Biomod/2014/HKBUteam: Difference between revisions
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=Project abstract= | =Project abstract= | ||
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Self-Assembly DNA origami, a breakthrough in Structural DNA nanotechnology by using hundreds of short ‘staple’ strands to mediate the folding of a long scaffold Single Stranded DNA from M13 phage genome to produce a variety of 2D and 3D nanostructures, have provided numerous possibilities for researchers to exploit for physical and biological applications. Despite a hollow DNA box has been used in diagnostic and therapeutic applications for fighting disease, however, such a design is often expensive and required a sophisticated mechanism to release the load. As an improved approach, our team has explored the possibility of design and preparation of a functionalized DNA origami. Built on the scaffold of the origami, we call for the conjugation with folic acid (a widely employed targeting agent for cancer cells), chlorambucil (an anticancer drug for chemotherapic action) and graphene quantum dot (a biocompatible fluorescent imaging agent) affording a potential theranostic device with targeting, diagnostic and therapeutic function. The fabricated “origami cargo” will be characterized by southern blot , TEM and fluorescence measurement. The cytotoxicity and cell uptake efficiency in living cancer cells will be explored using MTT assay and confocal microscopy. | Self-Assembly DNA origami, a breakthrough in Structural DNA nanotechnology by using hundreds of short ‘staple’ strands to mediate the folding of a long scaffold Single Stranded DNA from M13 phage genome to produce a variety of 2D and 3D nanostructures, have provided numerous possibilities for researchers to exploit for physical and biological applications. Despite a hollow DNA box has been used in diagnostic and therapeutic applications for fighting disease, however, such a design is often expensive and required a sophisticated mechanism to release the load. As an improved approach, our team has explored the possibility of design and preparation of a functionalized DNA origami. Built on the scaffold of the origami, we call for the conjugation with folic acid (a widely employed targeting agent for cancer cells), chlorambucil (an anticancer drug for chemotherapic action) and graphene quantum dot (a biocompatible fluorescent imaging agent) affording a potential theranostic device with targeting, diagnostic and therapeutic function. The fabricated “origami cargo” will be characterized by southern blot , TEM and fluorescence measurement. The cytotoxicity and cell uptake efficiency in living cancer cells will be explored using MTT assay and confocal microscopy. | ||
Revision as of 03:01, 25 October 2014
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Project Title
'TRINITY'---a potential origami-based theranostic agent in anti-cancer therapy.
Project abstract
Self-Assembly DNA origami, a breakthrough in Structural DNA nanotechnology by using hundreds of short ‘staple’ strands to mediate the folding of a long scaffold Single Stranded DNA from M13 phage genome to produce a variety of 2D and 3D nanostructures, have provided numerous possibilities for researchers to exploit for physical and biological applications. Despite a hollow DNA box has been used in diagnostic and therapeutic applications for fighting disease, however, such a design is often expensive and required a sophisticated mechanism to release the load. As an improved approach, our team has explored the possibility of design and preparation of a functionalized DNA origami. Built on the scaffold of the origami, we call for the conjugation with folic acid (a widely employed targeting agent for cancer cells), chlorambucil (an anticancer drug for chemotherapic action) and graphene quantum dot (a biocompatible fluorescent imaging agent) affording a potential theranostic device with targeting, diagnostic and therapeutic function. The fabricated “origami cargo” will be characterized by southern blot , TEM and fluorescence measurement. The cytotoxicity and cell uptake efficiency in living cancer cells will be explored using MTT assay and confocal microscopy.
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