATTA
|-
|QR 142 || : AGCCAGCAGGCAGATTCACCAGTCGGAACCCT
|-
|QR 142DH || : AGCCATCCTCTTTTGAGGAACAAGTTTTCTTGGGCAGGCAGATTCACCAGTCGGAACCCT
|-
|QR 143 || : GCGCCAGGCGTAAAGCACTAAATCACACGACC
|-
|QR 143DH || : GCGCCAGGCGTAAAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGCTAAATCACACGACC
|-
|QR 144 || : ATTAACACAAGGGACATTCTGGCCTTTGGGGT
|-
|QR 144DH || : ATTAACACAAGGGACATTCTGGCCTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGGGGT
|-
|QR 145 || : ACGGGCAACACCCAAATCAAGTTTAACAGAGA
|-
|QR 146 || : GCAGAAGATTCTGACCTGAAAGCGCCACTACG
|-
|QR 146DH || : GCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGATTCTGACCTGAAAGCGCCACTACG
|-
|QR 147 || : GCCCTGAGCTATCAGGGCGATGGCTAAGAATA
|-
|QR 147DH || : GCCCTGAGCTATCAGGGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGATGGCTAAGAATA
|-
|QR 148 || : CATCGCCAAGACAATATTTTTGAAAAGGGCGA
|-
|QR 148DH || : CATCGCCAAGACAATATTTTTGAAAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGCGA
|-
|QR 149 || : TGGTTTGCGTGGACTCCAACGTCATGGCTATT
|-
|QR 150DH || : ATGGTGGTCAGTTTGGATCCTCTTTTGAGGAACAAGTTTTCTTGGACAAGAGTCCACTAT
|-
|QR 151 || : ATAGCTATAGGAAACGCAATAATAGGGTTGAG
|-
|QR 151DH || : ATAGCTATAGGAAACGCAATAATAGGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTGAG
|-
|QR 152 || : AAATCAAAAGAATAGCCCGAGATAACGGAATA
|-
|QR 153 || : ATAGAAAATTCATATGGTTTACCAGCGCCAAA
|-
|QR 153DH || : ATAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAAATTCATATGGTTTACCAGCGCCAAA
|-
|QR 154 || : GACAAAAGAATAAGTTTATTTTGTCACAATCA
|-
|QR 154DH || : GACAAAAGAATAAGTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGATTTTGTCACAATCA
|-
|QR 155 || : AGAGAGATTATGTTAGCAAACGTACGCAAAGA
|-
|QR 156 || : GGGAAGGTCAACATATAAAAGAAAGAAAATAC
|-
|QR 157 || : TAGCGATACATTATCATTTTGCGGTTAATTTT
|-
|QR 158 || : TATAATCACTTTGCCCGAACGTTAAACAAAGA
|-
|QR 159 || : TAATTAATGAAGGAGCGGAATTATCAACTCGT
|-
|QR 159DH || : TAATTAATGAAGGAGCGGAATTATCAACTCCTCTTTTGAGGAACAAGTTTTCTTGGTCGT
|-
|QR 160 || : TGTCCATCTTTACAAACAATTCGACATCATAT
|-
|QR 161 || : TGAATAACATCAGATGATGGCAATTTTAGAAG
|-
|QR 161DH || : TGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTAACATCAGATGATGGCAATTTTAGAAG
|-
|QR 162 || : ACTTCTTTATAATACATTTGAGGATCATCAAT
|-
|QR 163 || : ATGGAAACGATTGTTTGGATTATATTAGAGCC
|-
|QR 163DH || : ATGGAAACGATTGTTTGGATTATATTAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCC
|-
|QR 164 || : GAGTAGAAACTAACAACTAATAGACTTCTGAA
|-
|QR 165 || : ATTTAACAGGGTTAGAACCTACCAAAATATCT
|-
|QR 165DH || : ATTTATCCTCTTTTGAGGAACAAGTTTTCTTGGACAGGGTTAGAACCTACCAAAATATCT
|-
|QR 166 || : GTAATATCTGAGGAAGGTTATCTATATCAAAA
|-
|QR 166DH || : GTAATATCTGAGGAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCTATATCAAAA
|-
|QR 167DH || : GAAACAAAACGTAAAACAGAAATAAACATCCTCTTTTGAGGAACAAGTTTTCTTGGGTTG
|-
|QR 168 || : TGCAACAGGGTCAGTTGGCAAATCAAGAAATT
|-
|QR 169 || : TCATTTCATTTCAGGTTTAACGTCCCCTCAAT
|-
|QR 170DH || : CATTTTGAAACCTCAAATCCTCTTTTGAGGAACAAGTTTTCTTGGTATCAAAAGATGAAT
|-
|QR 171 || : CCAAGTTAACAGTACCTTTTACATAAGCATCA
|-
|QR 172 || : TTTACATTGCAAATGAAAAATCTACGGGAGAA
|-
|QR 173 || : AGTAATAACGCCTGCAACAGTGCC ACGCTGAG
|-
|QR 173DH || : AGTAATAACGCCTGCAATCCTCTTTTGAGGAACAAGTTTTCTTGGCAGTGCCACGCTGAG
|-
|QR 174 || : CGCGAGGCTGCACCCAGCTACAATCGGTCAGT
|-
|QR 174DH || : CGCGAGGCTGCACCCAGCTACAATCGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGT
|-
|QR 175 || : TAGAACCCTAAAACAGAGGTGAGGTTTATCCT
|-
