Biomod/2014/Kansai/Sources: Difference between revisions

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<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Project|<font face="Comic Sans MS,cursive,Arial" color="white">Project</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Project|<font face="Comic Sans MS,cursive,Arial" color="white">Project</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Results|<font face="Comic Sans MS,cursive,Arial" color="black">Design</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Results|<font face="Comic Sans MS,cursive,Arial" color="black">Design</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Protocols|<font face="Comic Sans MS,cursive,Arial" color="white">Experiment <br>and Result</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Sources|<font face="Comic Sans MS,cursive,Arial" color="black">protocol</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Etc.|<font face="Comic Sans MS,cursive,Arial" color="white">Sources</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Etc.|<font face="Comic Sans MS,cursive,Arial" color="white">Sources</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/sponser|<font face="Comic Sans MS,cursive,Arial" color="black">Sponser</font>]] </td>
</tr>
</tr>
</table>
</table>


<br>
<Font Size="4">
<table border="1" cellpadding="4" width="1000" >
<tr align="center">
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/Protocols|<font face="Comic Sans MS,cursive,Arial" color="black">Experiment</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Experiment|<font face="Comic Sans MS,cursive,Arial" color="white">Further experiment</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/New sources|<font face="Comic Sans MS,cursive,Arial" color="black">Future plan</font>]] </td>
<td width="180" bgcolor="#000000">[[Biomod/2014/Kansai/Sources|<font face="Comic Sans MS,cursive,Arial" color="white">protocol</font>]] </td>
<td width="180" bgcolor="#FFFFFF">[[Biomod/2014/Kansai/sponser|<font face="Comic Sans MS,cursive,Arial" color="black">Sponser</font>]] </td>


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</tr>
'''・Staples for the components'''<br>
</table>


</div>
<font size="4">
'''Protocols'''<br>
・Materials
Staples DNA were purchased from IDT ( U.S.A )


