Biomod/2014/HKBUteam/methodology: Difference between revisions
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2.Design protocol | 2.Design protocol | ||
<b>Oligo list for staple strands and scaffold</b> | |||
1.All the oligo strands were ordered from TechDragon Limited, Hong Kong. | |||
2.Note: A 2,3,6 are modified by adding amine on 5’ end. | |||
3.Reviews of the DNA origami design | 3.Reviews of the DNA origami design | ||
Revision as of 06:55, 22 October 2014
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Methodology
DNA origami Design
1.DNA origami Structural requirements
DNA origami was designed using caDNAno software (http://cadnano.org/).
2.Design protocol
Oligo list for staple strands and scaffold
1.All the oligo strands were ordered from TechDragon Limited, Hong Kong.
2.Note: A 2,3,6 are modified by adding amine on 5’ end.
3.Reviews of the DNA origami design
Conjugation of the load
- Conjugation of DNA origami with folic acid
- Conjugation of DNA origami with chlorambucil
- Conjugation of DNA origami with graphene quantum dot
Assembly of DNA origami
- Self-assembly of DNA origami condition
- DNA origami purification
- Characterized DNA origami by atomic force microscope
Confocal microscopy & cytotoxicity assessment
- Confocal microscopy for graphene quantum dot DNA origami complex
- Cytotoxicity assessment by MTT assay for DNA origami drug delivery efficacy
