Biomod/2014/HKBUteam/experimentalresults: Difference between revisions
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Hep G2 Treatment | Hep G2 Treatment | ||
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Hep G2 Control | Hep G2 Control | ||
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Excitation : 405nm | Excitation : 405nm | ||
Revision as of 07:47, 24 October 2014
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1st Trial
Conjugation condition
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
1:1
RTP
Mixture was vortex for 4 hrs
In-Complete route
Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.
Mass-spectrometry
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil.
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid.
For MS-single stranded DNA A6 , a peak at 11171 shows the conjugation of A6 and carbon nanodots.
DNA extraction after folding of DNA origami
Gel photo :
Nanodrop Reading
DNA concentration : 0.8 ng/ul
260/280 : 1.34
260/230 : 0.86
Confocal Microscopy
Hep G2 Treatment
Hep G2 Control
Excitation : 405nm
Emission : 460 – 560nm
2nd Trial
Conjugation condition
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
1:1
RTP
Mixture was vortex for 4 hrs
In-Complete route
Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.
Mass-spectrometry
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid
DNA extraction after folding of DNA origami
Gel photo :
Nanodrop Reading
DNA concentration : 2.3 ng/ul
260/280 : 1.28
260/230 : 0.52
Confocal Microscopy
Hep G2 Treatment
Hep G2 Control
Excitation : 405nm
Emission : 460 – 560nm
3rd Trial
Conjugation condition
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
1:10
37oC
Mixture was vortex for 4 hrs
Complete route
All substituent was conjugated and folded simultaneously
Mass-spectrometry
For MS-single stranded DNA A2 , a peak at 10193 shows the conjugation of A2 and chlorambrucil
For MS-single stranded DNA A3 , a peak at 7043 shows the conjugation of A3 and folic acid
DNA extraction after folding of DNA origami
Gel photo :
Nanodrop Reading
DNA concentration : 5.5 ng/ul
260/280 : 2.65
260/230 : 1.12
Confocal Microscopy
Hep G2 Treatment
Hep G2 Control
HK-1 Treatment
HK-1 Control
Excitation : 405nm
Emission : 460 – 560nm
Emission Spectrum
Hep G2 HK-1
AFM photo