<Font Size="2">
<br>
{|
Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM.
|-
<div align="center">[[image:1.JPG|450px]]</div>
|QR 1 || : TTCGCGTCAAAGCCTCAGAGCATAAGAATTAGCAAAATTA
<br>
|-
<div align="center"><font size="10">↓</font></div>
|QR 1' || : TTTTTAACACCAAAAACATTATGATTAACATC
<br>
|-
These solutions were taken 2 μL each and mixed.
|QR 2 || : TTAACATCCAATAAATCATACAGGAAGCTAAA
<div align="center">[[image:3.JPG|450px]]</div>
|-
<br>
|QR 2' || : TCAATTCTACTAATAGTAGTAGCACCCTGTAA
<div align="center"><font size="10">↓</font></div>
|-
The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.
|QR 3 || : TTTTTAACACCAAAAA CATTATGATTAACATC
<br>
|-
<div align="center">[[image:5.JPG|450px]]</div>
|QR 3DH || : TTTTTAACACCAAAAACATTATGATTAATCCTCTTTTGAGGAACAAGTTTTCTTGGCATC
<br>
|-
<div align="center"><font size="10">↓</font></div>
|QR 4DH || : TCAATTCTCCTCTTTTGAGGAACAAGTTTTCTTGGTACTAATAGTAGTAGCACCCTGTAA
<br>
|-
The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added.
|QR 4' || : CTACCTTTACTAATAGTAGTAGCACCCTGTAA
<div align="center">[[image:6.JPG|450px]]</div>
|-
<br>
|QR 5 || : ATTCGCATCGGGAGAAGCCTTTATAGGTGGCA
<div align="center"><font size="10">↓</font></div>
|-
<br>
|QR 5DH || : ATTC
TCCTCTTTTGAGGAACAAGTTTTCTTGGGCATCGGGAGAAGCCTTTATAGGTGGCA
We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS).
|-
<div align="center">[[image:IMG_1447.JPG|450px]]</div>
|QR 6DH || : TTTCATTTGGGGCGCGATCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGAAATTCAACGC
<br>
|-
<div align="center"><font size="10">↓</font></div>
|QR 6' || : ATATAACTGGGGCGCGAGCTGAAATTCAACGC
<br>
|-
<div align="center">[[image:ex8.png|450px]]</div>
|QR 6'DH || : ATATAACTGGGGCGCGATCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGAAATTCAACGC
<br>
|-
|QR 7 || : ATTTAAATAAATTTTTAGAACCCTAGCTATAT
|-
|QR 7DH || : ATTTAAATAAATTTTTAGAACCCTAGCTTCCTCTTTTGAGGAACAAGTTTTCTTGGATAT
|-
|QR 8DH || : TCGCATCCTCTTTTGAGGAACAAGTTTTCTTGGAATGGTCAATAACCTGTTTCATATATT
|-
|QR 8' || : ATTTAAATAAATTTTTAGAACCCTAGCTATAT
|-
|QR 9 || : GCCCCAAAAATGCCTGAGTAATGTGATACATT
|-
|QR 10DH || : CGAGTAGATTTAGTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCATTAGTAGGTAA
|-
|QR 10' || : TCAAATATTTTAGTTTGACCATTAGTAGGTAA
|-
|QR 10'DH || : TCAAATATTTTAGTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCATTAGTAGGTAA
|-
|QR 11 || : AATCATATAAGGGTGAGAAAGGCCTCTGCGAA
|-
|QR 11DH || : AATCATATAAGGGTGAGAAAGGCCTCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGAA
|-
|QR 12 || : TCCATATAACAGTTGATTCCCAATGGAGACAG
|-
|QR 12' || : CCTAAATTACAGTTGATTCCCAATGGAGACAG
|-
|QR 13 || : TCGATGAACCATCAATATGATATTCCATCAAT
|-
|QR 13DH || : TCGATTCCTCTTTTGAGGAACAAGTTTTCTTGGGAACCATCAATATGATATTAGTTTCAT
|-
|QR 14 || : TGCAACTAAAGTACGGTGTCTGGACAACCGTT
|-
|QR 14DH || : TGATAAATAAGTACGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCTGGACAACCGTT
|-
|QR 14'DH || : TGATAAATAAGTACGGTGTCTGGACAACCGTT
|-
|QR 15 || : ATATAATG CTGTAGCTCAACATGTTTTAAATA
|-
|QR 15DH || : CGGAATCACTGTAGCTCAACATGTTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 15'DH || : CGGAATCACTGTAGCTCAACATGTTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 16 || : AGGTAGAAAGATTCATCAGTTGAGATTGCTGA
|-
|QR 16DH || : AGGTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAAAGATTCATCAGTTGAGATTGCTGA
|-
|QR 17DH || : TTTTTGCGGATGGCTTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCTTAATTTAGGA
|-
|QR 17' || : GTATCATAGATGGCTTAGAGCTTAATTTAGGA
|-
|QR 17'DH || : GTATCATAGATGGCTTATCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCTTAATTTAGGA
|-
|QR 18 || : AATAAAACTTCAACTAATGCAGATAGAGGTCA
|-
|QR 18DH || : AATAAAACTTCAACTAATGCAGATAGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCA
|-
|QR 19 || : TACCTTTAATTGCTCCTTTTGATAACATAACG