|QR 176 || : AGATATAGCCAACGCTAACGAGCGAACCACCA
|-
|QR 177 || : CGTGGCACTTAAAAATACCGAACGTCTTTCCA
|-
|QR 177DH || : CGTGGCACTTAAAAATATCCTCTTTTGAGGAACAAGTTTTCTTGGCCGAACGTCTTTCCA
|-
|QR 178 || : GAATCATTTTTGCCAGTTACAAAACCCTAAAA
|-
|QR 179 || : AGTCTTTAATGCGCGAACTGATAGTAAACAGC
|-
|QR 180 || : AGTAAGCAGATAGCCGAACAAAGTTACCAGAA
|-
|QR 181 || : GGAAACCGCTTACCGAAGCCCTTTTTAAGAAA
|-
|QR 182 || : ACGGGTATTTTAACGTCAAAAATGAAATAGCA
|-
|QR 182DH || : ACGGGTATTTTAACGTCAAAAATGAAATTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCA
|-
|QR 183 || : CCCAAAAGAGAGCAAGAAACAATGAAAATAGC
|-
|QR 184 || : CAATAATAAACTGGCATGATTAAGACTCCTTA
|-
|QR 185 || : ATAATCGGCAGAGAGAATAACATAGTTAAGCC
|-
|QR 185DH || : ATAATTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGCAGAGAGAATAACATAGTTAAGCC
|-
|QR 186 || : TTACGCAGAACCCACAAGAATTGAAAAACAGG
|-
|QR 186DH || : TTACGCAGAACCCACAATCCTCTTTTGAGGAACAAGTTTTCTTGGGAATTGAAAAACAGG
|-
|QR 187 || : ATCCTAATTTAGACGGGAGAATTACTAATATC
|-
|QR 188 || : ATACATAAAGAGGGTAATTGAGCGACTGAACA
|-
|QR 189 || : CAAAATTAATTAAGACGCTGAGAATGAAAACA
|-
|QR 189' || : ATTAAGACGCTGAGAATGAAAACA
|-
|QR 190 || : AACCACCATTTCCCTTAGAATCCTGAGTCAAT
|-
|QR 191 || : CAATAAATTATCAAAATCATAGGTGTCGCTAT
|-
|QR 191DH || : CAATAAATTATCAAAATCATAGGTGTCGTCCTCTTTTGAGGAACAAGTTTTCTTGGCTAT
|-
|QR 191'DH || : AGTGAATTTATCAAAATCATAGGTGTCGTCCTCTTTTGAGGAACAAGTTTTCTTGGCTAT
|-
|QR 192 || : TCCTGATTCTTGCTTCTGTAAATCCTGAGAGA
|-
|QR 193 || : TCAATTCTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 193DH || : TCAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTCTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 193' || : CTACCTTTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 194 || : ATAATCCTAGTACATAAATCAATAGTTGGGTT
|-
|QR 194DH || : ATAATCCTAGTACATAATCCTCTTTTGAGGAACAAGTTTTCTTGG ATCAATAGTTGGGTT
|-
|QR 195 || : TTTCATTTATAT
GTAAATGCTGATCTTTTTTA
|-
|QR 195DH || : TTTCATTTATAT
GTAAATGCTGATCTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTA
|-
|QR 195'DH || : ATATAACTATAT
GTAAATGCTGATCTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTA
|-
|QR 196 || : TAATGGAAATTTCATTTGAATTACGCAAATCC
|-
|QR 197 || : TCGCAAATGACAAAGAACGCGAGATTAATTAC
|-
|QR 197DH || : TCGCATCCTCTTTTGAGGAACAAGTTTTCTTGGAATGACAAAGAACGCGAGATTAATTAC
|-
|QR 197' || :AATCGCAAGACAAAGAACGCGAGATTAATTAC
|-
|QR 198 || : TTATTTGCCATCAAGAAAACAAAAAAACTT
TT
|-
|QR 198DH || : TTATTTGCCATCAAGAATCCTCTTTTGAGGAACAAGTTTTCTTGG AACAAAAAAACTT
TT
|-
|QR 199 || : CGAGTAGAATTTTAGTTAATTTCA AAGATGAT
|-
|QR 199DH || : CGAGTAGAATTTTAGTTAATTTCAAAGATCCTCTTTTGAGGAACAAGTTTTCTTGG TGAT
|-
|QR 199'DH || : TCAAATATATTTTAGTTAATTTCAAAGATCCTCTTTTGAGGAACAAGTTTTCTTGGTGAT
|-
|QR 200 || : GCGTAGATATTACCTGAGCAAAAGTCTTCTGA
|-
|QR 201 || : TCCATATATAATGGTTTGAAATACCGAATTAT
|-
|QR 201' || : CCTAAATTTAATGGTTTGAAATACCGAATTAT
|-
|QR 202DH || : ATACAGTACAAAATCGCTCCTCTTTTGAGGAACAAGTTTTCTTGG GCAGAGGCGACCGTG
|-
|QR 203 || : TGCAACTAAAGGCGTTAAATAAGATTTGAATA
|-
|QR 203DH || : TGCAACTAAAGGCGTTAAATAAGATTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 203'DH || : TGATAAATAAGGCGTTAAATAAGATTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 204 || : ACAATAACGGATTCGCCTGATTGCATAAACAC
|-
|QR 205 || : GGGAGGTTTTGAAGCCTTAAATCA AGATTAGT
|-
|QR 205DH || : GGGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTTTTGAAGCCTTAAATCA AGATTAGT
|-
|QR 206 || : TGCTATTTGTTTTAGCGAACCTCC CGACTTGC
|-
