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| <html lang="ja">
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| <title>team hokudai</title>
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| body {
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| background-image: url(Team Hokudai.jpg);
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| p.example1 { font: 200% Times New Roman; }
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| p.example2 { text-align: center; }
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| </style>
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| </head>
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| <body>
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| <p class="example1">Process</p>
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| <p class="example6">
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| ① We prepared flow cells by placing a cover glass (18 × 18 mm2; MATSUNAMI) on an another slide glass (26 × 76 mm2) with a pair of double sided tapes as spacers. Size of flow cell: 1.5 × 18 × 0.1 mm3 (~3 µL) (W × L × H) in dimension.<br>
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| ② GTP-buffer and tubulin solution were mixed at the ratio of 1 : 4 (Final tubulin concentration: 112.5 µM).<br>
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| ③ Tubulin mixture was incubated at 4 °C for 10 minutes to depolymerize it completely. It was confirmed that polymerization of tubulin had not been started yet by using a polarizing microscopes.<br>
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| ④ The backside of the area of interest in the flow cell was fixed with a black tape where polymerization of tubulin would be initiated by IR irradiation. <br>
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| ⑤ A part of the flow cell where black tape was fixed was warmed for 15 minutes by using IR irradiation (IRS-001C; IR SYSTEM Co., Ltd., 5V / 1.6A).<br>
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| ⑥ Microtubules were observed by using fluorescence* microscope and polarizing microscope.<br>
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| *That time we used TAMRA labeled tubulin.
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| .</p>
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| <p><a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/f/f5/%E7%AD%8B%E8%82%89%E8%8B%B1%E8%AA%9E%E7%89%88.png" width="450" height="450" border="0" /></a>
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| <a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/8/86/%E7%B8%AE%E3%82%80%E3%82%B5%E3%83%AB%E3%82%B3%E3%83%A1%E3%82%A2.gif" width="400" height="300" border="0" /></a></p>
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| <p>(Figure1.Left:Structure of muscle,Right:contraction of sarcomeres)</p>
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| </div>
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| </body>
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| </html>
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