|-
|QR 19' || : ATAAAGCCATTGCTCCTTTTGATAACATAACG
|-
|QR 20 || : CAGTCAGGAATTACGAGGCATAGTTTAGAGAG
|-
|QR 20DH || : CAGTCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGAATTACGAGGCATAGTTTAGAGAG
|-
|QR 21DH || : GAAGCAAACTCCAACAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTCAGGAAAGAGCAA
|-
|QR 21' || : GAGAAT
CGCTCCAACAGGTCAGGAAAGAGCAA
|-
|QR 22 || : CTTATGCGTAACCCTCGTTTACCACCAGACCG
|-
|QR 22DH || : CTTATGCGTAACCCTCGTTTACCACCAGTCCTCTTTTGAGGAACAAGTTTTCTTGGACCG
|-
|QR 23DH || : TTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTCGAGCTTCAAAGCGAAGACGACGA
|-
|QR 23' || : AATTTAGGCGAGCTTCAAAGCGAAGACGACGA
|-
|QR 24 || : GTTTAATTAAAATAGCGAGAGGCTATATCGCG
|-
|QR 24DH || : GTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTAAAATAGCGAGAGGCTATATCGCG
|-
|QR 25DH || : AAGAGGAAGCCCGAAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGACTTCAATTTGCAAA
|-
|QR 25' || : AAGAGAATGCCCGAAAGACTTCAATTTGCAAA
|-
|QR 26 || : ACCAGA
ACTGCCAGAGGGGGTAATAAAAGATT
|-
|QR 26DH || : ACCAGAACTGCCAGAGGGGGTAATAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGGATT
|-
|QR 27DH || : TCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 27' || : TAATTCTGAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 28 || : TTCAGTGATGGATAGCGTCCAATATATTATAG
|-
|QR 28DH || : TAATTCTGAAAGCGGATTGCATCAAGTAAAAT
|-
|QR 29DH || : CAAAAATCAGGTCTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGCCCTGACCTGCGGAA
|-
|QR 29' || : TGTTCAGCAGGTCTTTACCCTGACCTGCGGAA
|-
|QR 30 || : CATTACCCAATATTCATTGAATCCCCATAAAT
|-
|QR 31 || : CAGAAAACGAGAATGACCCTCAAA
|-
|QR 31' || : AACAATAGCAGAAAACGAGAATGACCCTCAAA
|-
|QR 32 || : GCTGCGCATCCTGTAGCCAGCTTTAAAAATAA
|-
|QR 33 || : TCGGTTGTCAATAGGAACGCCATCCATCAACA
|-
|QR 34DH || : GGAAACCAGAGCGAGTAACAACCCCAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGTCAT
|-
|QR 35 || : TACTTTTGTAAATTTTTGTTAAATGTCGGATT
|-
|QR 36 || : CCAGCCAGGAACAAACGGCGGATTTTGTTAAA
|-
|QR 36DH || : CCAG
TCCTCTTTTGAGGAACAAGTTTTCTTGGCCAGGAACAAACGGCGGATTTTGTTAAA
|-
|QR 37 || : AAGGATAATGTAAACGTTAATATTGACCGTAA
|-
|QR 38 || : CGTCACCCTCAGCAGCGAAAGACATCAGAAAA
|-
|QR 38DH || : CGTCATCCTCTTTTGAGGAACAAGTTTTCTTGGCCCTCAGCAGCGAAAGACATCAGAAAA
|-
|QR 39 || : AGATTCAAGTACCCCGGTTGATAAGCATCGGA
|-
|QR 40DH || : TTGCAGGGAGCAACGGCTACAGAGAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGTGTC
|-
|QR 41 || : TCAAATCACGGTAATCGTAAAACTGCTTTGAG
|-
|QR 42 || : CACGCATAACTTTTTCATGAGGAACAAGAGAA
|-
|QR 42DH || : CTAGCTGAGAGAGT
CTGGAGCAAAGTTTCCAT
|-
|QR 43 || : CTAGCTGAGAGAGT
CTGGAGCAAAGTTTCCAT
|-
|QR 43DH || : CTAGCTGAGAGAGTCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAGCAAAGTTTCCAT
|-
|QR 44 || : CATTGCCTTAAATTAATGCCGGAGAGGGTAGC
|-
|QR 45DH || : TATTTTTGAGAGATCTACAAAGGCTATCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGT
|-
|QR 46 || : CGAGGTGACCTAAAACGAAAGAGGATTATTAC
|-
|QR 46DH || : CGAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTGACCTAAAACGAAAGAGGATTA
TTAC
|-
|QR 47 || : ATACCACAGAACTAACGGAACAACCAAAAGAA
|-
|QR 47DH || : ATACCACAGAACTAACGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAACAACCAAAAGAA
|-
|QR 48 || : CCTTTAATAACACTCATCTTTGACTCTACGTT
|-
|QR 48DH || : CCTTTAATAACACTCATCTTTGACTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGCGTT
|-
|QR 49 || : CCAAAAGGACGTTGGGAAGAAAAACCCCAGCG
|-
|QR 50 || : GAAAATCTAAGCGCGAAACAAAGTCATTATAC
|-
|QR 50DH || : GAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCTAAGCGCGAAACAAAGTCATTATAC
|-
|QR 51 || : CACTATCAATTTTAAGAACTGGCTACAACGGA
|-
|QR 51DH || : CACTATCAATTTTAAGATCCTCTTTTGAGGAACAAGTTTTCTTGGACTGGCTACAACGGA
|-
|QR 52 || : AAAGGAATTCATCGCCTGATAAATTGAATTAC
|-
|QR 52DH || : AAAGGAATTCATCGCCTGATAAAT
TGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTAC
|-
