Biomod/2014/Kansai: Difference between revisions

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<h1 name="top" id="top">DNA nano QR code</h1>
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<span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Greetings!</strong></span></span></p>
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<span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">The NanoUANL team presents a model for synthesis of silver nanoparticle with specific dimensions within a viral capsid, using a semi-continuous tank reactor model  and single-enzyme confined kinetics. We also expand on the importance of mathematics in nanoscale design, and the limitations of computer analysis.</span></span></p>
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<span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Summary</strong></span></span></p>
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<p>&nbsp;Deoxyribonucleic acid (DNA) is greatly useful materials for a building block in constructing extremely small structure. DNA origami is constructed by combining complementary chains between a long circlic DNA strand (Scaffold DNA) and more 200 kinds of short strands (Staple DNA). It had been reported various nanostructures made of DNA origami such as smile mark and a star mark. Also it had been reported a DNA origami which is represented a character. But the quantity had not much. So, we tried to increase the amount of visually information. We focused on QR code that is one kind of two-dimensional code. It is possible to accommodate the large amount of data in a small space and not only numbers but also the language data such as English. Interestingly, QR code is read in a camera Apps. And it can read a little wrong QR code. In order to express it in nano size scale, we utilized height difference of DNA origami structure. Therefore, we used a dumbbell hairpin. It gives the height to DNA origami structure. Reading its structure in an AFM, nano QR code may be represented. We will design DNA origami by modifying dumbbell hairpins to arbitrary point. If camera App can read the figure of nano QR code made of the origami, our project will be complete! This will be a new information medium.</p>
<span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">An empty modified viral capsid, from the plant virus CCMV, was used as a container for horseradish peroxidase. Pores along the container permit only small molecules/particles to enter and exit the container, and flow is affected by an interior negative charge. Enzymatic activity with H202 releases electrons, which are accepted by silver ions in the solution. These ions are deposited when they are reduced from Ag (I) to Ag (0), and begin to nucleate, forming a nanoparticle within the inside of the container.</span></span></p>
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<span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Conclusion</strong></span></span></p>
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<span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">We succeded at modeling the nanoreactor, and were able to describe the diffusion through the pores of the capsid, and accumulation of the product on the interior.</span></span></p>
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Revision as of 02:43, 30 July 2014

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<html> <head> <title></title> </head> <body> <p> <span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Greetings!</strong></span></span></p> <p> <span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">The NanoUANL team presents a model for synthesis of silver nanoparticle with specific dimensions within a viral capsid, using a semi-continuous tank reactor model and single-enzyme confined kinetics. We also expand on the importance of mathematics in nanoscale design, and the limitations of computer analysis.</span></span></p> <p> &nbsp;</p> <p> <span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Summary</strong></span></span></p> <p> <span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">An empty modified viral capsid, from the plant virus CCMV, was used as a container for horseradish peroxidase. Pores along the container permit only small molecules/particles to enter and exit the container, and flow is affected by an interior negative charge. Enzymatic activity with H202 releases electrons, which are accepted by silver ions in the solution. These ions are deposited when they are reduced from Ag (I) to Ag (0), and begin to nucleate, forming a nanoparticle within the inside of the container.</span></span></p> <p> &nbsp;</p> <p> <span style="font-family:trebuchet ms,helvetica,sans-serif;"><span style="font-size: 16px;"><strong>Conclusion</strong></span></span></p> <p> <span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;">We succeded at modeling the nanoreactor, and were able to describe the diffusion through the pores of the capsid, and accumulation of the product on the interior.</span></span></p> <p> &nbsp;</p> <p style="text-align: center;"> <img alt="" height="90" src="http://openwetware.org/images/3/32/UANLHomeFCFM.jpg" width="92" /><span _fck_bookmark="1" style="display: none;">&nbsp;</span><img alt="" height="92" src="http://openwetware.org/images/9/9e/UANLHomeFCQ.jpg" width="94" /><span _fck_bookmark="1" style="display: none;">&nbsp;</span><span style="font-size:14px;"><span style="font-family: trebuchet ms,helvetica,sans-serif;"><img alt="" height="91" src="http://openwetware.org/images/9/97/UANLHomeFCB.jpg" width="91" /></span></span></p> </body> </html>

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