|QR 206DH || : TGCTATTTGTTTTAGCGTCCTCTTTTGAGGAACAAGTTTTCTTGGAACCTCC CGACTTGC
|-
|QR 207 || : ATATAATGTAATTACTAGAAAAAG CCTGTTTA
|-
|QR 207DH || : ATATATCCTCTTTTGAGGAACAAGTTTTCTTGGATGTAATTACT AGAAAAAG CCTGTTTA
|-
|QR 208 || : TTTTTGCGTGCGTTATACAA
ATTCTCTAAGAA
|-
|QR 208DH || : TTTTTGCGTGCGTTATACAAATTCTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAGAA
|-
|QR 208'DH || : GTATCATATGCGTTATACAA
ATTCTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAGAA
|-
|QR 209 || : GAATCTTAAAGGCTTATCCGGTATTTACCAGT
|-
|QR 210 || : TACCTTTAAACGCTCAACAGTAGGAGCAAATC
|-
|QR 210DH || : TACCTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTAAACGCTCAACAGTAGGAGCAAATC
|-
|QR 210' || : ATAAAGCCAACGCTCAACAGTAGGAGCAAATC
|-
|QR 211 || : GAGCCTAAACCGCGCCCAATAGCAGCTTAATT
|-
|QR 211DH || : GAGCCTAAACCGCGCCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAATAGCAGCTTAATT
|-
|QR 212 || : GAAGCAAACCATATTTAACAACGCCATCGTAG
|-
|QR 212DH || : GAGAAT
CGCCATATTTAACAACGCCATCTCCTCTTTTGAGGAACAAGTTTTCTTGGGTAG
|-
|QR 212'DH || : GAGAAT
CGCCATATTTAACAACGCCATCTCCTCTTTTGAGGAACAAGTTTTCTTGGGTAG
|-
|QR 213 || : CATATTATAAGCCGTTTTTATTTTCAACATGT
|-
|QR 214 || : GAACAAGCTTATCCCAATCCAAATAAGAAACG
|-
|QR 214DH || : GAACATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCTTATCCCAATCCAAATAAGAAACG
|-
|QR 215 || : TTTTAATTCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 215DH || : TTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 215' || : AATTTAGCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 216 || : ATTTTTTGTAAACCAAGTACCGCACCAGTAAT
|-
|QR 216DH || : ATTTTTTGTAAACCAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGTACCGCACCAGTAAT
|-
|QR 217DH || : AAGAGGAAATAAAGTACCGACAAATTCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAGA
|-
|QR 217'DH || : AAGAGAATATAAAGTACCGACAAATTCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAGA
|-
|QR 218 || : AGCCTTTACTGTCTTTCCTTATCAAGGTAAAG
|-
|QR 219 || : TCAGAAGCTCCAGACGACGACAATACCAATCA
|-
|QR 219DH || : TCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCTC
CAGACGACGACAATACCAATCA
|-
|QR 219' || : TAATTCTGTC
CAGACGACGACAATACCAATCA
|-
|QR 220 || : GAAGCGCA TTACGAGCATGTAGAAAAACAACA
|-
|QR 220DH || : GAAGCGCATTACGAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGTGTAGAAAAACAACA
|-
|QR 221 || : CAAAAATCTAATGCAGAACGCGCC AATATCCC
|-
|QR 221' || : TGTTCAGCTAATGCAGAACGCGCCAATATCCC
|-
|QR 222 || : CCCTGAACCCTGAACAAGAAAAATTGTTTATC
|-
|QR 222' || : CCCTGAACCCTGAACAAGAAAAATTGTTTATCAACAATAG
|-
|QR 223DH || : CTCCGTGGCTTTCCGGCACCGCTTCTGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTGCC
|-
|QR 224 || : TTAAATGCAACAGGAAGA
TTGTATAAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAT
|-
|QR 224DH || : TTAAATGCAACAGGAAGA
TTGTATAAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAT
|}
 
</font>

Revision as of 21:28, 15 September 2014








Top Team Project Design Experiment
and Result 		protocol Sources Sponser

Protocols
・Materials Staples DNA were purchased from IDT ( U.S.A )


Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM.


↓


These solutions were taken 2 μL each and mixed.


↓

The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.


↓


The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added.


↓


We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS).


↓



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