|QR 53 || : TAAAAACCTCAACTTTAATCATTGTGTGTCGA
|-
|QR 54 || : TTTCAGCGACCTGCTCCATGTTACTTGAGATG
|-
|QR 54DH || : TTTCATCCTCTTTTGAGGAACAAGTTTTCTTGGGCGACCTGCTCCATGTTACTTGAGATG
|-
|QR 55 || : AGAAGTTTGAGTAGTAAATTGGGCTTAGCCGG
|-
|QR 56 || : TGTATGGGGCAGACGGTCAATCATCGAGAAAC
|-
|QR 56DH || : TGTATG
GGGCAGACGGTCAATCATCGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAC
|-
|QR 57 || : GTTTAGACATAAGGCTTGCCCTGAAAGGGAAC
|-
|QR 58 || : TCGTCTTTCCAACTTTGAAAGAGGGCTGCTCA
|-
|QR 58DH || : TCGTCTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTCCAACTTTGAAAGAGGGCTGCTCA
|-
|QR 59 || : TCGTCATAAAATCAACGTAACAAAACAGATGA
|-
|QR 59DH || : TCGTCATAAAATCAACGTCCTCTTTTGAGGAACAAGTTTTCTTGGTAACAAAACAGATGA
|-
|QR 60 || : CCTCATAGCAGACCAGGCGCATAGCGGATATT
|-
|QR 61 || : TGCTTTAATAATCTTGACAAGAACGCTGGCTG
|-
|QR 62 || : ACGGCCAGTGGGAAGGGCGATCGGCCATTCAG
|-
|QR 63 || : TTAAATGTGGCAAAGCGCCATTCGTGCGGGCC
|-
|QR 64 || : CCTCATTAAAGCCAGAATGGAAAGATCGCACT
|-
|QR 64DH || : CCTCTCCTCTTTTGAGGAACAAGTTTTCTTGGATTAAAGCCAGAATGGAAAGATCGCACT
|-
|QR 65DH || : TGGGATAGTATCGGCCTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGGAAGCGCAGTCT
|-
|QR 66 || : GACGACAGGTCACGTTGGTGTAGATGGGCGCA
|-
|QR 67 || : TCGTAACCGTGCATCTGC
CAGTTTGAGGGGAC
|-
|QR 67DH || : TCGTAACCGTGCATCTGCCAGTTTGAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGGAC
|-
|QR 68 || : CCAGCATTATGATACAGGAGTGTATGCGGGAT
|-
|QR 68DH || : CCAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGATTATGATACAGGAGTGTATGCGGGAT
|-
|QR 69 || : ACGAGGGTAGTTAAAGGCCGCTTTCTGGTAAT
|-
|QR 70 || : AGCCGCCAACGGGGTCAGTGCCTTGCTGAGGC
|-
|QR 70DH || : AGCCGCCAACGGGGTCAGTGCCTTGCTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGGC
|-
|QR 71 || : GACTAAAGACCGATATATTCGGTCGAGTAACA
|-
|QR 72 || : AGAACCGCATAAACAGTTAATGCCCCATCGCC
|-
|QR 72DH || : AGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGCGCATAAACAGTTAATGCCCCATCGCC
|-
|QR 73 || : TAAACGGGCCGACAATGACAACAACCCTGCCT
|-
|QR 73DH || : TAAACGGGCCGACAATGTCCTCTTTTGAGGAACAAGTTTTCTTGGACAACAACCCTGCCT
|-
|QR 74 || : TAGTTGCGTAAAATACGTAATGCCACTACGAA
|-
|QR 75 || : CCGGAACCACCTATTATTCTGAAAGATACCGA
|-
|QR 75DH || : CCGGAACCACCTATTATTCTGAAAGATATCCTCTTTTGAGGAACAAGTTTTCTTGGCCGA
|-
|QR 76 || : GGCACCAAATTTCTTAAACAGCTTCATGAAAG
|-
|QR 77 || : CATAATCAGGCTGAGACTCCTCAACTTGCTTT
|-
|QR 77DH || : CATAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCAGGCTGAGACTCCTCAACTTG
CTTT
|-
|QR 78 || : TACACTAATGTATCGGTTTATCAGGAGAAGGA
|-
|QR 78DH || : TACACTAATGTATCGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCAGGAGAAGGA
|-
|QR 79 || : CATAGCCCAGCGGGGTTTTGCTCAAAAAGGAG
|-
|QR 79DH || : CATAGCCCAGCGGGGTTTTGCTCAAAAATCCTCTTTTGAGGAACAAGTTTTCTTGGGGAG
|-
|QR 80 || : ATTATACCCCAAAAAAAAGGCTCCGTACCAGG
|-
|QR 81 || : GACTGTAGTGCCGTCGAGAGGGTTTTCACGTT
|-
|QR 82DH || : GATTTGTATGCGAATAATCCTCTTTTGAGGAACAAGTTTTCTTGGTAATTTTGATATAAG
|-
|QR 83 || : TAGCGACAGGAATAGGTGTATCACGAACAACT
|-
|QR 83DH || : TAGCGACAGGAATAGGTGTATCACGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGAACT
|-
|QR 84 || : AATCCGCGGAGTGAGAATAGAAAGCGTACTCA
|-
|QR 85 || : AATGAAACAGTACCGCCACCCTCATTCAACAG
|-
|QR 85DH || : AATGATCCTCTTTTGAGGAACAAGTTTTCTTGGAACAGTACCGCCACCCTCATTCAACAG
|-
|QR 86 || : AACGAGGCATTTTGCTAAACAACTGAACCGCC
|-
|QR 86DH || : AACGAGGCATTTTGCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAACAACTGAACCGCC
|-
|QR 87DH || : CCATTACCAACCGCCACCCTCAGAGAATTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTC
|-
|QR 88 || : CGAACTGACCAGACGTTAGTAAATGCCACCAC
|-
|QR 89 || : GGGAATTATCAGGGATAGCAAGCCAAGTTTTG
|-
|QR 89DH || : GGGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCAGGGATAGCAAGCCAAGTTTTG
|-
|QR 90 || : ACGGTGTATTAGCGTAACGATCTACAATAGGA
|-
|QR 90DH || : ACGGTGTATTAGCGTAATCCTCTTTTGAGGAACAAGTTTTCTTGGCGATCTACAATAGGA
