Biomod/2014/HKBUteam/experimentalresults: Difference between revisions
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Conjugation condition | <b>Conjugation condition</b> | ||
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<h4>Mass-spectrometry</h4> | <h4>Mass-spectrometry</h4> | ||
[[Image:expresult1.jpg|190px]]<br> | |||
[[Image:expresult2.jpg|190px]]<br> | |||
[[Image:expresult3.jpg|190px]]<br> | |||
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil. | |||
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid. | |||
For MS-single stranded DNA A6 , a peak at 11171 shows the conjugation of A6 and carbon nanodots. | |||
DNA extraction after folding of DNA origami | <h4>DNA extraction after folding of DNA origami</h4> | ||
Gel photo : | |||
[[Image:expresult4.jpg|190px]]<br> | |||
Confocal Microscopy | <h4>Nanodrop Reading</h4> | ||
DNA concentration : 0.8 ng/ul | |||
260/280 : 1.34 | |||
260/230 : 0.86 | |||
<h4>Confocal Microscopy</h4> | |||
Hep G2 Treatment | |||
[[Image:expresult5.jpg|190px]][[Image:expresult6.jpg|190px]][[Image:expresult7.jpg|190px]]<br> | |||
Hep G2 Control | |||
[[Image:expresult8.jpg|190px]][[Image:expresult9.jpg|190px]][[Image:expresult10.jpg|190px]]<br> | |||
Excitation : 405nm | |||
Emission : 460 – 560nm | |||
==2nd Trial== | ==2nd Trial== |
Revision as of 09:05, 23 October 2014
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1st Trial
Conjugation condition
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
1:1
RTP
Mixture was vortex for 4 hrs
In-Complete route Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.
Mass-spectrometry
File:Expresult1.jpg
File:Expresult2.jpg
File:Expresult3.jpg
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil.
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid.
For MS-single stranded DNA A6 , a peak at 11171 shows the conjugation of A6 and carbon nanodots.
DNA extraction after folding of DNA origami
Gel photo :
File:Expresult4.jpg
Nanodrop Reading
DNA concentration : 0.8 ng/ul 260/280 : 1.34 260/230 : 0.86
Confocal Microscopy
Hep G2 Treatment
File:Expresult5.jpgFile:Expresult6.jpgFile:Expresult7.jpg
Hep G2 Control
File:Expresult8.jpgFile:Expresult9.jpgFile:Expresult10.jpg
Excitation : 405nm
Emission : 460 – 560nm
2nd Trial
Mass-spectrometry