|-
|QR 91 || : TGAATTATACCGTAACACTGAGTTCAGACAGC
|-
|QR 92 || : ACCTTCATCGCCTGTAGCATTCCATCGTCACC
|-
|QR 93 || : GACAGGAAGCTTGCATGCCTGCAGGTAAAACG
|-
|QR 94 || : TCTTCGCTTCCCAGTCACGACGTTGTCGACTC
|-
|QR 95 || : CAGGGTTTATTACGCCAGCTGGCGAAAGGGGG
|-
|QR 96 || : AGCTAAACCCCCGGGTACCGAGCTCGAATTCG
|-
|QR 97 || : ATGTGCTGCAAGGCGATTAAGTTGGGTAACGC
|-
|QR 97DH || : ATGTGCTGCAAGGCGATTAAGTTGGGTATCCTCTTTTGAGGAACAAGTTTTCTTGGACGC
|-
|QR 98 || : TAACGTGCGTCATAGCTGTTTCCTAAATAAAT
|-
|QR 98DH || : TAACTCCTCTTTTGAGGAACAAGTTTTCTTGGGTGCGTCATAGCTGTTTCCTAAATAAAT
|-
|QR 99DH || : CTGAATTTCTTGATATTTCCTCTTTTGAGGAACAAGTTTTCTTGGCACAAACGTGTGAAA
|-
|QR 100 || : CGATTGGCACCGTTCCAGTAAGCGTCATACAT
|-
|QR 101DH || : GCGCGTACCGCTCACAATTCCACAAGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGA
|-
|QR 102 || : GGCTTTTGGACAGGAGGTTGAGGCCAACATAC
|-
|QR 103DH    || : CACACTCCTCTTTTGAGGAACAAGTTTTCTTGGCCGAGCATAAAGTGTAAAGAGCCGCCG
|-
|QR 104 || : AAGTTTTACCAGAACCACCACCAGCCTGGGGT
|-
|QR 105DH || : GCAAGTGTAGTGAGCTAACTCACAACCCTCCTCTTTTGAGGAACAAGTTTTCTTGGTCAG
|-
|QR 106 || : GTGCCCGTCACCCTCAGAGCCACCTTAATTGC
|-
|QR 107 || : AGAAAGCGCACTGCCCGCTTTCCACCACCCTC
|-
|QR 108 || : ATTTCGGAGCCTCCCTCAGAGCCGGTCGGGAA
|-
|QR 108DH || : ATTTCGGAGCCTCCCTCTCCTCTTTTGAGGAACAAGTTTTCTTGGAGAGCCGGTCGGGAA
|-
|QR 109 || : AAAGCCGGTGCCAGCTGCATTAATAGCCACCA
|-
|QR 109DH || : AAAGCCGGTGCCAGCTGCATTAATAGCCTCCTCTTTTGAGGAACAAGTTTTCTTGGACCA
|-
|QR 110 || : TATTAAGAAAATCACCGGAACCAGGAATCGGC
|-
|QR 111 || : AAAGGGAGGGGGAGAGGCGGTTTGCATCTTTT
|-
|QR 111DH || : AAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGAGGGGGAGAGGCGGTTTGCATCTTTT
|-
|QR 112 || : TTAGGATTCCTTATTAGCGTTTGCCGTATTGG
|-
|QR 112DH || : TTAGGATTCCTTATTAGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGTTTGCCGTATTGG
|-
|QR 113 || : CGAGGTGCGTGGTTTTTCTTTTCATTTTCGGT
|-
|QR 113DH || : CGAGGTGCGTGGTTTTTCTTTTCATTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGT
|-
|QR 114 || : CGGATAAGCGCGTTTTCATCGGCACCAGTGAG
|-
|QR 115 || : TGAACCATCAGCTGATTGCCCTTCTAGCGTCA
|-
|QR 115DH || : TGAACTCCTCTTTTGAGGAACAAGTTTTCTTGGCATCAGCTGATTGCCCTTCTAGCGTCA
|-
|QR 116 || : TATAGCCCGAATCAAGTTTGCCTTACCGCCTG
|-
|QR 116DH || : TATAGCCCGAATCAAGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTGCCTTACCGCCTG
|-
|QR 117 || : AAAACCGTAGAGTTGCAGCAAGCGGTAATCAG
|-
|QR 117DH || : AAAACCGTAGAGTTGCAGCAAGCGGTAATCCTCTTTTGAGGAACAAGTTTTCTTGGTCAG
|-
|QR 118 || : GGAGGTTTCATCGATAGCAGCACCGTCCACGC
|-
|QR 119DH || : TAAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAACCCCAGCAGGCGAAAATACGTCACC
|-
|QR 120DH || : ACCCTCAGATTAGCAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGCCGGAACCTGTTTG
|-
|QR 121DH || : TGTTGTTCTCCGAAATCGGCAAAACAGTTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCA
|-
|QR 122 || : CCTCATTTGAGCCAGCAAAATCACTCCCTTAT
|-
|QR 123DH || : ACCCATGTCACCGTCACTCCTCTTTTGAGGAACAAGTTTTCTTGGCGACTTGAGCCATTT
|-
|QR 124 || : CACCACGGGGCGACATTCAACCGAATTAAAGG
|-
|QR 125 || : AGTACAAATTGACGGAAATTATTCTTGAGGGA
|-
|QR 126 || : AAAAGTTTCGCCAGAATCCTGAGAGGATTTTA
|-
|QR 127 || : TAGAGGATAGGAGGCCGATTAAAGAGTGTTTT
|-
|QR 128 || : ATTAAATCGTGAGGCCACCGAGTAAGAGCGGG
|-
|QR 128DH || : ATTAAATCGTGAGGCCACCGAGTAAGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGG
|-
|QR 129 || : TAATCATGTTTCCTCGTTAGAATCAAAGAGTC
|-
|QR 130 || : TATTAGACACGCAAATTAACCGTTAGCACGTA
|-
|QR 130DH || : TATTTCCTCTTTTGAGGAACAAGTTTTCTTGGAGACACGCAAATTAACCGTTAGCACGTA
|-
|QR 131 || : TTGTTATCTATGGTTGCTTTGACGGTAGCAAT
|-
|QR 131DH || : TTGTTATCTATGGTTGCTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTGACGGTAGCAAT
|-
|QR 132DH || : GTCAATAGGATTAGTAATAACATCGCTATCCTCTTTTGAGGAACAAGTTTTCTTGGCAGG
|-
|QR 133 || : GAGCCGGACCGCGCTTAATGCGCCACTTGCCT
|-
|QR 134 || : TTAGGAGCGAACTCAAACTATCGGGTAACCAC
|-
|QR 134DH || : TTAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCGAACTCAAACTATCGGGTAACCAC
|-
|QR 135 || : GCCTAATGAGCGGTCACGCTGCGCCCTTGCTG
|-
|QR 135DH || : GCCTAATGAGCGGTCACTCCTCTTTTGAGGAACAAGTTTTCTTGGGCTGCGCCCTTGCTG
|-
|QR 136 || : AAAGGAATCAGAACAATATTACCGGGGCGCTG
|-
|QR 136DH || : AAAGGAATCAGAACAATATTACCGGGGCTCCTCTTTTGAGGAACAAGTTTTCTTGGGCTG
|-
|QR 137 || : GTTGCGCTAAAGGAGCGGGCGCTACCAGCCAT
|-
|QR 138 || : CAATATCTGAAAAACGCTCATGGAGGAAGGGA
|-
|QR 139 || : ACCTGTCGCGAACGTGGCGAGAAAAATACCTA
|-
|QR 139DH || : ACCTGTCGCGAACGTGGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGAGAAAAATACCTA
|-
|QR 140 || : CCTTGCTGCGCTCAATCGTCTGAATGACGGGG
|-
|QR 140DH || : CCTTGCTGCGCTCAATCGTCTGAATGACTCCTCTTTTGAGGAACAAGTTTTCTTGGGGGG
|-
|QR 141 || : CAACGCGCCCCCCGATTTAGAGCTATGGATTA
|-
|QR 142 || : AGCCAGCAGGCAGATTCACCAGTCGGAACCCT
|-
|QR 142DH || : AGCCATCCTCTTTTGAGGAACAAGTTTTCTTGGGCAGGCAGATTCACCAGTCGGAACCCT
|-
|QR 143 || : GCGCCAGGCGTAAAGCACTAAATCACACGACC
|-
|QR 143DH || : GCGCCAGGCGTAAAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGCTAAATCACACGACC
|-
|QR 144 || : ATTAACACAAGGGACATTCTGGCCTTTGGGGT
|-
|QR 144DH || : ATTAACACAAGGGACATTCTGGCCTTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGGGGT
|-
|QR 145 || : ACGGGCAACACCCAAATCAAGTTTAACAGAGA
|-
|QR 146 || : GCAGAAGATTCTGACCTGAAAGCGCCACTACG
|-
|QR 146DH || : GCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGATTCTGACCTGAAAGCGCCACTACG
|-
|QR 147 || : GCCCTGAGCTATCAGGGCGATGGCTAAGAATA
|-
|QR 147DH || : GCCCTGAGCTATCAGGGTCCTCTTTTGAGGAACAAGTTTTCTTGGCGATGGCTAAGAATA
|-
|QR 148 || : CATCGCCAAGACAATATTTTTGAAAAGGGCGA
|-
|QR 148DH || : CATCGCCAAGACAATATTTTTGAAAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGGCGA
|-
|QR 149 || : TGGTTTGCGTGGACTCCAACGTCATGGCTATT
|-
|QR 150DH || : ATGGTGGTCAGTTTGGATCCTCTTTTGAGGAACAAGTTTTCTTGGACAAGAGTCCACTAT
|-
|QR 151 || : ATAGCTATAGGAAACGCAATAATAGGGTTGAG
|-
|QR 151DH || : ATAGCTATAGGAAACGCAATAATAGGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGTGAG
|-
|QR 152 || : AAATCAAAAGAATAGCCCGAGATAACGGAATA
|-
|QR 153 || : ATAGAAAATTCATATGGTTTACCAGCGCCAAA
|-
|QR 153DH || : ATAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAAATTCATATGGTTTACCAGCGCCAAA
|-
|QR 154 || : GACAAAAGAATAAGTTTATTTTGTCACAATCA
|-
|QR 154DH || : GACAAAAGAATAAGTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGATTTTGTCACAATCA
|-
|QR 155 || : AGAGAGATTATGTTAGCAAACGTACGCAAAGA
|-
|QR 156 || : GGGAAGGTCAACATATAAAAGAAAGAAAATAC
|-
|QR 157 || : TAGCGATACATTATCATTTTGCGGTTAATTTT
|-
|QR 158 || : TATAATCACTTTGCCCGAACGTTAAACAAAGA
|-
|QR 159 || : TAATTAATGAAGGAGCGGAATTATCAACTCGT
|-
|QR 159DH || : TAATTAATGAAGGAGCGGAATTATCAACTCCTCTTTTGAGGAACAAGTTTTCTTGGTCGT
|-
|QR 160 || : TGTCCATCTTTACAAACAATTCGACATCATAT
|-
|QR 161 || : TGAATAACATCAGATGATGGCAATTTTAGAAG
|-
|QR 161DH || : TGAATCCTCTTTTGAGGAACAAGTTTTCTTGGTAACATCAGATGATGGCAATTTTAGAAG
|-
|QR 162 || : ACTTCTTTATAATACATTTGAGGATCATCAAT
|-
|QR 163 || : ATGGAAACGATTGTTTGGATTATATTAGAGCC
|-
|QR 163DH || : ATGGAAACGATTGTTTGGATTATATTAGTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCC
|-
|QR 164 || : GAGTAGAAACTAACAACTAATAGACTTCTGAA
|-
|QR 165 || : ATTTAACAGGGTTAGAACCTACCAAAATATCT
|-
|QR 165DH || : ATTTATCCTCTTTTGAGGAACAAGTTTTCTTGGACAGGGTTAGAACCTACCAAAATATCT
|-
|QR 166 || : GTAATATCTGAGGAAGGTTATCTATATCAAAA
|-
|QR 166DH || : GTAATATCTGAGGAAGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTTATCTATATCAAAA
|-
|QR 167DH || : GAAACAAAACGTAAAACAGAAATAAACATCCTCTTTTGAGGAACAAGTTTTCTTGGGTTG
|-
|QR 168 || : TGCAACAGGGTCAGTTGGCAAATCAAGAAATT
|-
|QR 169 || : TCATTTCATTTCAGGTTTAACGTCCCCTCAAT
|-
|QR 170DH || : CATTTTGAAACCTCAAATCCTCTTTTGAGGAACAAGTTTTCTTGGTATCAAAAGATGAAT
|-
|QR 171 || : CCAAGTTAACAGTACCTTTTACATAAGCATCA
|-
|QR 172 || : TTTACATTGCAAATGAAAAATCTACGGGAGAA
|-
|QR 173 || : AGTAATAACGCCTGCAACAGTGCC ACGCTGAG
|-
|QR 173DH || : AGTAATAACGCCTGCAATCCTCTTTTGAGGAACAAGTTTTCTTGGCAGTGCCACGCTGAG
|-
|QR 174 || : CGCGAGGCTGCACCCAGCTACAATCGGTCAGT
|-
|QR 174DH || : CGCGAGGCTGCACCCAGCTACAATCGGTTCCTCTTTTGAGGAACAAGTTTTCTTGGCAGT
|-
|QR 175 || : TAGAACCCTAAAACAGAGGTGAGGTTTATCCT
|-
|QR 176 || : AGATATAGCCAACGCTAACGAGCGAACCACCA
|-
|QR 177 || : CGTGGCACTTAAAAATACCGAACGTCTTTCCA
|-
|QR 177DH || : CGTGGCACTTAAAAATATCCTCTTTTGAGGAACAAGTTTTCTTGGCCGAACGTCTTTCCA
|-
|QR 178 || : GAATCATTTTTGCCAGTTACAAAACCCTAAAA
|-
|QR 179 || : AGTCTTTAATGCGCGAACTGATAGTAAACAGC
|-
|QR 180 || : AGTAAGCAGATAGCCGAACAAAGTTACCAGAA
|-
|QR 181 || : GGAAACCGCTTACCGAAGCCCTTTTTAAGAAA
|-
|QR 182 || : ACGGGTATTTTAACGTCAAAAATGAAATAGCA
|-
|QR 182DH || : ACGGGTATTTTAACGTCAAAAATGAAATTCCTCTTTTGAGGAACAAGTTTTCTTGGAGCA
|-
|QR 183 || : CCCAAAAGAGAGCAAGAAACAATGAAAATAGC
|-
|QR 184 || : CAATAATAAACTGGCATGATTAAGACTCCTTA
|-
|QR 185 || : ATAATCGGCAGAGAGAATAACATAGTTAAGCC
|-
|QR 185DH || : ATAATTCCTCTTTTGAGGAACAAGTTTTCTTGGCGGCAGAGAGAATAACATAGTTAAGCC
|-
|QR 186 || : TTACGCAGAACCCACAAGAATTGAAAAACAGG
|-
|QR 186DH || : TTACGCAGAACCCACAATCCTCTTTTGAGGAACAAGTTTTCTTGGGAATTGAAAAACAGG
|-
|QR 187 || : ATCCTAATTTAGACGGGAGAATTACTAATATC
|-
|QR 188 || : ATACATAAAGAGGGTAATTGAGCGACTGAACA
|-
|QR 189 || : CAAAATTAATTAAGACGCTGAGAATGAAAACA
|-
|QR 189' || : ATTAAGACGCTGAGAATGAAAACA
|-
|QR 190 || : AACCACCATTTCCCTTAGAATCCTGAGTCAAT
|-
|QR 191 || : CAATAAATTATCAAAATCATAGGTGTCGCTAT
|-
|QR 191DH || : CAATAAATTATCAAAATCATAGGTGTCGTCCTCTTTTGAGGAACAAGTTTTCTTGGCTAT
|-
|QR 191'DH || : AGTGAATTTATCAAAATCATAGGTGTCGTCCTCTTTTGAGGAACAAGTTTTCTTGGCTAT
|-
|QR 192 || : TCCTGATTCTTGCTTCTGTAAATCCTGAGAGA
|-
|QR 193 || : TCAATTCTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 193DH || : TCAATCCTCTTTTGAGGAACAAGTTTTCTTGGTTCTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 193' || : CTACCTTTTTAACCTCCGGCTTAGTATGTGAG
|-
|QR 194 || : ATAATCCTAGTACATAAATCAATAGTTGGGTT
|-
|QR 194DH || : ATAATCCTAGTACATAATCCTCTTTTGAGGAACAAGTTTTCTTGG ATCAATAGTTGGGTT
|-
|QR 195 || : TTTCATTTATAT
GTAAATGCTGATCTTTTTTA
|-
|QR 195DH || : TTTCATTTATAT
GTAAATGCTGATCTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTA
|-
|QR 195'DH || : ATATAACTATAT
GTAAATGCTGATCTTTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTTA
|-
|QR 196 || : TAATGGAAATTTCATTTGAATTACGCAAATCC
|-
|QR 197 || : TCGCAAATGACAAAGAACGCGAGATTAATTAC
|-
|QR 197DH || : TCGCATCCTCTTTTGAGGAACAAGTTTTCTTGGAATGACAAAGAACGCGAGATTAATTAC
|-
|QR 197' || :AATCGCAAGACAAAGAACGCGAGATTAATTAC
|-
|QR 198 || : TTATTTGCCATCAAGAAAACAAAAAAACTT
TT
|-
|QR 198DH || : TTATTTGCCATCAAGAATCCTCTTTTGAGGAACAAGTTTTCTTGG AACAAAAAAACTT
TT
|-
|QR 199 || : CGAGTAGAATTTTAGTTAATTTCA AAGATGAT
|-
|QR 199DH || : CGAGTAGAATTTTAGTTAATTTCAAAGATCCTCTTTTGAGGAACAAGTTTTCTTGG TGAT
|-
|QR 199'DH || : TCAAATATATTTTAGTTAATTTCAAAGATCCTCTTTTGAGGAACAAGTTTTCTTGGTGAT
|-
|QR 200 || : GCGTAGATATTACCTGAGCAAAAGTCTTCTGA
|-
|QR 201 || : TCCATATATAATGGTTTGAAATACCGAATTAT
|-
|QR 201' || : CCTAAATTTAATGGTTTGAAATACCGAATTAT
|-
|QR 202DH || : ATACAGTACAAAATCGCTCCTCTTTTGAGGAACAAGTTTTCTTGG GCAGAGGCGACCGTG
|-
|QR 203 || : TGCAACTAAAGGCGTTAAATAAGATTTGAATA
|-
|QR 203DH || : TGCAACTAAAGGCGTTAAATAAGATTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 203'DH || : TGATAAATAAGGCGTTAAATAAGATTTGTCCTCTTTTGAGGAACAAGTTTTCTTGGAATA
|-
|QR 204 || : ACAATAACGGATTCGCCTGATTGCATAAACAC
|-
|QR 205 || : GGGAGGTTTTGAAGCCTTAAATCA AGATTAGT
|-
|QR 205DH || : GGGAGTCCTCTTTTGAGGAACAAGTTTTCTTGGGTTTTGAAGCCTTAAATCA AGATTAGT
|-
|QR 206 || : TGCTATTTGTTTTAGCGAACCTCC CGACTTGC
|-
|QR 206DH || : TGCTATTTGTTTTAGCGTCCTCTTTTGAGGAACAAGTTTTCTTGGAACCTCC CGACTTGC
|-
|QR 207 || : ATATAATGTAATTACTAGAAAAAG CCTGTTTA
|-
|QR 207DH || : ATATATCCTCTTTTGAGGAACAAGTTTTCTTGGATGTAATTACT AGAAAAAG CCTGTTTA
|-
|QR 208 || : TTTTTGCGTGCGTTATACAA
ATTCTCTAAGAA
|-
|QR 208DH || : TTTTTGCGTGCGTTATACAAATTCTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAGAA
|-
|QR 208'DH || : GTATCATATGCGTTATACAA
ATTCTCTATCCTCTTTTGAGGAACAAGTTTTCTTGGAGAA
|-
|QR 209 || : GAATCTTAAAGGCTTATCCGGTATTTACCAGT
|-
|QR 210 || : TACCTTTAAACGCTCAACAGTAGGAGCAAATC
|-
|QR 210DH || : TACCTTCCTCTTTTGAGGAACAAGTTTTCTTGGTTAAACGCTCAACAGTAGGAGCAAATC
|-
|QR 210' || : ATAAAGCCAACGCTCAACAGTAGGAGCAAATC
|-
|QR 211 || : GAGCCTAAACCGCGCCCAATAGCAGCTTAATT
|-
|QR 211DH || : GAGCCTAAACCGCGCCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAATAGCAGCTTAATT
|-
|QR 212 || : GAAGCAAACCATATTTAACAACGCCATCGTAG
|-
|QR 212DH || : GAGAAT
CGCCATATTTAACAACGCCATCTCCTCTTTTGAGGAACAAGTTTTCTTGGGTAG
|-
|QR 212'DH || : GAGAAT
CGCCATATTTAACAACGCCATCTCCTCTTTTGAGGAACAAGTTTTCTTGGGTAG
|-
|QR 213 || : CATATTATAAGCCGTTTTTATTTTCAACATGT
|-
|QR 214 || : GAACAAGCTTATCCCAATCCAAATAAGAAACG
|-
|QR 214DH || : GAACATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCTTATCCCAATCCAAATAAGAAACG
|-
|QR 215 || : TTTTAATTCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 215DH || : TTTTATCCTCTTTTGAGGAACAAGTTTTCTTGGATTCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 215' || : AATTTAGCAGAGGCATTTTCGAGCTCATCGA
|-
|QR 216 || : ATTTTTTGTAAACCAAGTACCGCACCAGTAAT
|-
|QR 216DH || : ATTTTTTGTAAACCAAGTCCTCTTTTGAGGAACAAGTTTTCTTGGTACCGCACCAGTAAT
|-
|QR 217DH || : AAGAGGAAATAAAGTACCGACAAATTCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAGA
|-
|QR 217'DH || : AAGAGAATATAAAGTACCGACAAATTCCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAGA
|-
|QR 218 || : AGCCTTTACTGTCTTTCCTTATCAAGGTAAAG
|-
|QR 219 || : TCAGAAGCTCCAGACGACGACAATACCAATCA
|-
|QR 219DH || : TCAGATCCTCTTTTGAGGAACAAGTTTTCTTGGAGCTC
CAGACGACGACAATACCAATCA
|-
|QR 219' || : TAATTCTGTC
CAGACGACGACAATACCAATCA
|-
|QR 220 || : GAAGCGCA TTACGAGCATGTAGAAAAACAACA
|-
|QR 220DH || : GAAGCGCATTACGAGCATCCTCTTTTGAGGAACAAGTTTTCTTGGTGTAGAAAAACAACA
|-
|QR 221 || : CAAAAATCTAATGCAGAACGCGCC AATATCCC
|-
|QR 221' || : TGTTCAGCTAATGCAGAACGCGCCAATATCCC
|-
|QR 222 || : CCCTGAACCCTGAACAAGAAAAATTGTTTATC
|-
|QR 222' || : CCCTGAACCCTGAACAAGAAAAATTGTTTATCAACAATAG
|-
|QR 223DH || : CTCCGTGGCTTTCCGGCACCGCTTCTGGTCCTCTTTTGAGGAACAAGTTTTCTTGGTGCC
|-
|QR 224 || : TTAAATGCAACAGGAAGA
TTGTATAAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAT
|-
|QR 224DH || : TTAAATGCAACAGGAAGA
TTGTATAAGCTCCTCTTTTGAGGAACAAGTTTTCTTGGAAAT
|}
 
</font>

Latest revision as of 00:54, 24 October 2014








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Experiment Further experiment Future plan protocol Sponser

Protocols
・Materials Staples DNA were purchased from IDT ( U.S.A )


Each staple DNA were dissolved by sterilized water that the concentration of each solution might be 100 μM.


↓


These solutions were taken 2 μL each and mixed.


↓

The volume of the solution was diluted with sterilized water to 500μL. we prepared staple DNA mixture.The sample (50 μM) was prepared mixing M13mp18 (4nM, Takara, Japan), staple DNA mixture (20nM) and 1×TAE/Mg2+ (12.5 mM). This mixture was kept at 90˚C for 10 minutes and cooled from 90˚C to 25˚C at a rate of -1.0˚C/min to anneal the strands.


↓


The annealed mixture (1μL) was deposited on freshly cleaved mica, additional 1X TAE/Mg2＋ buffer (40 µL) was added.


↓


We observed this product by AFM. the imaging was performed in the fluid Tapping mode with a BL-AC40TS tip (Olympus, Japan) and performed on a Multimode 8/ Nanoscope system (Bruker AVS).


↓



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